Biomedical subjects

B Asman

Publications and source records attributed to B Asman.

63 records · Page 4Linked to original sources

PubMed1992

Granulocyte elastase in gingival crevicular fluid. A possible discriminator between gingivitis and periodontitis.

The granulocyte elastase activity and the immuno-reactive (antigenic) granulocyte elastase of gingival crevicular fluid (GCF) were studied in 16 periodontitis patients and in 10 gingivitis patients. The elastase activity was measured with a low molecular weight substrate specific for granulocyte elastase. The antigenic elastase was determined with specific antibodies against granulocyte elastase. Intracrevicular sampling of GCF with paper strips for 30 s seemed to provide representative values of elastase. The elastase activity correlated with probing depth and attachment loss and appeared to be a measure of the degree of tissue destruction. Antigenic elastase represents the number of granulocytes in GCF and should thus be related to the degree of inflammation. The periodontitis patients and the gingivitis patients both had a similar degree of inflammation as measured by antigenic elastase per microliter GCF and gingival index. The elastase activity per microliter GCF, however, was higher in the periodontitis group. Elevated granulocyte elastase activity in GCF seems to be independent of inflammation and could thus be an indicator of patients at risk for periodontitis.

Adult↗

PubMed1988

Collagen degradation by experimentally-induced subcutaneous granulation tissue in the rat.

Granulation tissue provoked by implantation of cellulose sponge or a nylon bag degraded a powder of homologous collagen. This activity was located in a non-filtrable tissue fraction and was associated with the appearance of mononuclear and histiocytic giant cells, following an early polymorphonuclear-cell infiltration. The activity could be manipulated experimentally, indicating that this model can be used in investigations of inflammation-induced collagen degradation.

Animals↗

PubMed1988

Peripheral PMN cells in juvenile periodontitis. Increased release of elastase and of oxygen radicals after stimulation with opsonized bacteria.

In 12 patients with juvenile periodontitis (JP), stimulation of peripheral polymorphonuclear neutrophils (PMN) by opsonized bacteria showed increased release of free oxygen radicals and of elastase activity in relation to that of pair-matched healthy controls. The elastase increase was not associated with higher intracellular content of elastase, since the measurement of homogenized PMN cells did not differ between the patient group and the control group; nor did the spontaneous elastase activity of nonstimulated cells differ. Release of elastase by contaminating lymphocytes and platelets could be excluded, since no elastase activity from these cells or interference by these cells could be demonstrated in the assay system used. When formyl-methionyl-leucyl-phenylalanine was used as a stimulant, the chemiluminescence in the patient PMN cells did not differ with statistical significance from their pair-matched controls. The increased release of free oxygen radicals and of elastase seems to be a characteristic of the PMN cells in juvenile periodontitis, indicating hyperactive cells with possible pathogenic effects.

Adolescent↗

PubMed1988

Peripheral PMN cell activity in relation to treatment of juvenile periodontitis.

Ten patients with untreated juvenile periodontitis (JP) showing an increased generation rate of free oxygen radicals from their peripheral polymorphonuclear neutrophils (PMN) as measured by luminol enhanced chemiluminescence (CL) were rechecked after treatment. The PMN cells from eight patients still reacted with higher CL after stimulation with Staph. aureus opsonized with autologous serum when related to pair-matched controls. The increased CL was not caused by adsorption of serum factors from untreated JP patients when assayed with healthy PMN cells. The increased CL found in peripheral PMN cells from patients with JP thus depends very little on the disease activity.

Adolescent↗

PubMed1988

Plasma elevations of histamine and a prostaglandin metabolite in acute asthma.

Recent studies of laboratory-provoked asthma have suggested that asthma is an inflammatory disease of lower airways. The purpose of this study was to measure the systemic elaboration of 2 bronchoconstrictive inflammatory mediators during naturally acquired acute asthma utilizing a prospective, serial-sampling protocol. Plasma levels of 13,14-dihydro-15-keto-PGF2 alpha and histamine were measured by radioimmunoassay and radioenzymatic assay, respectively, in 23 children with acute asthma. Mean PG metabolite and histamine values (pg/ml) before (167 +/- 72, 1,029 +/- 378) and 10 to 90 min after (377 +/- 145, 1,000 +/- 489) initial therapy were significantly higher than those of the same children after resolution of asthma (2.9 +/- 0.2, 260 +/- 42) and those of normal children (4.3 +/- 0.9, 240 +/- 14). Peak PG metabolite levels were significantly higher in children who presented with PEFR values (% predicted) less than 40% (1,234 +/- 432) compared with those who presented with greater than 40% (404 +/- 296), and in children with post-therapy improvement in PEFR of less than 20% (1,281 +/- 470) compared with those with greater than 20% (365 +/- 226). Histamine levels were significantly higher in children with post-therapy improvement in PEFR of less than 20% (2,560 +/- 1,600) compared with those with greater than 20% (475 +/- 100), and in hospitalized (3,915 +/- 1,910) compared with nonhospitalized (408 +/- 130) children. Significant differences were not observed on the basis of corticosteroid dependence, allergic disposition, or type of initial therapy. These data suggest a role for histamine and PGF2 alpha in the pathogenesis of airway inflammation in acute asthma.

Acute Disease↗

PubMed1986

Influence of plasma components on luminol-enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis.

The generation rate of free oxygen radicals as measured by maximal light intensity of luminol-enhanced chemiluminescence from peripheral blood granulocytes (PMN) stimulated with differently opsonized Staphylococcus aureus was studied in 13 patients with juvenile periodontitis (JP) and pair-matched, healthy controls. Plasma proteins related to inflammation were also assayed. When stimulated with bacteria opsonized with autologous serum, the PMN from the JP patients showed a more intensive chemiluminescence than did their pair-matched controls (p less than or equal to 0.0005). The difference was consistent but slightly reduced when using heat-treated serum (p less than or equal to 0.006) or heterologous gammaglobulin (p less than or equal to 0.19) for opsonization. When testing freeze preserved sera from 11 of the compared pairs, the sera from JP patients induced a slightly higher chemiluminescence in PMN from a healthy donor (p less than or equal to 0.031). Protein analysis of the patient sera revealed a slightly higher concentration of complement 4 (p less than or equal to 0.032) and IgM (p less than or equal to 0.030) when compared with their respective pair-matched healthy controls. The influence of other blood components contaminating our assay system was checked on healthy PMN cells. Lymphocytes, platelets, relevant amounts of ADP and serum had no effect on the chemiluminescence. In conclusion, the increased chemiluminescence of peripheral blood granulocytes from patients with juvenile periodontitis seems to be related mainly to the cells. The association with free oxygen radicals and their tissue-damaging potency might be a contributing factor in the pathogenesis of periodontal disease.

Adolescent↗

PubMed1984

Increased luminol enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis.

The granulocyte function in patients with juvenile periodontitis (JP) (eight men and six women, aged 13-33 yr) and sex and age matched controls was determined by the luminol enhanced chemiluminescence (CL) induced by opsonized and unopsonized bacteria or latex beads. Maximal CL induced by latex beads with or without autologus serum did not differ between the two groups. However, bacteria with or without autologous serum seemed to induce a higher maximal CL in the JP group. The difference was statistically significant for the results obtained with opsonized bacteria. Serum opsonized bacteria induced a much higher maximal CL than the unopsonized. The opsonin dependent reaction indicates a close association with the process of phagocytosis. The increased CL of granulocytes in JP could indicate the formation of extracellularly free oxygen radicals with the potential to damage tissue. These observations support a possible involvement of the granulocytes in the pathogenesis of JP.

Adolescent↗

PubMed1981

Ratio of alpha-1-antitrypsin to transferrin in gingival fluid and in blood from patients with periodontal disease.

In 30 men and 29 women the mean gingival exudate ratio alpha-1-antitrypsin (A1AT)/transferrin (Tr) was higher than the mean blood ratio for A1AT/Tr, when determined by double electroimmunoassay. It suggests that A1AT penetrates the tissue more easily than transferrin probably due to its smaller molecular size or that migration into the extracellular space occurs due to consumption by proteolytic enzymes. The gingival exudate ratio and the blood ratio for A1AT/Tr were not related to periodontal disease when considered separately. However, the difference between the exudate ratio and the corresponding blood ratio was significantly less (P = 0.02) for individuals with periodontal disease. This suggests that the ratio difference is of importance in the destruction of tooth-supporting collagen fibers.

Adult↗

PubMed1980

The relationship between marginal bone loss and serum zinc levels.

Serum was analyzed for zinc in 51 patients of varying age and with varying degrees of alveolar bone loss as recorded on roentgenograms. There was a reversed correlation between marginal alveolar bone loss and serum zinc levels. The observations are discussed in relation to the physiological functions of zinc.

Adult↗