Biomedical subjects

Annie Schmied

Publications and source records attributed to Annie Schmied.

7 recordsLinked to original sources

PubMed2006

Cortical versus spinal dysfunction in amyotrophic lateral sclerosis.

Little is known about the possible link between cortical and spinal motor neuron dysfunction in amyotrophic lateral sclerosis (ALS). We correlated the characteristics of the responses to transcranial magnetic stimulation (TMS) with the electromechanical properties and firing pattern of single motor units (MUs) tested in nine ALS patients, three patients with Kennedy's disease, and 15 healthy subjects. In Kennedy's disease, 19 of 22 MUs were markedly enlarged with good electromechanical coupling and discharged with great variability. Their excitatory responses increased with MU size. In ALS, 17 of 34 MUs with excitatory responses behaved as in Kennedy's disease. By contrast, 28 MUs with nonsignificant responses showed poor electromechanical coupling and high firing rates, whereas 28 MUs with inhibitory responses showed moderate functional alterations. This result indicates that in ALS as in Kennedy's disease, sprouting of corticospinal axons may occur on surviving motoneurons. A clear relationship exists between the responsiveness of MUs to TMS and their functional state.

Adolescent↗

PubMed2004

Changes in the inhibitory control exerted by the antagonist Ia afferents on human wrist extensor motor units during an attention-demanding motor task.

The aim of this study was to determine the extent to which an attention-demanding visuomotor task affects the strength of the inhibitory control exerted by the wrist flexor group Ia afferents on the wrist extensor motoneurons. Effects of median nerve stimulation on the tonic activity of wrist extensor single motor units were analyzed in terms of the interspike interval (ISI) lengthening. Results show that the inhibitory effects exerted by the antagonistic group Ia afferents were significantly enhanced when the wrist extensor motoneurons were involved in an attention-demanding task. Enhanced inhibition from antagonist afferents may reflect task-related changes in the excitability of the di- and/or polysynaptic pathways mediating reciprocal inhibition due to either the action of descending inputs and/or an increase in the efficiency of the Ia inputs to the premotoneuronal inhibitory interneurons. Modulation of the inhibition exerted by proprioceptive antagonist afferents may be one of the processes which contribute to the fine adjustment of the wrist muscle force output required in fine handling tasks.

Action Potentials↗

PubMed2004

Proprioceptive control of human wrist extensor motor units during an attention-demanding task.

The responsiveness of the tonically firing single motor units (SMU) to Ia afferent volleys elicited by either mechanical (T-reflex) or electrical nerve stimulation (H-reflex) was tested in the extensor carpi radialis muscle (ECR) while the subjects were maintaining a steady wrist extension force using visual feedback set either at low or high gain. The aim was to determine whether the proprioceptive control of tonic motoneuronal activity depends on the level of attentiveness required by the behavioural context. The response probability of the SMUs to tendon taps was significantly higher (p<0.0001) and that to electrical nerve stimulation was lower (p<0.001) during the more demanding task. Since these changes in SMU responsiveness were not accompanied by any differences in either the motor unit firing patterns or the mean levels of EMG muscle activity, it can be concluded that there were no attention-related changes in the net excitatory drive to the ECR motoneurons. These results are consistent with the idea that fusimotor sensitization of the muscle spindle may have occurred in the more demanding task: an increase in the mechanical sensitivity of the muscle spindles would certainly account for both the T-reflex facilitation and the H-reflex depression observed. The attention-demanding task therefore seemed to involve an independent fusimotor drive activation process. The results of this study suggest that an adaptation of the fusimotor system occurs in humans, depending on the levels of attention and accuracy required to perform the ongoing motor task, as previously reported to occur in animals.

Action Potentials↗

PubMed2003

Recurrent inhibition of wrist extensor motoneurones: a single unit study on a deafferented patient.

In order to document the effects of recurrent inhibition on the firing times of human alpha-motoneurones during natural motor behaviour, a case study was performed on a deafferented patient. The fact that this subject had completely lost the large-diameter sensory afferents provided us with a unique opportunity of selectively stimulating the motor axons in the nerves. The tonic activity of single motor units (n = 21) was recorded in the extensor carpi radialis muscles while applying randomly timed antidromic electrical stimuli to the radial nerve. The peristimulus time histogram analysis showed the presence of biphasic inhibitory effects, including an early, short-lasting component followed by a longer-lasting component occurring 20-40 ms later. The interspike interval (ISI) during which the stimulation occurred was generally lengthened as compared to the previous ISIs. The stimulation was most effective when delivered early (20-30 ms) after a spike. It was also effective, although less so, when delivered at the end of the ISI (70-100 ms after a spike). The lengthening effect sometimes extended over one or two of the subsequent ISIs. The lengthening effect of the motor axon stimulation was followed by an excitatory-like effect, which took the form of a shortening that affected up to five ISIs after the stimulation. The biphasic inhibitory effects and the subsequent facilitatory effects are discussed in terms of the dual nature of the synaptic processes involved in the recurrent inhibitory network, the postactivation facilitation/depression processes and the mutual inhibition occurring between Renshaw cells.

Action Potentials↗

PubMed2003

Pharmacologically induced enhancement of recurrent inhibition in humans: effects on motoneurone discharge patterns.

The aim of the present study was to investigate the effects of spinal recurrent inhibition on human motoneurone discharge patterns. The tonic discharge activity of motor unit pairs was recorded in the extensor carpi radialis (ECR) and abductor digiti minimi (ADM) muscles during voluntary isometric contraction. While undergoing continuous intravenous saline (NaCl 0.9 %) perfusion, the subjects were given a short lasting injection of L-acetylcarnitine (L-Ac), which has been found to potentiate recurrent inhibition in humans. The variability, synchronization and coherence of the motor unit discharges were analysed during four successive test periods (lasting 2-3 min each). A significant decrease in the inter-spike interval (ISI) coefficient of variation was observed in the discharge patterns of the motor units tested in the ECR and not in the ADM, which were not accompanied by any consistent changes in the mean ISIs of the motor unit activity in either muscle. The L-Ac injection also led to a significant increase in the synchronization in half of the motor unit pairs tested in the ECR muscle (n = 29), whereas no consistent changes were observed with the ADM motor units (n = 25). However, coherence analysis failed to reveal any consistent differences in the incidence of significant values of coherence spectrum between the pre-injection and injection periods among the motor unit pairs tested with either saline or L-Ac injections, in either the ECR or ADM muscles. The contrasting effects on the variability and the synchronization of the motor unit discharges observed with ECR motoneurones known to undergo recurrent inhibition and with ADM motoneurones known to lack recurrent inhibition suggest that the drug may have specific effects which are mediated by an enhancement of the Renshaw cell activity. The decrease in the ISI variability is in line with the hypothesis that recurrent inhibition may contribute along with the post-spike after-hyperpolarization to limiting the influence of the synaptic noise on the firing times of steadily discharging motoneurones. The present data, which suggest that recurrent inhibition plays a synchronizing rather than a desynchronizing role, are in keeping with the fact that the Renshaw cells may provide an important source of common inhibitory inputs.

Acetylcarnitine↗

PubMed2002

Delayed and prolonged effects of a near threshold EPSP on the firing time of human alpha-motoneurones.

In order to investigate the effects of near-threshold excitatory inputs on the precise timing of the action potentials during the tonic discharge of human motoneurones, the activity of single motor units was recorded in the extensor carpi radialis muscles while tendon taps (indentation, 0.1 mm; duration, 1 ms) were being delivered irregularly at a mean rate of 0.8 s(-1). New methods of analysis, such as the phase response function, were used to study the relative changes in the interspike interval (ISI1) during which the stimulus was being delivered and in the three subsequent intervals (ISI2, ISI3, ISI4) as a percentage of the pre-stimulus interspike interval (ISI0). The consistency of the effects of the actual stimulus as regards the spontaneous variability was assessed by comparing the data with those obtained with virtual stimulation. When the stimulus occurred at the end of ISI1, and triggered a spike, ISI1 and ISI3 were generally shortened, whereas ISI2 was lengthened, probably due to the negative correlation induced by the summation of the after-hyperpolarisations (AHPs). When the stimulus occurred in the middle of ISI1 without triggering a spike, ISI1, ISI2 and more rarely ISI3 were shortened. Lastly, when the stimulus occurred during the AHP scoop in ISI1, ISI2 was shortened although ISI1 remained unchanged. ISI4 was not consistently affected in any of these cases. The present results show that the tendon tap-induced inputs (probably from muscle spindle primary endings) mediated delayed and prolonged shortening effects of the ISIs on most of the alpha-motoneurones tested (n = 16). These effects undetected in classic peri-stimulus histogram analysis may involve long-lasting conductance changes although the contribution of polysynaptic pathways cannot be excluded. The changes in ISI were quite moderate (< 15% of ISI) but highly consistent. Their functional involvement in the synchronisation or desynchronisation processes and/or the mechanisms of optimisation of muscle contraction still remains to be explored.

Action Potentials↗

PubMed2002

Presynaptic and disynaptic inhibition induced by group I muscle afferents.

The task related changes in the Gp I inputs were investigated in type-identified motor units in the wrist extensor muscles. During wrist extension, the monosynaptic inputs generated by applying radial nerve stimulation were distributed among the motoneurone pool in line with the size principle. Their effectiveness was enhanced in the same way during hand clenching and during wrist extension combined with stimulation of the palm and finger cutaneous receptors. The orderly distribution of the monosynaptic Gp I inputs was reversed by the presynaptic inhibition induced by stimulating the Gp I flexor afferents. The effects of the presynaptic inhibition were partially released by applying cutaneous stimulation. During wrist extension, the Gp I flexor afferents generated disynaptic excitatory inputs acting specifically on high-threshold motor units together with disynaptic inhibitory inputs distributed in line with the size principle among the wrist extensor motor nucleus. During hand lenching, their effectiveness was differentially modulated depending on the motor unit type.

Animals↗