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Biomedical subjects

Avi Wiener

Publications and source records attributed to Avi Wiener.

2 recordsLinked to original sources

Objective measurement of knee extension force based on computer adaptive testing.

False impairment is encountered when tested subjects either unintentionally or deliberately put an artificial upper limit on their force, in which case their true capacity cannot be disclosed in a straight forward measurement. The aim of this study was to develop a computer adaptive testing (CAT) system for directing subjects into generating greater forces than they intended. The system was tested on eleven cooperative female subjects who volunteered to take part in this study. The CAT consisted of interactive testing cycles, each containing a series of isometric tasks of differing intensities. While fulfilling these tasks, the tested subjects were asked to take care not to exceed a self-selected upper force limit (F(ssl)) that they were previously trained to memorize (order of 40% of the maximal voluntary contraction). Visual feedback, displaying the applied force exertions, was provided to the tested subjects but was modified by re-scaling the display in an un-anticipated manner. To confirm the subject's ability to remember her F(ssl), repeatability of joint memory was tested one week after the CAT. The CAT results were successful in causing ten out of the eleven tested participants to exert a higher force than they intended to. Additionally, the CAT algorithm caused a statistically significant higher force than the repeatability test. These results demonstrate the potential of CAT methods in improving the clinical evaluation of muscle strength, particularly in those cases where the subject's cooperation is not sufficient.

Adult↗

Monitoring of occupational exposure to methylene chloride: sampling protocol and stability of urine samples.

A sampling protocol for biomonitoring of the volatile solvent methylene chloride (MeCl(2)) by analysis of urine from exposed workers was established. Storage temperature, sample volume in headspace vial (HSV), and time to sealing HSV on determination of MeCl(2) in urine were evaluated. MeCl(2) was analyzed by a solid-phase microextraction technique combined with gas chromatography. Volume of urine in HSV has no effect on MeCl(2) analysis. Delays of 30 and 60 min from collection of urine until sealing the HSV caused 14.47 +/- 6.98% and 26.17 +/- 9.57% decreases from baseline concentration, respectively. MeCl(2) concentration in spiked urine samples stored in sealed HSVs decreased on day 2 and then remained stable for 2 weeks. Refrigeration did not improve recovery although it seems to be associated with less variability. MeCl(2) in urine samples of seven exposed workers was in the range of 0.02-0.06 mg/L. Sampling of MeCl(2)-containing urine should include collection of urine in closed plastic bottles, transfer to HSV within 15 min, sealing and clamping of HSV within 15 s, and storage of HSV in refrigeration until analysis, but no longer than 2 weeks. Standard samples should be prepared on the day of test sample collection and handled under the same conditions.

Adult↗