Biomedical subjects

Anna Borkowska

Publications and source records attributed to Anna Borkowska.

4 recordsLinked to original sources

PubMed2006

[Prevalence of ESBL strains in urinary tract infections in children in 1996 and 2004].

Ethiological agents in urinary tract infections and prevalence of bacterial strains producing extended spectrum of beta-lactamases isolated from urine of children with urinary tract infections were compared. Patients were hospitalized in Department of Pediatry, Pediatric Gastroenterology and Oncology of Medical University of Gdańsk in 1996 and 2004. Seventy bacterial strains isolated from urine in 1996 and 113 strains isolated in 2004 were analyzed. In both years Enterobacteriaceae were the most frequently encountered with Escherichia coli predominance. Proteus spp. (10.9%), Enterobacter spp. (7.1%), Klebsiella spp. (4.4%), and Enterococcus spp. (9.3%) were isolated rarely. Among bacteria isolated in 1996 there were no ESBL strains while in 2004 ESBL strains accounted for 11.5% of all urinary bacteria. Klebsiella oxytoca (25%) and Klebsiella pneumaniae (20%) produced ESBL most frequently. ESBL production was less frequent in Escherichia coli (13.2%) and Proteus mirablilis (10%). In conclusion, among bacteria isolated from urine of children with urinary tract infections G(-) strains producing ESBL were encountered only in recent years.

Child↗

PubMedDate not supplied

Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes.

A vector system is described that combines reliable, very low level, regulated protein expression in human cells with two affinity purification tags (Sequential Peptide Affinity, or SPA, system). By avoiding overproduction of the target protein, this system allows for the efficient purification of natural protein complexes and their identification by mass spectrometry. We also present an adaptation of the SPA system for the efficient purification and identification of protein complexes in E. coli and, potentially, other bacteria.

Cells, Cultured↗

PubMedDate not supplied

[Rickets prophylaxis under current recommendations].

AIM OF THE STUDY: We analysed the compliance of current recommendations concerning rickets prevention in Poland. MATERIAL AND METHODS: The study group consisted of 65 infants hospitalised because of emergency problems. In each case, the medical history included prophylaxis of rickets and feeding pattern. In most cases (80%) we measured calcium, phosphate, and alkaline phosphatase in the serum. RESULTS: Vitamin D3 was overdosed in 70% of infants. 14% of children were diagnosed as rachitic in outpatient department before hospitalisation, but neither the clinical symptoms nor the laboratory test confirmed the diagnosis. This confirms the observation, that rickets is diagnosed too often, usually on the basis of isolated craniotabes in young infants. CONCLUSION: In the context of changing recommendations for prophylaxis of rickets, we recommend more careful prescribing of vitamin D3 preparations.

Drug Overdose↗

PubMedDate not supplied

[Antibiotics in lower respiratory tract infection].

THE AIM OF THE STUDY: Comparison of clinical outcome of lower respiratory tract infections in small children treated or not treated with antibiotics. MATERIAL AND METHODS: Eighty seven children aged from 2 months to 5 years admitted to hospital with mild to moderate bronchitis and/or pneumonia were randomly allocated to one of two groups: group A children were treated with antibiotics and group B patients were not. The children remained under close clinical observation. The clinical outcome was considered good if symptoms of the disease decreased after 72 hours. In case of no clinical improvement or worsening, the children from group B received antibiotics and in children from group A antibiotic therapy was changed. RESULTS AND CONCLUSIONS: There were no significant differences between the two groups in the course of the disease, the frequency of pulmonary complications and recurrence during one month of follow-up.

Administration, Oral↗