[Commemoration of the 25th anniversary of the Health Research Foundation].
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Academies and Institutes↗
Biomedical subjects
Antonio Campos Muñoz
Publications and source records attributed to Antonio Campos Muñoz.
[Artificial cornea, cell culture and tissue engineering].
The cornea, the fibrous transparent layer of the eye ball, is becoming a target of tissue engineering research because of the scarcity of donors. We report our experience in developing a mechanism to build a full-thickness corneal substitute using cell culture and protocols of tissue engineering. Isolation of keratocytes, epithelial and endothelial cells was accomplished by culturing denuded corneal stroma, limbal explant and enzymatically detached endothelium. Each type of cell was cultured in selective medium. Microanalytical quality control of cell viability was carried out. A multilayered cornea construct was built by sequential deposition of endothelium, keratocytes immersed a gel of human fibrin and epithelium in a manner that reproduce the normal histological structure.
Artificial Organs↗
[Mitochondrial apoptosis].
Silver staining methods, when selective, yield a high-contrast and high-resolution image in optical microscopy. A classical method for silver impregnation of mitochondria has been applied to liver tissue and reveals a marked heterogeneity among mitochondria in single cells. This heterogeneity can be detected in the optical microscope but is even more evident at the ultrastructural level. The differences in staining intensity may reflect different stages in the mitochondrial life cycle. The most lightly stained mitochondria and the unstained mitochondria also observed with Electron microscopy may represent the earliest phase of the mitochondrial life cycle. On the other hand, the progressively darker staining and final appearance of shrunken mitochondria may reflect the autodissolution process of these organelles. Such a process of mitochondrial apoptosis might make sense as a preventive pathway for elimination of damaged mitochondria before cytC or other proapoptotic factors could be liberated. This method reveals a marker of mitochondrial age.
Apoptosis↗
[Financing sources in urologic clinical research].
One of the most important challenges of research in the health sciences is to approximate basic, clinical, and epidemiological research, and to convert hospitals and outpatient clinics in nuclei of vanguard and innovation in this field. It is not possible to develop quality research without a quality clinical practice and vice versa. Because of this, all investment efforts made in this field will always be actions that, immediately or on the long-term, will finally result in an improvement in the quality of patient care for the citizens, and in greater development impulse and transfer of great amount of technology and innovation that is inexorably emerging in this field. Next there is a summary of the National Plan of Scientific Research, Development and Technological Innovation 2000-2003 and the role of the Institute of Health Carlos III in the promotion of the health science's research in Spain.
Biomedical Research↗