Biomedical subjects
Publications and source records attributed to B A Fikhte.
A method is proposed for evaluating the intracellular quantity of storage substances (lipids and glycogen) in dormant cells, on the basis of a stereological analysis of the electron-microscopic image of cross-sectioned freeze-etched cells.
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Vesicular bodies are observed during enzymatic degradation of the yeast cell wall and in the course of wall regeneration. In both cases various types of vesicles are formed and exocytated by different mechanisms. They are characterized cytochemically and their possible role in cell wall regeneration is discussed.
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The elasticity modulus of Escherichia coli cell walls was found to change in the course of growth of the culture. It decreased in the middle of the exponential phase, apparently as a result of intensification of biosynthetic and related to them transport processes at this period. If the cells were treated with EDTA, their elasticity modulus was lower, by an order of magnitude, than that of the control cell walls. If the cells were treated with lysozyme, the elasticity modulus of their walls decreased by three orders of magnitude. The elasticity modulus of cell walls was less in gram-positive rods than in E. coli. The elasticity modulus of spherical cells cannot be determined using this technique.
The interaction of a parasite with a host was studied in the two-membered bacterial system, Bdellovibrio bacteriovorus 109D and Escherichia coli B, immobilised in polyacrylamide gel (PAAG). The parasite localised inside the host cells was found to be more resistant to the toxic action of PAAG components than free B. bacteriovorus. The latter lost its mobility and was inactivated in the matrix of the carrier whereas the intracellular parasite had a normal cycle of development in the periplasm of the infected cells. The dynamics of B. bacteriovorus and E. coli incidence in the liquid phase and in PAAG granules was studied while the immobilised system was incubated. The interaction in the immobilised system could be intensified by growing more bacterial host cells in PAAG particles. The immobilisation was shown to favour the survival of the parasite and the host in the two-membered system.
The effect of the bacteriolytic preparation "Lysoamidase" on Staphylococcus aureus 299 P was studied. The maximum activity of the preparation was observed at pH 8.0 ionic strength 0.01-0.02 M and 50-60 degrees of the incubation medium. The electron microscopic examination revealed that "Lysoamidase" hydrolyzed the cell wall in one or several points with the following osmotic shock and extrusion of the cytoplasm. In an isotonic solution (1 M sucrose) "Lysoamidase" caused protoplast formation.