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Antoni Basta

Publications and source records attributed to Antoni Basta.

17 recordsLinked to original sources
PubMed2006

The obstetrical history in patients with Pfannenstiel scar endometriomas--an analysis of 81 patients.

INTRODUCTION: The participation of immune tolerance during pregnancy was suggested to be an important factor predisposing to the implantation of decidual cells after cesarean section in Pfannenstiel scar. Delivery at term is related to the termination of immune tolerance to fetal antigens that is maintained throughout pregnancy. Substantial proportion of cesarean section deliveries is performed before the onset of true term labor. The aim of this study was to analyze the clinical symptoms of spontaneous beginning of labor in pregnant women in whom cesarean sections were performed and in whom Pfannenstiel scar endometriomas were observed during follow-up. MATERIALS AND METHODS: We have retrospectively analyzed 81 patients following the surgical removal of scar endometrioma after cesarean section. Obstetrical histories of cesarean sections in the number of 5,370 preceding the occurrence of the scar endometrioma were analyzed. These data were collected in six different Gynecological and Obstetrical wards in Malopolska Province in Poland. Analysis of data was started by the retrospective evaluation of regular uterine contractions, uterine cervix ripening before cesarean section and the indications for surgery. RESULTS: In 67 women from the group of 81 patients cesarean sections were performed with unripe uterine cervix and without the presence of regular uterine contractions. Elective indications for cesarean sections were predominant in this group of women. The relative risk of scar endometriomas occurrence following cesarean sections performed before onset of labor in comparison to cesarean sections following spontaneous onset of labor was statistically significantly higher [RR = 2.16, 95% CI = 1.21-3.83; OR = 2.18, 95% CI = 1.22-3.89]. CONCLUSIONS: Cesarean section performed before spontaneous onset of labor may increase substantially the risk of occurrence of scar endometriomas.

Adult↗
PubMed2006

Effects of oral L-arginine on the foetal condition and neonatal outcome in preeclampsia: a preliminary report.

Estimation of the influence of oral supplementation with low dose of L-arginine on biophysical profile, foeto-placental circulation and neonatal outcome in preeclampsia. Randomized, placebo-controlled, double-blind, clinical trial. Oral therapy with 3 g of L-arginine daily or placebo as a supplement to standard therapy. Eighty-three preeclamptic women, randomly assigned to the L-arginine (n=42) or placebo (n=41) groups; [n=30 (L-arginine) and n=31 (placebo) ended the study, respectively]. Foetal gain chances due to ultrasound biometry, biophysical profile, Doppler velocimetry of pulsatility indices of umbilical and middle cerebral arteries, cerebro-placental ratio, as well as differences in duration of pregnancy and clinical data of newborn. L-arginine treatment transitory accelerated foetal gain and improved biophysical profile. Starting from 3rd week of therapy, the umbilical artery pulsatility indices values were significantly lower in L-arginine than in placebo group. Moreover, treatment with L-arginine caused significant increase of middle cerebral artery pulsatility indices and cerebro-placental ratio values. Latency was longer in L-arginine group. Neonates delivered in the L-arginine group revealed higher Apgar score. Supplementary treatment with oral L-arginine seems to be promising in improving foetal well-being and neonatal outcome as well as in prolonging pregnancy complicated with preeclampsia. However, these benefits require confirmation in more-powered, larger studies.

Administration, Oral↗
PubMed2006

[Comparative analysis of CD56 lymphocytes and CD69 antigen expression in endometrium during decidualization].

INTRODUCTION: The exceptionality of endometrium, differentiating it from other epithelial tissues is related with its ability to regulate the number and activity of infiltrating immunological cells according to menstrual cycle phases. The aim of this study was a comparative evaluation of the number of CD56 positive cells, as well the analysis of CD69 antigen expression during decidualization. MATERIALS AND METHODS: The expression of CD56 and CD69 antigens was analyzed using the immunohistochemistry method in 20 decidual tissue samples derived after curettage following spontaneous abortion and in 38 endometrial tissue samples obtained during hysterectomy performed because of benign myoma diagnosis. The endometrial tissue samples were divided regarding to the menstrual cycle phases. Following phases were distinguished: early proliferative, mid-proliferative, late proliferative, early secretory, mid secretory and late secretory. RESULTS: The higher CD56 positive cells were recognized in the endometrium during mid secretory cycle phase. The number of CD56 positive cells was significantly lower in endometrium, independent of menstrual cycle phase in comparison to endometrium following abortion. The expression of CD69 antigen was observed to increase beginning with early secretory cycle phase and remained at high level during the secretory cycle phase and the abortion. CONCLUSIONS: The observed differences in the number of CD56 positive cells during decidualization without the changes in immune cells activity evaluated using CD69 antigen expression may indicate the participation of activated mononuclear cells in the normal development of pregnancy beginning with implantation.

Abortion, Spontaneous↗
PubMed2006

[Pregnancy complicated with thrombocytopenia].

OBJECTIVE: Evaluation of the influence of the sort of thrombocytopenia and count of platelets on the course of pregnancy, maternal and neonatal outcomes. DESIGN: Retrospective analysis of 45 women, threatened of thrombocytopenia. MATERIALS AND METHODS: The trial population consisted of 45 women, where the following parameters were evaluated: age of laboring women, gestational age, the mode of delivery, count of platelets, bleeding complications, the need of treatment with steroids and platelets transfusions, as well as the birth weight of newborns, the Apgar score in the 1st minute, thrombocytopenia and bleeding complications in neonates. RESULTS: There were 27 women with gestational-induced thrombocytopenia and 18 women had immune thrombocytopenia. Moreover 14 had severe and 31--moderate thrombocytopenia. Premature labor (<37 week of pregnancy) were observed more often in patients with severe than in moderate thrombocytopenia (6/14-42,85% vs. 4/31-16,13%; p < 0.05). Lower neonatal body weight (2774.28 +/- 28 vs. 3120.32 +/- 788.22; p < 0.05), Apgar score in the 1st minute (7.42 +/- 3.56 vs. 9.13 +/- 1.56; p < 0.01), as well as neonatal complications (71.42% vs. 28.58; p < 0.01), were also observed more often in severe than in moderate thrombocytopenia group. CONCLUSIONS: The presence of antiplatelet antiobodies were associated with platelets count <50 x 10(3)/MICROL, the necessity of treatment with steroids and platelet infusions to the mother, as well as delivery of neonates with lower Apgar score in the 1st minute and neonatal thrombocytopenia. Severe thrombocytopenia was associated with the necessity of treatment with steroids and platelet infusions, preterm delivery, lower Apgar score in the 1st minute and neonatal thrombocytopenia.

Adult↗
PubMed2005

Metallothionein and RCAS1 expression in comparison to immunological cells activity in endometriosis, endometrial adenocarcinoma and endometrium according to menstrual cycle changes.

OBJECTIVE: Endometrium is a specialized organ in which phenomena controlling the level of cell proliferation and apoptosis are marked. The aim of our study was to determine the presence of proteins involved in apoptosis and proliferation: RCAS1, MT and the number of CD56-positive cells and their activity to elucidate their possible role in the development of adenocarcinoma and endometriosis. MATERIALS AND METHODS: MT, RCAS1, CD56-positivity and CD69 expression were assessed in 55 tissue samples by Western blot and immunohistochemistry methods. RESULTS: We found that endometrium during secretory menstrual cycle phase is characterized by significantly higher RCAS1 and higher MT expression than in proliferative phase. The number of CD56-positive cells and the CD69 antigen expression was significantly increased. Endometrial adenocarcinoma was characterized by significantly increased RCAS1 expression, while MT expression was comparable to the level found in the secretory phase. The number of CD56-positive cells was significantly decreased and their activity was comparable to the level found in the secretory phase. Endometriosis was accompanied by significantly lower RCAS1 and MT expressions, with lower number of CD-56 positive cells and lower expression of CD69 antigen in comparison to the secretory phase. CONCLUSIONS: The ability of endometrium to determine cytotoxic activity (RCAS1 expression changes) and high protection against DNA damage (MT expression) with concomitant changes in the number of immune cells and their activity, observed in normal endometrium during the menstrual cycle phases seems to be fundamental for pathological features of endometrial adenocarcinoma and endometriosis.

Adenocarcinoma↗
PubMed2003

[Pregnancy and delivery after hysteroscopic metroplasty].

AIM OF THE STUDY: Outcomes of treatment of habitual miscarriages and course of pregnancy and delivery in women after hysteroscopic metroplasty i.e. hysteroscopic resection of uterine septum. MATERIAL AND METHODS: Material consisted of 31 women with uterine septum operated between years of 1995-2002 because of habitual miscarriage by surgical hysteroscopic technique. The control group consisted of 26 women treated between years of 1990-1997 by Strassman's operation. Outcomes of treatment of habitual abortions, course of pregnancy and delivery were analyzed in both groups. The statistical analysis was performed by Chi 2 test (p < 0.05). RESULTS: After such treatment 27 out of 31 hysteroscopic treated women became pregnant. In first pregnancy in 12 women miscarriages and in 3 cases premature delivery were observed. Twelve pregnant women delivered pregnancy till 37 week. 15 infants survived (3 premature baby). Out of 26 women in control group after Strassman's operation 24 became pregnant, but 12 had miscarried. All pregnancy in control group i.e. ten mature pregnancy and 2 during premature delivery were ended by Cesarean section. The efficacy of treatment of habitual miscarriage due to uterine septum by hysteroscopic surgery was 55.5%, and there was no statistical significant difference between hysteroscopic surgery and Strassman's operation (50.0%). CONCLUSIONS: On the basis of result obtained the hysteroscopic metroplasty should be method of choice in the treatment of the uterine septum. Although the vaginal delivery is preferred one should have in mind the late complications of hysteroscopic surgery.

Abortion, Habitual↗
PubMed2003

[Coexistence of VIN and vulvar invasive cancer with intraepithelial neoplasia and invasive carcinoma of the cervix and/or vagina, and HPV infection of the low female genital tract].

INTRODUCTION: A frequency of VIN and vulvar carcinoma is continuously increasing, especially in young women in whom the changes are often multifocal. AIMS: Evaluation of VIN and vulvar carcinoma coexistence with intraepithelial as well as invasive changes within vagina and uterine cervix and estimation of the frequency of HPV infection accompanying these changes. MATERIAL AND METHODS: Study group was consisted of 389 women aged 22-92 y. Cytologic, colposcopic examinations of the entire low genital tract and diagnosis of HPV infection with use of DNA HPV hybridization technique were performed in all cases. There were 148 (38.0%) cases of vulvar changes in women below 45 yrs of life, and 241 (62.0%) cases in older patients. HPV infection was diagnosed in 91 (61.5%) young women and in 41 (17.0%) women over 45 yrs. Intraepithelial neoplasia or invasive cancer of vagina and/or uterine cervix were diagnosed in 16 (10.8%) cases of young patients, and HPV infection of high oncological potential was diagnosed in 13 (81.3%) of these patients. CONCLUSIONS: There is a high percent of coexistence of intraepithelial as well as invasive changes of the vulva, vagina and uterine cervix which mostly coexist with HPV infection of high oncological potential. These data suggest an important role of HPV infection in low female genital tract carcinogenesis and obliges to a precise examination of the entire low female genital tract, especially in young women.

Adult↗
PubMed2003

[Sentinel node biopsy in breast cancer and primary tumor dimension].

INTRODUCTION: Sentinel lymph node (SLN) biopsy, primary used as an alternative to elective lymph node dissection in melanoma, is being applied successfully in management of patients with breast cancer. The aim of this method in breast cancer is to determine the presence of axillary node involvement while clinically normal axilla. The validity of the sentinel node concept in breast cancer is demonstrated in various studies. The results of identification rates, sensitivity, overall accuracy and false negative rate are so encouraging in most publications, as to implement this concept to every-day surgical practice. There is lack, however, of many fundamental answers pertaining relationship between tumor-size and the results of SLN Biopsy or related surgical standards. MATERIALS AND METHODS: 36 females at the age 33-66, with breast cancer underwent primary surgical treatment with SLN Biopsy in Department of Gynaecology and Oncology Jagiellonian University between 2001-2002. The study inclusion criteria were tumour size-T1, T2. The day before surgery the static scanning was performed after injection of Tc radiolabelled nannocolloid. SLN(s) were identified intraoperatively using a handheld gamma detection probe (Navigator GPS) and intraoperative lymphatic mapping with blue dye (Patent Blau V). After localization and excision of SLN(s), axillary's lymph node dissection (ALND) was performed. RESULTS: In 34 patients SLN Biopsy revealed accumulation of the tracer in axilla, which was classified as SLN. Detection rate was 94.4% (34/36). Overall sensitivity of the procedure was 81%, whereas negative predictive value 92%. False negative rate was 2.2%. Detection rate for T1 tumors was 100% (15/15 cases), and for T2 tumors was 90.4% (19/21 cases). Sensitivity for tumor classified as T1 was 100% (3/3 cases), whereas for tumor T2 was 75% (6/8 cases). CONCLUSION: SLN Biopsy seems to be very interesting alternative to ALND in patients with small tumor's dimension.

Adult↗
PubMed2003

[Role of immunomodulatory treatment with Iscador QuS and Intron A of women with CIN1 with concurrent HPV infection].

OBJECTIVE: The paper presents the role of immunomodulatory treatment with Iscador QuS and Intron A of women with CIN1 and CIN2 with concurrent HPV infection. MATERIAL AND METHODS: Clinical material consisted of 96 women aged 18-52 years of life. The women were divided into three groups. Group A (35 women) treated with Iscador QuS administered s.c. twice a week for 3 months, group B (30 women) treated with Intron A, administered twice a week in the cervical injections for 3 months and control group K (31 women) without treatment followed up with cytology and colposcopy. RESULTS: In the group A (Iscador QuS) CIN remission was observed in slightly higher percentage (non significant) comparing to the control group. In the group B (Intron A) remission CIN was observed in 24 (80%) cases which was statistically significant comparing to the control and A groups. There were no progression of CIN in the group B and the stationery process was observed statistically more frequent comparing to the control and A groups. There was observed statistically higher percentage of cases without HPV infection in all groups during the experiment. The remission concerned both high and low oncogenic potency viruses. In the highest percentage CIN with concurrent HPV infection remission was observed in the B (Intron A) group. CONCLUSIONS: 1/Iscador QuS and specially Intron A increases the CIN1 and CIN2 remission rate. 2/These two agents may also affect the HPV remission.

Adjuvants, Immunologic↗
PubMed2003

The impact of progesterone on simultaneous, local secretion of IGFBP-3 and IGF-I [IGFBP-3/IGF-I index] by human malignant and non-malignant breast explants depends on tissue steroid receptor phenotype.

OBJECTIVES: Insulin-like growth factor-I (IGF-I) is regarded as one of mammary tissue proliferative factors. Insulin-like growth factor binding protein-3 (IGFBP-3) limits the IGF-I binding potential to its receptor. That limits the IGF-I bioavailability. Recently experimental studies indicated that insulin-like growth factor binding proteins (IGFBPs) might have their own biological actions beyond their ability to regulate insulin-like growth factors (IGFs). Our earlier results showed the progesterone-induced rise in hGH and IGF secretion by human breast cancer explants. AIM: To determine the ability of progesterone to stimulate simultaneous local IGF-I and IGFBP-3 secretion by non-malignant and malignant mammary tissue collected from different receptor phenotype tumours. MATERIAL AND METHODS: Explants from the tumour and surrounding normal non-malignant tissue were obtained during surging. Breast cancer explants were defined as: ER+ PR+; ER-PR-; ER+ PR-; and ER-PR+. Part of the explants was fixed in 10% buffered formalin for steroid receptor determination by immunohistochemistry. Other parts were cut into small pieces, weight and cultured in Parker medium (M199) supplemented with 5% of calf serum at 37 degrees C in an atmosphere containing 5% CO2 for 48 hours in control medium or with the addition of progesterone (10-7 M). Later media were collected for IGF-I and IGFBP-3 concentration analysis. RESULTS: Progesterone increased (p < 0.01) IGFBP-3/IGF-I index in ER(-)PR(-) non-malignant tissue and decreased the IGFBP-3/IGF-I index in ER(-)PR(+), ER(+)PR(-) non-malignant explants. That increased the IGF-I bioavailability. Breast malignant explants showed the progesterone induced IGFBP-3/IGF-I index decrease. The decrease was most evident (p < 0.01) in malignant explants expressing progesterone receptor. CONCLUSION: Progesterone increased local IGF-I bioavailability in malignant breast tissue. That phenomenon depended on steroid receptor phenotype of breast tissue and was most evident in tissue expressing progesterone receptor. In non-malignant tissue that phenomenon was also found in estrogen receptor expressing tissue. Lack of steroid receptor expression in breast explants reversed that phenomenon.

Biomarkers, Tumor↗
PubMed2003

[Lymph node mapping and sentinel node detection in carcinoma of the cervix, endometrium and vulva].

OBJECTIVES: Validity of the sentinel node concept in patients with cervical, endometrial and vulvar cancer. MATERIAL AND METHODS: 47 cases of FIGO stage I and II cervical cancer, 33 cases of first clinical stage of endometrial cancer and 37 patients with FIGO stage I and II of vulvar cancer. In cervical and vulvar cancer preoperative lymphoscyntygraphy and intraoperative lymphatic mapping with blue dye and handheld gamma probe were performed. In patients with endometrial cancer intraoperative lymphatic mapping with blue dye injected into the cervix and into the uterine corpus subserously were done. In the last 10 cases radiolabeled nannocolloid were administered and the patients underwent preoperative lymphoscyntygraphy and intraoperative radio detection of sentinel node. Sentinel nodes were labeled as blue, radioactive, or blue/radioactive. RESULTS: In cervical cancer sensitivity of the dye and radiocolloid methods was 94%, specificity 100% and negative predictive value 97%. Out of 33 cases of endometrial cancer sentinel node was identified in 29 (87.87%) patients. None of women with histological negative sentinel node had metastases in the rest of lymph nodes resected. Sentinel node was detected in all cases of vulvar cancer. The status of sentinel nodes were representative for all lymph node resected. CONCLUSIONS: Concept of sentinel node may be applied first of all for vulvar cancer and also for cervical and endometrial cancer.

Endometrial Neoplasms↗
PubMed2003

Use of Tc99m-nanocolloid for sentinel nodes identification in cervical cancer.

BACKGROUND: The initial draining lymph node for a primary tumor is referred to as the "sentinel" node. Firstly adopted in the management of patients with cutaneous melanoma and breast cancer, it is now widely tested in cervical cancer. In patients with cervical cancer, lymph node status is the most important prognostic factor for survival. In patients with cervical cancer FIGO stage I and II pelvic lymph node metastases are expected in 0-16 and 24.5-31% and para-aortic lymph node metastases are expected in 0-22 and 11-19% of patients. The removal of pelvic and para-aortic lymph nodes is essential for assessing the biology of the disease. Lymphoscintigraphy enables the visualisation of lymphatic drainage patterns from a great variety of tumour sites prior to surgery. Therefore, the current procedure is to perform the pre-operative mapping of sentinel nodes by static and/or dynamic lymphoscintigraphy, followed by in vivo identification using a gamma detection probe and selective surgical resection. MATERIAL AND METHODS: Between 2001-2003, 37 patients with cervical cancer FIGO stage I-IIa were seemed to be qualified to undergo lymphoscintigraphy. The day before surgery (99m)Tc-nanocolloid (100 MBq; 0.5-1.0 ml in volume) was applied in each quadrant of the cervix or around the tumor. The static scintigraphic scans were performed after 2 hours p.i. using a dual-head large-field-of-view Siemens gamma-camera equipped with high resolution collimators. SNs were identified intra-operatively using a handheld gamma detection probe (Navigator GPS-Tyco) and intra-operative lymphatic mapping with blue dye. After a resection of the SNs, a standard radical hysterectomy with pelvic and low para-aortic lymph node dissection was performed. Tumor characteristics were compared with sentinel node detection and with the histopathological and immunohistochemical results. RESULTS: The scintigraphy showed a focal uptake in 35 of the 37 patients. In all women one or more sentinel lymph nodes were identified intra-operatively. Of them, 24 patients had those located bilaterally. Histologically positive SNs were found in 5 women (13.5%). CONCLUSIONS: A combination pre-operatively administered radioactively labelled albumin with blue dye allows the successful detection of SN in patient with cervical cancer. This technique will result in a real advance in the less aggressive management of patients with early stage cervical cancer. Sentinel lymph node status may be representative of the pelvic lymph nodes status in cervical cancer and thus could provide important information for further treatment.

Adenocarcinoma↗
PubMed2002

[The value of colposcopy and computerised colposcopy in diagnosis and therapeutic management of CIN and early invasive cervical cancer in pregnant women].

OBJECTIVES: The aim of the study was determining the value of colposcopy, especially with computerised documentation of serial colposcopic pictures in diagnosis and therapeutic management of CIN and early cervical cancer in pregnant women. MATERIAL AND METHODS: Material consisted of 96 pregnant women aged 26-45 with CIN and Ca IA. Patients were diagnosed and treated between 1990-1999. There were performed colposcopic examinations, cytology and histological evaluation of colposcopy directed specimen. Lately colposcopic pictures were stored as graphic files by camera connected to PC. RESULTS: The positive correlation between colposcopy and histology was established in 86 women (89.9%) and between cytology and histology in 76 women (79.2%). Progression of CIN and Ca IA was noted in 8 women (8.3%). CONCLUSIONS: Colposcopy, especially computerised colposcopy enables cervical lesion evaluation in pregnant women and thus enables proper therapeutic management.

Colposcopy↗
PubMed2002

[Ovarian tumors in pregnancy in the material of the Department of Gynecology and Oncology Collegium Medicum of Jagiellonian University in Cracow].

OBJECTIVES: Although ovarian tumors are rare in pregnancy, they constitute a real diagnostic and therapeutic challenge. DESIGN: The aim of the study was to describe pathomorphological and clinical characteristic of ovarian tumor in pregnancy and to review the effects of diagnostics and treatment. MATERIALS AND METHODS: The study included 76 pregnant women treated due to ovarian tumors at Department of Gynaecology and Oncology, Collegium Medicum of Jagiellonian University. The age of patients range from 18 to 41 years (mean age 26 years). Diameter of each tumor was more then 5 cm. RESULTS: The symptoms of the tumors occurred in 25% of patients. Most of them were unilateral (96.05% cases). In 88.16% of cases the tumor diameter did not exceeded 10 cm. 71.05% of tumors were detected in first trimester of pregnancy. More then 50% of cystic tumors, that had not been surgically treated directly after detection disappeared during observation. Most common histological type of operated tumors were teratoma adultum (50%) and corpus luteum cyst. The malignant tumor of ovary was detected in 3.12% of cases. Spontaneous abortion after surgical treatment occurred in one patient after, emergency operation due to tumor torsion. CONCLUSIONS: Most of the ovarian tumors in pregnancy were benign. Incidence of ovarian malignancies in pregnancy did not exceed 4%. More then 50% of tumors that had not been removed directly after detection disappeared in the course of observation. Spontaneous abortion after operation rarely complicated surgical treatment.

Abortion, Spontaneous↗
PubMed2002

[Lymph node mapping and sentinel node detection in gynecological oncology].

The aim of this paper is the presentation of the latest opinions on the lymph node mapping and the sentinel node localization in the female genital organ neoplasm. The current strategies of lymph node resection in gynecologic oncology have been presented. The methods of lymph node staining and detection has been expounded as well. The paper also contains the results of sentinel node localization in the vulvar, cervical and endometrial cancers.

Coloring Agents↗