Biomedical subjects

Anne Norwood

Publications and source records attributed to Anne Norwood.

3 recordsLinked to original sources

PubMed2006

Generational (age) differences in nursing students' preferences for teaching methods.

A generational age transformation is occurring in nursing classrooms across the United States. Nurse educators need to prepare for the different values and expectations of students from Generation X and the newly emerging Generation Y in the educational environment. This quantitative, descriptive research begins to examine the preferences and expectations of these generations regarding teaching methods.

Adolescent↗

PubMed2006

The role of glucosamine, chondroitin and thymoquinone on the viability and proliferation of a HTB-93 rheumatoid arthritis cell model.

Glucosamine (GS), chondroitin (CD), and thymoquinone (TQ) are the complementary medicines under investigation in this study. Glucosamine is a naturally occurring glycoaminoglycan that contributes to the development of proteoglycans needed for the development of cartilage development and regeneration. Chondroitin is also a naturally occurring glycoaminoglycans (GAG) that seems to support the efforts of glucosamine as well as provide chondroprotection while serving as a 'water magnet' within the joint matrix. Thymoquinone is derived naturally from the black seed plant that is extremely popular within Middle Eastern countries. Its benefits are multiple, including both antioxidant and anti-inflammatory properties. These products were administered to HTB-93 synovial cells and cell viability, damage, and alterations in morphology were analyzed after 72 hours. Preliminary results revealed that chondroitin increased cell number in the high treatment group with increased nitric oxide production and decreased glutathione content compared to the control, glucosamine, and TMQ. Decreased glutathione levels were seen in the medium and high doses of both glucosamine and chondroitin. Increased levels of glutathione were seen with increasing TMQ, without changes in cell numbers or nitric oxide. The data indicates that medium and high doses of glucosamine and chondroitin may be cytotoxic to HTB-93 synovial cells.

Arthritis, Rheumatoid↗

PubMed2006

Effects of (-)epigallocatechin gallate and thymoquinone on proliferation of a PANC-1 cell line in culture.

The limited ability of current treatments for pancreatic cancer prompted us to examine the effects of antioxidants on proliferation of pancreatic cancer cells. Antioxidants have been reported to possess antioxidant activity in vitro. The specific aim of this study was to investigate the role of epigallocatechin gallate (EGCG), a major component of green tea and thymoquinone on the proliferation and viability of PANC-1 cell line using a standard method of treatment. The PANC-1 cells were treated with three predetermined doses of thymoquinone and EGCG (5, 25, and 50 ug/dL) for 24, 48, and 72 hours in culture medium. Determination of viability and morphology was examined microscopically after each 24 hour interval. Data collected from this study indicated a dose dependent relationship with direct administration of EGCG alone or in combination with thymoquinone. However, a rebound effect was observed after 48 and 72 hours during direct administration of both antioxidants. These results indicate that direct administration of EGCG alone or in combination with thymoquinone can limit PANC-1 cell proliferation (p < 0.05). EGCG and thymoquinone may be a potent biologic inhibitor of human pancreatic carcinomas, reducing their propagation activities.

Adenocarcinoma↗