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Biomedical subjects

B Bénard

Publications and source records attributed to B Bénard.

14 recordsLinked to original sources
PubMed1983

Nuclear androgen receptors of human prostatic tissue--a quantitative histological study.

The objective of this study was to determine the nuclear dihydrotestosterone (DHT) nuclear receptors content in tissues of normal prostate, benign prostatic hypertrophy (BPH), and prostatic cancer. We also attempted to correlate this with the histological pattern of these tissues. Specimens were obtained from five normal subjects, 24 BPH, and 19 prostatic cancer patients. Nuclear receptors content was determined. All histological sections were reviewed by three independent pathologists. The criteria for the quantitative analysis were the degree of cellularity and the stromal content. The mean receptor content was not significantly different between neoplastic tissue (52.6 fmol/mg protein) and hyperplastic tissue (38.9 fmol/mg protein). The number of binding sites in normal prostatic tissue was 8.5 fmol/mg protein. The correlation between the degree of cellularity and DHT receptors showed no significant difference for both BPH, as well as prostatic cancer. Similar results were obtained when stromal content was compared.

Adenocarcinoma↗
PubMed1980

Dihydrotestosterone receptors in the human prostate. I. Nuclear concentration in normal, benign, and malignant tissues.

A technique was standardized to measure nuclear dihydrotestosterone receptors in human prostate. Normal, hyperplastic (BPH), and cancer tissues were homogenized and the homogenate centrifuged at 600 g X 10 min; the pellet was purified on sucrose density gradient. Purified nuclei were extracted with 0.4 M KC1. The nuclear extract was then incubated in the presence of [3H]dihydrostestosterone (DHT) at 4 degrees C for 20 hr. Competition studies with different steroids indicated a high specificity of the receptor towards DHT and no affinity towards progesterone and 17 beta-estradiol. Isolation and purification of [3H]DHT after its incubation in the presence of the KC1 nuclear extract indicated that no significant degradation of the hormone occurred during incubation. The number of binding sites measured was 8.5 +/- 0.77 fmol/mg protein (mean +/- SEM) for normal, 38.6 +/- 5.9 for BPH, and 79.9 +/- 21.9 for cancer. The possible clinical application of these results will require a long-term follow-up of the hormonal responsiveness of the prostatic-cancer patients investigated.

Binding, Competitive↗
PubMed1978

[Growth hormone specific binding sites : characteristics of lactogenic receptors induced by estrogens (author's transl)].

In vitro binding of growth hormone was characterized in rat liver. Microsomal preparations were found more active than membranes purified with an aqueous two-phase polymer system. Binding conducted at 4 degrees C was found optimal after 72 hours of incubation in 5 mM Tris-Maleate buffer pH 6.4 with 25 mM CaCl2. Injecting estrone (25 microgram/100 g B.W.) for one week induced the formation of lactogenic receptors, and increased the specific binding of hGH from 2.8 +/- 1.9 to 22.3 +/- 7.1%. Prolactin and hGH, but not rGH or other pituitary hormones, could displace radioactive hGH. With incubations conducted at 37 degrees C, equilibrium was reached more rapidly but at the cost of a more extensive degradation. The presence of membrane receptors in the medium partly protected the hormone against aggregation and degradation. Scatchard plots were obtained from experiments conducted under optimal conditions and analyzed on computer using a program based on an iterative method. Data indicated that lactogenic receptors possessed a single specific binding site for hGH with a constant (Ka) of 2.18 +/- 0.22 x 10(9) M-1 and a binding capacity of 304 +/- 91 fm/mg proteins.

Animals↗
PubMedDate not supplied

Pigmented adrenal hyperplasia with myelolipomatous changes and bilateral testicular enlargement in an untreated man with 21-hydroxylase deficiency.

OBJECTIVE: To report the occurrence of a giant left adrenal tumor and bilateral testicular masses (adenomatous hyperplasia of Leydig cells) in a young man with congenital adrenal hyperplasia due to 21-hydroxylase deficiency. METHODS: The clinical, radiologic, endocrinologic, and pathologic features of this case are correlated with the findings in the literature. RESULTS: The interesting elements in this case are the rare pathologic features of the left adrenal lesion (pigmented adrenal hyperplasia with myelolipomatous changes) and the association with the infrequent testicular adrenal rest tumors. The absence of enlargement of the right adrenal gland was unexplained. CONCLUSION: The presence of these two rare complications seemed to be associated with poor adherence to medical treatment recommendations for congenital adrenal hyperplasia.

Adenoma↗