Biomedical subjects

B A Nikulin

Publications and source records attributed to B A Nikulin.

3 recordsLinked to original sources

PubMed1997

[Proliferative activity of lymphocytes in a culture with phytohemagglutinin and immunity parameters in acute pneumonia].

Proliferative activity of lymphocytes in a culture was assessed by flow cytometry and lymphocyte blast transformation test (LBTT) with the proliferative pool of cells (S+G2+M) from 150 patients with acute pneumonia, 30 of these with the mild form, 97 with medium-severe, and 27 with grave form; in 43 patients the disease duration was over 30 days (protracted pneumonia). In addition, levels of R.protein, homoreactant, immunoglobulins, complement components, T, B, and active T lymphocytes, phagocytosis, and granulocyte oxidative metabolism were assessed. LBTT values were in inverse relationship with the disease severity. Correlations between the studied immunity parameters were analyzed. The findings indicate that LBTT is an informative method for assessing the immune reactivity and may serve for predicting the disease course and for the differential diagnosis of pulmonary diseases.

Acute Disease↗

PubMed1994

[The procedures for using immunological study methods in different diseases].

The article deals with the basic principles of application of immunological methods for the diagnosis of the immune system disorders. A two-staged method of laboratory tests for the immune status evaluation was accompanied with a number of immunological methods which differed depending on the proper status of the immune system. The author stresses the clinical significance of the methods of the immune status evaluation and also gives the basic immunological criteria for the immunomodulatory therapy.

Autoimmune Diseases↗

PubMed1989

[Flow cytofluorimetric analysis of the proliferative activity of lymphocytes in a culture with phytohemagglutinin].

Phytohemagglutinin (PHA)-effected lymphocyte blastogenesis test (LBGT) in culture has been assessed by flow cytofluorometry in 63 normal subjects and in 160 patients suffering from various pulmonary diseases. An algorithm for the estimation of cellular distribution in accordance with the mitotic cycle stages is suggested, and a computer program for the analysis of the results, based on the mitotic cycle stages, has been compiled. The lymphocyte proliferative activities have been estimated with various PHA doses. The findings have been in good correlation with the results of radionuclide studies (3H thymidine label). The described approach to LBGT assessment yields reliable results and may be used in diagnostic studies.

Adult↗