Biomedical subjects

Anna Kasicka-Jonderko

Publications and source records attributed to Anna Kasicka-Jonderko.

7 recordsLinked to original sources

PubMed2006

Short- and medium-term reproducibility of gastric emptying of a solid meal determined by a low dose of 13C-octanoic acid and nondispersive isotope-selective infrared spectrometry.

AIM: To evaluate the reproducibility of a modified 13C breath test-based measurement of solid phase gastric emptying (GE) within the frames of a simple-repeated measure study protocol. METHODS: Twelve healthy subjects (6 females and 6 males, mean age 24.9+/-0.7 years) were recruited to undergo three identical GE examinations. In six subjects the first two examinations were performed 2 d apart, and the third session was carried out at a median interval of 19.5 d (range 18-20 d) from the second one. In another six subjects the first two measurements were taken 20 d apart (median, range: 17-23 d), whereas the third session took place 2 d after the second one. Probes of expiratory air collected before and during six hours after intake of a solid meal (378 kcal) labelled with 75 microL (68 mg) 13C-octanoic acid, were measured for 13CO2 enrichment with the nondispersive isotope-selective infrared spectrometry NDIRS apparatus. RESULTS: Taking coefficients of variation for paired examinations into account, the short-term reproducibility of the GE measurement was slightly but not significantly better than the medium-term one: 7.7% and 11.2% for the lag phase (T-Lag), 7.3% and 10.9% for the gastric half emptying time (T1/2). The least differences in GE parameters detectable at P=0.05 level in the 12 paired examinations were 9.6 and 15.6 min for T-Lag, 11.6 and 19.7 min for T1/2 by a two-day or two to three-week time gap, respectively. CONCLUSION: The low-cost modification of the breath test involving a lower dose of 13C-octanoic acid and NDIRS, renders good short- and medium-term reproducibility, as well as sensitivity of the measurement of gastric emptying of solids.

Adult↗

PubMed2006

Comparison of multichannel electrogastrograms obtained with the use of three different electrode types.

The goal of the study was to establish if the conductive area size of recording electrodes affects the quality of a multichannel electrogastrogram. In twelve volunteers (9F, 3M, median age 24 years, range 22-28) on three separate days fasted and postprandial four-channel electrogastrograms were registered in randomized order with Red Dot class Ag/AgCl electrodes of a type: ;2222' (conductive area/total area: 2.00/10.24 cm2, ;2271' 2.54/29.64 cm2, or ;2660' 11.64/11.64 cm2 (total surface conductive!), and subsequently analyzed with a dedicated software. In the case of type 2271 and 2660 the between-electrode electrical conductivity improved at the end of the recording relative to the basal measurement, whereas type 2222 yielded a stable between-electrode electrical conductivity throughout the examination. Despite the differences in either the conductive area size or its construction, the analysis of variance on parameters describing quantitatively the multichannel electrogastrogram did not reveal a superiority of any from among the electrodes tested. Type 2271 was, however, rated the less handy among the three electrodes. Multichannel surface electrogastrography seems to be technically feasible with any type of high quality electrodes, therefore small dimensions and easy handling may favor the choice of a particular type for this examination.

Adult↗

PubMed2005

Feasibility of a breath test with a substrate of natural 13C-abundance and isotope-selective non-dispersive infrared spectrometry: a preliminary study.

BACKGROUND AND AIMS: Isotope-selective non-dispersive infrared spectrometry (NDIRS) is a cheaper alternative to isotope ratio mass spectrometry (IRMS). We checked on the efficiency of NDIRS to perform a breath test with naturally (13)C-enriched maize starch. METHODS: Fifty-six healthy volunteers were examined. After a basal fasted sample of their expiratory air was collected, the subjects ate a test breakfast of 50 g (CF50 group, n = 9) or 100 g (CF100 group, n = 47) cornflakes with 175 g yoghurt. For 6 h thereafter, exhaled air was collected every 30 min for the NDIRS measurement of (13)CO(2) concentration. RESULTS: The time-course of the curves of (13)CO(2) concentration resembled those obtained previously with IRMS. A dose-response to the amount of ingested cornflakes was observed--the maximum postprandial net increment in (13)CO(2) was statistically significantly higher in the CF100 than CF50 group: 4.78 +/- 0.13 versus 3.12 +/- 0.17 per thousand (P < 0.001), whereas the time needed to reach the maximum did not differ after the intake of 50 g (233 +/- 13 min) or 100 g (248 +/- 9 min) cornflakes. The 5-h area-under-the-curve of cumulative (13)CO(2) net recovery (AUC(60-360)) was statistically significantly greater after ingestion of 100 g compared to 50 g cornflakes (97.6 +/- 2.2 versus 61.7 +/- 2.2 micromol, P < 0.001) and it also exhibited a stable between-subject variability. CONCLUSION: The obtained proof of technical feasibility of a breath test with the use of naturally (13)C-enriched starch and NDIRS provides background for future research on the clinical usefulness of this method for a non-invasive assessment of the pancreatic exocrine function.

Adult↗

PubMed2005

Does body posture affect the parameters of a cutaneous electrogastrogram?

In a study aimed to test the effect of body position on the parameters derived from surface electrogastrograms, 17 healthy volunteers (2M, 15F; median age 22.5 years) attended in random order two examination sessions held on separate days. A 30-min recording of the interdigestive gastric myoelectrical activity (GMA) was followed by a 90-min postprandial recording after intake of a 394 kcal mixed solid-liquid test meal. For the first examination the subject was examined in a recumbent position, whereas for the second examination a sitting position was maintained. The dominant frequency and relative time occupied by normogastria was negligibly affected by the posture of the subject during GMA recording. However, a decrease in the dominant power (DP) of the gastric slow waves was observed during both the interdigestive and the postprandial recording period in a sitting position compared to a recumbent position. Consequently, the fed to fasted state DP ratio remained unaffected by body posture during GMA recording. The results indicate that by carefully observing procedural guidelines, good quality electrogastrograms can be obtained with a sitting subject, enabling the provision of comparable parameters to those achieved from standard examination in a recumbent position.

Adult↗

PubMed2004

Effect of single-dose administration of recombinant interferon-alpha2b on gastric myoelectrical activity in patients with chronic hepatitis C.

BACKGROUND: Upper abdominal complaints during interferon therapy may result from impaired gastric motility and/or evacuatory function. We examined the effect of acute administration of interferon on gastric myoelectrical activity (GMA) with the use of surface electrogastrography. METHODS: The study population comprised 25 patients with chronic hepatitis C. All of them were naive to interferon. On 2 days, after a 25-min basal GMA registration, in group A (5 men, 7 women, aged 44.3 +/- 2.8 years) placebo or 5 million i.u. recombinant interferon alpha-2b (IFNA) was administered s.c. and the GMA was recorded in the interdigestive state for two periods (2 h and 4 h), separated by a 15-min break. In group B (7 men, 6 women, aged 44.7 +/- 3.9 years) placebo or 5 million i.u. IFNA was injected s.c. after the ingestion of a semiliquid test meal of 364 kcal. Subsequently, the postprandial GMA was recorded for two periods (2 h and 4 h), separated by a 15-min break. RESULTS: A typical flu-like syndrome was observed in 91.7% of patients in group A, and in 92.3% of patients in group B, thus providing evidence of the pharmacodynamic efficiency of the IFNA administration. In the fasted state, IFNA brought about a negligible increase in the rhythmicity and power of the gastric slow waves. IFNA did not elicit any statistically significant effect on gastric slow-wave activity postprandially. CONCLUSIONS: Acute administration of interferon does not involve any deterioration of GMA that could be linked to the previously reported upper abdominal symptoms in patients undergoing treatment with this drug.

Adult↗

PubMed2004

[Non-neoplastic sequels of cigarette smoking in the digestive system].

The paper provides an up-to-date report on the effects of cigarette smoking on the secretory, motor, and transport functions of the gastrointestinal tract in humans. Pathophysiological linkage between the deleterious impact of cigarette smoking on the gastrointestinal tract and several digestive tract diseases is pointed out. Evidence for a decisive role of nicotine in cigarette smoking evoked distortions of gastrointestinal tract functioning is provided.

Gastrointestinal Diseases↗

PubMed2003

[Application of the hydrogen breath test for the evaluation of oro-caecal transit time].

Physiological background and performance of the hydrogen breath test are described. Clinical and research applications of the hydrogen breath test for evaluation of the transport function of the small bowel are pointed out. Several factors affecting the oro-caecal transit time, such as: physiological (age, sex, menstrual cycle), usage of stimulants (coffee, alcohol, cigarette smoking), as well as pathological conditions, are discussed. An in-depth review of drug effects on the oro-caecal transit time is provided.

Breath Tests↗