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Biomedical subjects

B Albini

Publications and source records attributed to B Albini.

90 records · Page 5Linked to original sources
PubMed1975

Antigenic surface determinants of chicken lymphoid cells. II. Selective in vivo and in vitro activity of anti-bursa and anti-thymus sera.

Appropriately absorbed turkey antisera to antigenic surface determinants of chicken bursa (ABS) or thymus cells (ATS) were assessed for their selective immunosuppressive activy in vitro and in vivo. The intraperitoneal injection of ABS or ATS into 2-3-week-old normal white Leghorn chickens entailed a significant depletion of B or T cells respectively from spleen and peripheral blood, while bursa and thymus themselves remained unaffected. The potential of this 'peripheral serological bursectomy and thymectomy' paralleled that found after the conventional surgical procedures with subsequent sublethal irradiation. The mean survival time of skin allografts from donors of genotype B4B4 onto B8B8 recipients was significantly prolonged by treatment with ATS (29 plus or minus 12 days) as compared to untreated (14 plus or minus 2 days), normal turkey serum (12 plus or minus 3 days) or ABS-injected (13 plus or minus 2 days) groups. This selective suppression of a T cell-dependent immune reaction by ATS was also confirmed in vitro by its inhibitory action on the graft-versus-host reactivity of adult peripheral blood lymphocytes in the chorioallantoic membrane assay, where normal turkey serum and ABS were again ineffective. Thus, ABS or ATS produced in avian species may serve not only to delineate B and T cells in vitro, but can also be used for selective manipulation of immune reactions of the chicken in vivo.

Animals↗
PubMedDate not supplied

Effect of alcohol on spleen cells and their functions in C57BL/6 mice.

Spleen cells from C57BL/6 mice maintained on alcohol containing liquid diet for two weeks were evaluated for different immune functions. On an average, 22% fewer spleen cells were recovered from alcohol-fed mice when compared to cells from control animals. In alcohol-fed mice, the relative frequency of B cells increased, whereas total T cells including CD4+ cells decreased significantly. Alcoholic mice, when challenged with poly(rI) poly(rC), produced significantly less interferon than control mice. In vitro production of interferon alpha and gamma by the spleen cells of alcoholic mice was reduced by 67-90%. No significant differences were seen in the level of natural killer cell activity in spleen cells of control and alcoholic mice. These results suggest that chronic alcohol intake can result in not only changes in the number of immune cells, but more importantly affect their biological functions such as their ability to produce interferons.

Alcohol Drinking↗
PubMedDate not supplied

Alteration of immune response induced by chronic intrarectal insemination in rabbits.

Three groups of adult male Nya:(FG) rabbits were treated intrarectally, 3 times a week for 7 months, with 1 ml of fresh homologous semen, 20 ml of colonic enema, or 20 ml of enema followed by 1 ml of previously frozen semen. A control group was untreated. The results of several in vitro tests of humoral and cellular response showed that chronic intrarectal insemination leads to an abrogation of the immune response, expressed differently in the two groups of rabbits receiving semen. A consistently depressed blastogenic stimulation in response to T-cell mitogens was observed only in the animals receiving fresh semen. Neither group produced antilymphocyte antibodies. Anti-seminal-fluid and antispermatozoa antibodies were detected after 3 months of treatment in two animals receiving enema and semen and one animal receiving fresh semen. Circulating immune complexes were detected after 2 months of treatment in animals receiving enema and semen. These immune complexes contained predominantly IgM and were specific for seminal antigens. Assay of the cellular response to a T-cell-dependent antigen showed, in the animals receiving enema and semen, a pronounced decrease in the indirect IgA and IgG plaque-forming cells response with practically no effect on the IgM response. These results, taken together with those of the preceding paper, suggest that in intrarectally inseminated rabbits the functional capabilities of the immune system may deteriorate without histologic or morphologic changes in the anorectal mucosa of relevant lymphoid tissues.

Animals↗
PubMedDate not supplied

Synthesis of intestinal basement membrane.

The rat small intestinal epithelial cell (enterocyte) has an average life span of 48h. Undifferentiated stem cells in the lower crypt region undergo division and differentiation into enterocytes as the cell moves on a basement membrane up the villus to reach the tip and be extruded into the lumen. The mechanism of this movement and relationship to basement membrane synthesis and/or renewal is unknown. This problem is discussed in regard to our findings suggesting that the major contribution to basement membrane synthesis and renewal may not be from the enterocyte.

Animals↗
PubMedDate not supplied

Experimental immune complex disease of the intestine.

The effect of immune complex deposition in intestine was studied in a mercuric chloride-induced experimental autoimmune disease model in the Brown Norway rat. Whereas deposition of autoantibodies in a linear pattern along the basement membrane did not lead to any functional changes, presence of immune complexes along the intestinal basement membrane, occurring after week 35 of the experiment, was associated with intestinal protein loss.

Animals↗