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Antigone S Skopelitou

Publications and source records attributed to Antigone S Skopelitou.

6 recordsLinked to original sources
PubMed2003

Immunohistochemical expression of FHIT gene product in inflammatory bowel disease: significance and correlation with clinicopathological data.

OBJECTIVE: To investigate the expression of Fhit protein in 53 patients with ulcerative colitis and Crohn's disease, as well as in 13 ulcerative-colitis-associated adenocarcinomas, and its eventual relationship with clinicopathological data and response to therapy. MATERIALS AND METHODS: We performed immunohistochemistry in archival material of formalin-fixed, paraffin-embedded tissues using the anti-Fhit antibody and the streptavidin-biotin peroxidase method. RESULTS: In 35/38 cases of ulcerative colitis, Fhit protein (pFhit) was absent or reduced. In the remaining three cases, it was expressed normally. In 12/15 cases of Crohn's disease, pFhit was absent or reduced, as it was in 4/13 cases of ulcerative-colitis-associated adenocarcinoma. Statistically significant differences of pFhit expression were observed between the active phase and the chronic healed phase of ulcerative colitis, and between the active phase and the normal colon mucosa. Also, statistically significant differences of pFhit expression were observed between (1) the resolving phase of ulcerative colitis and normal colon mucosa, (2) the chronic healed phase and normal colon mucosa, and (3) Crohn's disease and normal colon mucosa. Interestingly, a statistically very significant difference in pFhit expression was noticed between ulcerative colitis and ulcerative-colitis-associated adenocarcinoma. CONCLUSIONS: Our results show that immunohistochemical expression of pFhit is completely absent or very reduced in idiopathic bowel disease (IBD) as well as in several ulcerative-colitis-associated adenocarcinomas in north-western Greece. These findings suggest that the FHIT gene might be involved in IBD and in a subgroup of ulcerative-colitis-associated carcinogenesis, although larger series should be tested.

Acid Anhydride Hydrolases↗
PubMed2003

Immunohistochemical expression of Fhit protein in Helicobacter pylori related chronic gastritis, gastric precancerous lesions and gastric carcinoma: correlation with conventional clinicopathologic parameters.

OBJECTIVE: Our purpose was to investigate the immunohistochemical expression of Fhit protein in chronic Helicobacter pylori related gastritides, gastric epithelial dysplasia and gastric carcinoma. DESIGN: Because a subset of chronic H. pylori related gastritides, precancerous lesions and gastric tumours were found to be either entirely negative or to minimally express Fhit protein, it is suggested that alteration of the carcinogen susceptible fragile region within the FHIT gene is an early event in a subset of gastric adenocarcinomas. METHODS: We carried out immunohistochemical tests on archived material of formalin fixed, paraffin embedded tissues, using the anti-FHIT antibody and the streptavidin-biotin-peroxidase method, in a total of 135 gastric lesions (76 biopsies and 59 surgically resected gastric adenocarcinomas). RESULTS: In this study, 79% of H. pylori related gastritides showed that the Fhit protein was either completely absent or there was a marked reduction of immunostaining. Similar results were obtained for 76.4% of cases of chronic gastritides with low and high-grade dysplasia, and 56% of gastric adenocarcinomas. A negative result for Fhit protein immunostaining was strongly associated with H. pylori infection (P = 0.0001) and with epithelial dysplasia (P = 0.01) but not with intestinal metaplasia or degree of activity. Additionally, negative or reduced immunostaining for Fhit was associated with the degree of dysplasia and progress of a subset of these lesions to infiltrating adenocarcinoma (P = 0.02). In gastric adenocarcinomas negative or weak immunostaining of the Fhit protein correlated with the histological grade (P = 0.01) and clinical stage of the disease (P = 0.01). CONCLUSIONS: Our results suggest that, because Fhit protein immunostaining is completely absent in (1) the majority of H. pylori related chronic gastritides, (2) a subset of gastric dysplastic lesions, and (3) a proportion of primary gastric adenocarcinomas, irrespective of histological type, it can play an important role in the early development and progression of some gastric cancers.

Acid Anhydride Hydrolases↗
PubMed2002

Small bowel adenocarcinoma presenting as a first manifestation of Crohn's disease: report of a case, and a literature review.

Small bowel Crohn's disease has been related to an increased incidence of small bowel adenocarcinoma, but the total number of reported cases is small. We present an interesting case of a young male patient with nephrolithiasis in childhood, an atypical intermittent history of diarrhoea also since his childhood, who developed obstructive ileus and underwent an urgent operation. The operation revealed a stenosis of the ileum owing to a mass, which proved to be a small bowel adenocarcinoma. One month later, the patient underwent a curative surgical resection of the tumour with additional lymphadenectomy, followed by chemotherapy for 6 months. Since then, the patient had mild diarrhoea but enteroclysis was normal. Approximately 2 years after the resection of the tumour, a new ileocolonoscopy demonstrated deep ulcerations of the terminal ileum and the adjacent area of the colon as well as anal ulcerations. The new biopsy specimens were convincing for Crohn's disease. There were no signs of residual or relapsing cancer. There is growing evidence that Crohn's disease is one of the triggering factors for the development of small bowel adenocarcinoma. Underlying Crohn's disease should be suspected in a young patient with an atypical history of diarrhoea and small bowel adenocarcinoma.

Adenocarcinoma↗