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Biomedical subjects

B Acosta

Publications and source records attributed to B Acosta.

36 records · Page 2Linked to original sources
PubMed1999

[Neonatal colonization by Ureaplasma urealyticum and the development of bronchopulmonary dysplasia].

OBJECTIVE: The study purpose was to investigate the association between neonatal Ureaplasma urealyticum isolation, and bronchopulmonary dysplasia (BDP), in infants who had birth weights < 1,500 g, admitted in the Pediatric Intensive Care Unit, La Fe Hospital, Valencia, Spain. METHODS: A cohort study was designed, since March 1996 until April 1998, with a cohort of exposed and no exposed, determined by isolation of U. urealyticum in tracheal aspirate or pharyngeal cultures. 137 infants were admitted to the intensive care unit in this period, and 101 of them were enrolled in this study. RESULTS: U. urealyticum was isolated in 27 (26.7%) patients. The infants with U. urealyticum positive culture had a significantly lower gestational age (mean 28.1, SD 2.4 vs 29.2, SD 2.4; p = 0.048) than negative culture infants. BDP occurred in 30 infants (29.7%), and was significantly associated with decreasing gestational age (mean 26.9, SD 1.7 vs 29.7, SD 2.2; p < 0.001) and lower birth weight (mean 965.8, SD 166.7 vs 1121.4, SD 232.1; p < 0.001). However, after correction for gestational age by logistic regression analysis, DBP was significantly related to decreasing gestational age, but not to the presence of a positive U. urealyticum culture. In the cohort of U. urealyticum positive infants, BPD occurred in 9 (33%) vs 21 (28%) in the not colonized cohort. The odds ratio associated with colonization was 1.3 (CI 95% 0.5-3.2; p = 0.63). CONCLUSIONS: BPD was significantly associated with decreasing gestational age, independently of birth weight and U. urealyticum colonization.

Bronchopulmonary Dysplasia↗
PubMed1997

IgG concentration in mammary secretions of goats throughout lactation in healthy and coagulase-negative staphylococci infected udders.

Radial immunodiffusion was used to determine immunoglobulin (Ig)-G concentrations in 16 mammary secretions from uninfected udder halves and in 10-14 secretions from halves subclinically infected with coagulase-negative staphylococci in goats throughout lactation. IgG concentrations in samples from uninfected halves decreased rapidly during the first week after parturition (P < 0.001 ), henceforth falling slowly up to 30 d post-partum (P < 0.001). From 30 d post-partum to 180 d of lactation, IgG concentration showed a continuous decline to the end of the experiment but these changes were not statistically significant until 150 d after kidding (P < 0.01). IgG concentrations in lacteal secretions of halves harbouring coagulase-negative staphylococci showed a similar pattern, from the first month of lactation, to that observed in healthy udder halves, but concentrations were always lower. These differences were significant from 2 months after parturition (P < 0.001). IgG concentrations were compared with somatic cell counts obtained using an automatic counter (Fossomatic).

Animals↗
PubMed1996

[Enterovirus meningoencephalitis in the last 5 years].

The results of the study of Enterovirus as viral meningoencephalitis producing agents, carried out from 1990 to 1994, are described, 546 feces samples, 95 cerebrospinal fluids and 1,058 matched sera were studied and obtained from 1,388 patients clinically diagnosed with this disease. Samples for viral isolation were inoculated into two different cellular systems. The highest number of isolation was found in diploid cells from human fibroblast. Antibody determinations were carried out by a neutralization test (micromethod) with 11 Enterovirus antigens (Echo 4, 6, 9, 11 and 30; and Coxsackie B1, 2, 3, 4, 5 and 6) and in epidemic periods with the isolated virus. During the years under study, 2 epidemic outbreaks took place: on caused by Coxsackie A9 (1990-1991) and the other one by Echo 30 (1994). A greater positivity to Echo 6 and 11 was found among the matched sera.

Antibodies, Viral↗
PubMed1994

Polyarthritis in kids associated with Mycoplasma putrefaciens.

Fifteen two- to three-week-old kids, fed artificially with goats' milk from a dairy, were found to have polyarthritis. The most affected joints were the carpals, either unilaterally or bilaterally, and in some cases the tarsal joints. Mycoplasma putrefaciens was isolated from the joints which showed an acute fibrinopurulent arthritis. No clinical mastitis was detected in the dairy.

Animals↗
PubMed1991

[Detection of cytomegalovirus in urine by the shell-vial technique: usefulness of diluting the sample].

To assess the toxicity of urine samples on human fibroblast cell monolayer using Shell-vial technique for cytomegalovirus antigen detection. A total of 227 urine samples were processed, inoculated on Shell-vial simultaneously, being either diluted (1:1) and non diluted in transport media. After 24-48 hours incubation, the fibroblast cells monolayer was stained using fluorescein-marked monoclonal anti-cytomegalovirus antibodies. Cytomegalovirus was detected in 9.6% of samples inoculated with diluted urine, and in 5.2% of samples inoculated with non-diluted urine. Destruction of monolayer cells was seen in 13.2 and 22.4, respectively. With Shell-vial technique for detecting cytomegalovirus in urine samples, a high number of positive results was obtained using diluted urine. Also a significant minor destruction of cells monolayer was observed with the same inoculum.

Antibodies, Monoclonal↗
PubMed1983

Nursing enhances the negative effect of estrogen on LH release in the cow.

Twenty-three crossbred beef cows between 4 and 5 yr of age were assigned at random to one of six treatments: (1) ovariectomized 4 d postpartum (OVX) with early weaning of calves 21 d postpartum (OVX-EW; n = 4), (2) OVX-EW and 17 beta-estradiol implants (OVX-E2-EW; n = 4), (3) OVX and normal nursing by calves throughout the experiment (OVX-NN; n = 3), (4) OVX-NN and 17 beta-estradiol implants (OVX-E2-NN; n = 4), (5) intact cows and early weaning of calves 21 d postpartum (EW), (6) intact cows and normal nursed (NN). Blood was collected at 15-min intervals over a 4-h period once weekly during the 12-wk postpartum period in the OVX cows. Early weaned intact cows exhibited estrus 23 d sooner (P less than .05) than normally nursed cows. A hormone level for each cow at each week was determined from the mean of the 17 samples collected over the 4 h period each week. There were no significant changes due to E2 treatment, for concentrations of LH, FSH or number of pulses during wk 1 through 3. However, during wk 4 through 12 the linear and quadratic contrasts of wk X estrogen X nursing were significant for serum LH, indicating there was no difference between the treatments for EW and NN without E2 but there was a large difference in the presence of E2. During nursing E2 suppressed serum LH below that of nonestrogen-treated cows while after weaning E2 stimulated LH release above that of nonestrogen-treated cows.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Population Groups↗
PubMedDate not supplied

[Prevalence of antibodies against herpes simplex virus, Epstein-Barr virus and cytomegalovirus in a group of patients after hemodialysis].

Infections from herpes virus play a key role in post-transplantation pathology, so it is indispensable to characterize the group of would-be renal transplant recipients. IgG antibody titers were detected by indirect immunofluorescence to Herpes simplex virus, Epstein Barr virus (EBV) and cytomegalovirus (CMV) in 100 patients treated with hemodialysis in the Nephrology Institute. The prevalence of antibodies to cytomegalovirus and to Herpes simplex and Epstein Barr viruses was 100% and 95% respectively. Average geometric titers (AGT) of antibodies to CMV were significantly higher. There was no correlation between AGT and sex, age, time of disease remission, number of blood transfusions and number of transplants.

Adult↗
PubMedDate not supplied

[Prolonged total parenteral nutrition in surgery. A case report of severe malnutrition].

We present a 15-years-old female patient severely malnourished due to complications of abdominal surgery, who received total parenteral nutrition during 118 days. The clinical and laboratory parameters are analyzed and a photographic sequence is presented. During the treatment period nitrogen balance was positive and her weight increased in 50%.

Acute Disease↗
PubMedDate not supplied

[Vascular compression of the duodenum].

The acute vascular compression of the duodenum is a well-recognized clinical entity, characterized by recurrent vomiting, abdominal distention, weight loss, post prandial distress. The cause of compression is considered to be effect produced as a result of the angle formed by the superior mesenteric vessels and sometimes by one of its first two branches, and vertebrae and paravertebral muscles, when the angle between superior mesenteric vessels and the aorta it's lower than 18 degrees we can saw this syndrome. The duodenojejunostomy is the best treatment, as well as in our patient.

Adolescent↗
PubMedDate not supplied

[Ligation and sclerosis technique combined with electrocoagulation resection of internal hemorrhoids].

We review 1331 patients who were treated by sclerosis and rubber band ligation, stepped binding, simultaneous binding and sclerosis with electrocoagulation resection as the main hemorrhoidal bundle treatment, from July 1977 through July 1990, was performed. The benefits on symptoms, complications, and final outcome were analyzed. Binding of only one hemorrhoidal bundle with sclerosis of the other two in the 74.9%, binding of two bundles with sclerosis of the other in the 13.7% and simultaneously binding of the three bundles in the 4.8% was performed. Binding, sclerosis and electrocoagulation resection of three bundles was also performed in the 6.45%.

Adolescent↗
PubMedDate not supplied

[Heterotopic pancreas].

Heterotopic pancreas is a rare entity, usually asymptomatic, and found incidentally during laparotomy, when symptomatic it is for some complication, gastric obstruction, gastrointestinal hemorrhage, Meckel's diverticulum, obstructive jaundice. We report four patients, with this diagnostic and make a bibliographic review of this entity.

Adolescent↗
PubMedDate not supplied

[Aeromonas spp. isolated from human feces. Species and pathogenicity factors].

The role of Aeromonas genus as a primary intestinal pathogen is still a matter of controversy. We have studied the isolation of Aeromonas spp. from human stools samples, looking for the production of pathogenic factors and its relationship with different species. From a total of 471 strains isolated, 241 were A. caviae, 127 A. sobria, 89 A. hydrophila and 14 non-typable strains. The pathogenic factors production was studied in a bacteria-free filtrate from a 18 hours trypticase-soy broth culture. Enterotoxin production was assessed by suckling-mice test, cytotoxin production by using Hela cells monolayer and hemolysin production by double dilution from the filtrate, plated in microdilution plates, against rabbit red-blood cells. Enterotoxin production test was positive for A. sobria, A. hydrophila and A. caviae in 81.1%, 62.9% and 7.0% of strains respectively. Cytotoxin production was also positive for these species in 96.6%, 76.4% and 4.1%, and hemolysin production was positive in 95.3%, 70.7% and 2.4% of the strains respectively.

Aeromonas↗
PubMedDate not supplied

[3 cases of hemobilia].

Three cases of Hemobilia in relation with complications associated to: Hepatic biopsy, retrieval of T-tube drainage and liver trauma are presented. A review of the clinical picture, diagnostic procedures and surgical treatment in every case is analized. General revision of this entity is made and according with our experience suggest some conclusions.

Adult↗
PubMedDate not supplied

Septicemia associated with Hafnia alvei in laying hens.

In the present work Hafnia alvei was isolated from laying hens displaying a reduction in egg production, loss of appetite, opisthotonus, and death. Multifocal necrotizing hepatitis and splenitis were the most prominent lesions. The organism was identified microbiologically. Laying hens were experimentally inoculated by the oral and intraperitoneal route to show the pathogenicity of the organism. A very similar clinicopathologic effect resulted from this trial. Several experimentally infected laying hens died due to septicemia. We conclude that H. alvei may cause a septicemia similar to that reportedly caused by Salmonella spp. in avian species.

Animals↗