PubMed Health⌕ Search

Biomedical subjects

B A Botros

Publications and source records attributed to B A Botros.

At least 37 records · Page 2Linked to original sources
PubMed1987

Arboviral causes of non-specific fever and myalgia in a fever hospital patient population in Cairo, Egypt.

Fever and myalgia are non-specific clinical manifestations of illness which commonly occur in patients with arboviral disease. In Egypt, such illness is often mis-diagnosed as "influenza". To determine arboviral aetiology in patients admitted with fever and myalgia, acute and convalescent sera samples were obtained from 55 patients admitted with these clinical manifestations to the Imbaba Fever Hospital, Giza, Egypt, during October and November 1984. Based on viral isolation, and a comparison of acute and convalescent sera, 4 patients (7%) had acute arboviral infections. Haemagglutination inhibition and indirect immunofluorescence tests showed that one had West Nile virus (WNV) infection, 2 had sandfly fever virus-Naples (SFN), and 1 had sandfly fever virus-Sicilian (SFS) infection. SFN was isolated from the acute serum sample of 1 of the 2 patients with SFN infection.

Adolescent↗
PubMed1986

Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta.

To determine the accuracy of serological methods in detecting Rift Valley fever (RVF) viral antibodies, we examined serum samples obtained from 418 sheep in the Nile Delta by using five tests. The plaque reduction neutralization test (PRNT) was considered the standard serological method against which the four other tests were compared. Twenty-four serum samples had RVF viral antibodies detected by PRNT. Hemagglutination inhibition and enzyme-linked immunosorbent assay antibodies to RVF virus were also present in the same 24 serum samples. Indirect immunofluorescence was less sensitive in comparison with PRNT, and complement fixation was the least sensitive. These results extend observations made with laboratory animals to a large field-collected group of Egyptian sheep.

Animals↗
PubMed1982

Technique for liver biopsy in the dromedary camel.

A technique for needle liver biopsy in camels was developed. Xylazine, 15 mg/100 kg intravenously, was used for sedation and analgesia. A 1.2 mm X 7 cm Menghini needle was introduced anteroventrally through the ninth right intercostal space, 1 cm caudal to the ninth rib and 2 cm dorsal to the costal cartilage. The needle was directed toward the contralateral olecranon with the camel in sternal recumbency. A core of liver tissue between 1 and 2 cm long was obtained from 99 of 120 attempts in 23 animals. There were no adverse effects from the procedure and histological preservation of the tissue was excellent.

Animals↗
PubMed1979

A study to evaluate non-fatal rabies in animals.

Brains and salivary glands of stray dogs, cats and wild mammals were examined for latent rabies virus. Sera of these animals were tested for rabits neutralizing antibody. Dogs and rodents of the gerbil species were experimentally infected with rabies viruses tryping to produce nonfatal rabies. The following conclusions are made: 1. Naturally occurring nonfatal rabies infection evidenced by recovery of the virus was not present in the stray dogs examined and could not be produced experimentally. 2. Among wild gerbils showing no signs of disease, recovery of (latent) virus was possible but this condition could not be reproduced experimentally. However they could have been in the incubation stage. 3. Development of rabies neutralizing antibody did not occur naturally in wild rodents, but occurred in dogs. Antibody to rabies was detected in surviving gerbils after experimental infection. The antibody probably develops subsequent to sublinical nonfatal infection. 4. Non-fatal infection, indicated either by recovery of rabies virus or detection of neutralizing antibody, was not detected among the examined cats.

Animals↗
PubMed1978

Equine leptospirosis with some clinical observations.

In a serologic survey on equine leptospirosis in Egypt, the following incidences of leptospiral serosensitivity were found: 1. Hospitalised horses 65/113 (57.5 %). 2. Hospitalised donkeys 90/125 (72.0 %). 3. Apparently healthy horses 21/72 (29.1 %). Sera of these animals were mostly reacting to serotypes butembo, pomona, icterohemorragiae, and grippotyphosa. Equine in Egypt are close animals to humans and may constitute a potential source of leptospiral infection. From the clinical point of view, it is very possible that ocular, hoof lesions and icterus in equines would be expected with leptospiral titres.

Animals↗
PubMed1977

Rabies in the Arab Republic of Egypt: III. Enzootic rabies in wildlife.

Five hundred and thirty-two rodents (10 species) and 112 other wild mammals (six species) were examined for rabies infections in Egypt. Rabies virus was isolated from the brains of three (1-2%) of 249 Gerbils, Gerbillus gerbillus and one (1-8%) of 56 foxes, Vulpes species. In neutralization tests these isolates were found to be identical to classical rabies virus. Significant numbers of rodent and other wild mammal species need to be examined before they may be excluded as possible reservoirs of rabies virus in Egypt. Based on initial findings it may be wise to reevaluate antirabies treatment for rodent bites occurring within the Arab Republic of Egypt.

Animals↗
PubMed1976

Human histoplasmosis in Egypt-a preliminary serologic survey.

Thirteen (3%) of 441 patients had histoplasma antibodies in their sera when examined by the complement fixation test. The serologically histoplama positive individuals were all from a group of 266 patients complaining of chest disorders and from a sub-group of 147 such patients with positive findings on chest x-ray. There were no positives from a group of 175 patients complaining of fevers of unknown origin. The possibility of misdiagnosing lesions which may be due to histoplasmosis as being tuberculous must be considered indifferential diagnosis of pulmonary lesions.

Complement Fixation Tests↗
PubMed1975

Some observations on experimentally induced infection of dogs with Babesia gibsoni.

Splenectomized andnonsplenectomized dogs were experimentally infected with Babesia gibsoni. Infectivity of parasites was retained for 1 month in samples of blood kept at 4 C in a mixture with Alsever's, acid-citrate-dextrose (ACD), or ammonium-potassium oxalate solutions. When samples were slowly frozen to -70 C in a mixture with citrate solution, the parasites remained infective for 4 months. The average prepatent period was 3.3 days in splenectomized dogs and 4 days in nonsplenectomized dogs. Clinical signs were mild fever and anemia in nonsplenectomized dogs and fever, anemia, icterus, and rarely, hemoglobinuria in splenectomized dogs. Blood packed cell volume (PCV) decreased to as little as 11%, and total bilirubin increased to as great as 0.85 mg/dl. Latent parasitemia was still detectable in some dogs 135 days after the initial parasitemia. Gross pathologic changes mainly involved liver and spleen. Hepatic degeneration was always present.

Age Factors↗
PubMed1975

Leptospirosis in Ethiopia: a serological survey in domestic and wild animals.

Seven hundred and fifty-eight serum samples from domestic and wild animals in Ethiopia were tested for leptospiral antibodies by the microscopic agglutination test. The following percentages of seropositivity were obtained: horse (91.3 per cent), cow (70.7 per cent), pig (57.1 per cent), goat (47.3 per cent), sheep (43.4 per cent), camel (15.4 per cent), and dog (8.3 per cent). All 54 samples from birds and wild animals were seronegative. Most of the positive sera exhibited reaction to more than one serotype and antibodies to serotype butembo were predominant in sera of the majority of the examined species. However, grippotyphosa, rather than butembo was the predominant reacting serotype in camels and dogs. It is concluded that leptospirosis is of significant prevalence in domestic animals in Ethiopia and may constitute an occupational health hazard to man.

Agglutination Tests↗