Biomedical subjects

Aung Si

Publications and source records attributed to Aung Si.

5 recordsLinked to original sources

PubMed2006

Effects of caffeine on olfactory and visual learning in the honey bee (Apis mellifera).

Although caffeine is known to improve alertness and arousal in humans and other mammals, its impacts on specific behaviours, including complex cognitive processes, remain controversial. We reasoned that the availability of an easily manipulable, but behaviourally complex invertebrate organism with a simpler nervous system would be beneficial to this field of research. We used a popular behavioural model, the honeybee, to evaluate the effects of caffeine on (1) the development of olfactory learning and (2) the performance in complex learning paradigms, including a 'delayed-match-to-sample' task and visual associative learning. To evaluate the efficacy of caffeine treatment, a variety of doses (0.4-400 ng/1 mg of body mass) were applied topically to tethered individuals. Behavioural testing was performed with either tethered or free-flying adult honeybees. We show that caffeine has marked cognitive effects in this species. In young honeybees, it reduces the age at which restrained individuals are able to learn an olfactory associative task, whereas in older, free-flying bees, caffeine improves both motivation and cognitive performance in complex learning tasks. Our results suggest that the honeybee model may be useful in explaining caffeine-related behavioural changes not only in this species, but also in mammalian systems.

Animals↗

PubMed2005

Visual working memory in decision making by honey bees.

The robustness and plasticity of working memory were investigated in honey bees by using a delayed matching-to-sample (DMTS) paradigm. The findings are summarized as follows: first, performance in the DMTS task decreases as the duration between the presentation of the sample stimulus and the presentation of the comparison stimuli is increased. This decrease is well approximated by an exponential decay function. Performance is significantly better than random-choice level even at delays as long as 5 sec and is reduced to random-choice levels at an average delay time of 8.68 +/- 0.06 sec. Second, when the DMTS task involves two samples (one relevant, the other irrelevant), bees can be trained to learn to use the relevant sample to perform the task if (i) the relevant sample is always at a fixed position, or (ii) the relevant sample always has the same place in the sequence of presentation (always first or always second). Bees that have learned to use the relevant sample and to ignore the irrelevant sample can generalize this learning, and apply it to novel sets of sample and comparison stimuli that they have never previously encountered. The findings point to a remarkably robust, and yet plastic, working memory in the honey bee.

Animals↗

PubMed2004

Honeybee odometry: performance in varying natural terrain.

Recent studies have shown that honeybees flying through short, narrow tunnels with visually textured walls perform waggle dances that indicate a much greater flight distance than that actually flown. These studies suggest that the bee's "odometer" is driven by the optic flow (image motion) that is experienced during flight. One might therefore expect that, when bees fly to a food source through a varying outdoor landscape, their waggle dances would depend upon the nature of the terrain experienced en route. We trained honeybees to visit feeders positioned along two routes, each 580 m long. One route was exclusively over land. The other was initially over land, then over water and, finally, again over land. Flight over water resulted in a significantly flatter slope of the waggle-duration versus distance regression, compared to flight over land. The mean visual contrast of the scenes was significantly greater over land than over water. The results reveal that, in outdoor flight, the honeybee's odometer does not run at a constant rate; rather, the rate depends upon the properties of the terrain. The bee's perception of distance flown is therefore not absolute, but scene-dependent. These findings raise important and interesting questions about how these animals navigate reliably.

Animal Communication↗

PubMed2004

Effects of NMDA receptor antagonists on olfactory learning and memory in the honeybee (Apis mellifera).

In contrast to vertebrates the involvement of glutamate and N-methyl-D-aspartate (NMDA) receptors in brain functions in insects is both poorly understood and somewhat controversial. Here, we have examined the behavioural effects of two noncompetitive NMDA receptor antagonists, memantine (low affinity) and MK-801 (high affinity), on learning and memory in honeybees (Apis mellifera) using the olfactory conditioning of the proboscis extension reflex (PER). We induced memory deficit by injecting harnessed individuals with a glutamate transporter inhibitor, L-trans-2,4-PDC (L-trans-2,4-pyrrolidine dicarboxylate), that impairs long-term (24 h), but not short-term (1 h), memory in honeybees. We show that L-trans-2,4-PDC-induced amnesia is 'rescued' by memantine injected either before training, or before testing, suggesting that memantine restores memory recall rather than memory formation or storage. When injected alone memantine has a mild facilitating effect on memory. The effects of MK-801 are similar to those of L-trans-2,4-PDC. Both pretraining and pretesting injections lead to an impairment of long-term (24 h) memory, but have no effect on short-term (1 h) memory of an olfactory task. The implications of our results for memory processes in the honeybee are discussed.

Animals↗

PubMed2003

Honeybee navigation: properties of the visually driven 'odometer'.

Recent work has revealed that honeybees determine distance flown by gauging the extent to which the image of the environment moves in the eye as they fly toward their destination. Here we examine the properties of this visually driven 'odometer', by training bees to fly to a feeder in a tunnel lined with a range of different visual patterns, and analysing their dances when they return to the hive. We find that the odometric signal is relatively unaffected by variations in the contrast and spatial frequency content of the patterns. Furthermore, a strong signal is generated even when the walls or the floor of the tunnel provide only weak optic-flow cues. Thus, distance flown is measured by a visually driven odometer that is surprisingly robust to variations in the texture or sparseness of the visual environment through which the bee flies.

Animals↗