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Biomedical subjects

B Böthig

Publications and source records attributed to B Böthig.

11 recordsLinked to original sources
PubMed1992

Qualification of long-stored samples of serum banks for seroepidemiological studies.

Serum samples were tested for antibodies against polio virus and measles virus from two serum banks after having been stored under different conditions for several years. The results are compared with those ones obtained immediately after sampling. Despite of small increases of antibody-negative samples and of small decreases of the titres the suitability of the samples stored could be shown. Special attention should be given to the establishment, e.g. in the framework of Expanded Programme on Immunization of the World Health Organization and in order to study new pathogens unknown to date, and the technical preconditions for that should be planed.

Antibodies, Viral↗
PubMed1992

Production of monoclonal antibodies to rotavirus suitable for detection of rotavirus in stool samples by one-step EIA.

Two stable hybridoma cell clones producing high amounts of monoclonal antibodies (MAb) to rotavirus have been selected. The MAbs were shown to recognize different epitopes of the major inner capsid rotavirus protein VP 6. The two types of MAb in question cross-reacted with human and animal group A rotaviruses. Due to their high affinity and good binding capacity to microtiter plates a simultaneous EIA was developed for detection of rotavirus in stool samples. The sensitivity and specificity of MAb one-step EIA was compared with an approved polyclonal sandwich EIA by testing 1309 stool samples.

Animals↗
PubMed1990

Immunogenicity of oral poliomyelitis vaccine (OPV) against variants of wild poliovirus type 3.

Serological investigations of three groups of children from the German Democratic Republic (GDR) and from Czechoslovakia who had different immunization histories against poliomyelitis indicated that the immunity induced by oral poliovaccine (OPV) is effective against both the wild poliovirus Saukett strain and a new wild variant of poliovirus type 3 that was isolated during an outbreak of poliomyelitis in Finland in 1984. There is therefore no obvious risk that individuals in the GDR or Czechoslovakia, most of whom have been immunized with OPV, are threatened by new wild poliovirus variants. These findings are of importance, especially in connection with WHO's initiative for the global eradication of poliomyelitis by the year 2000.

Antibodies, Viral↗
PubMed1990

[The expanded WHO vaccination program and the vaccination strategy in East Germany].

Evaluating the current stage and future trends of WHO's Expanded Programme on Immunization (EPI), conclusions for national immunization strategies have been considered. The global eradication of poliomyelitis is an important target. In 1988, an Immunization Advisory Group of the Ministry of Health has been founded. Recommendations regarding the update of immunization schedule have been elaborated. Changes are foreseen for BCG (deletion of revaccination), rubella (implementation of immunization for all girls aged 11/12 years), diphtheria (diphtheria/tetanus booster for adults), and measles (2-shot-strategy for young children and schoolchildren.

Child↗
PubMed1989

Atypical human rotaviruses in the G.D.R.

Following the aetiologic agent of diarrhoea in children aged one to three years we found in the stools a large number of rotaviruses by electron microscopy (EM), although ELISA was negative; the sensitivity of ELISA had been confirmed in previous investigations. In 141 children with diarrhoea 10 conventional (group A) and 21 antigenically distinct rotaviruses were detected. The presence of atypical rotaviruses could be confirmed by electrophoretic analysis of their RNA migration profiles. The electropherotype pattern corresponded to the group C rotaviruses reported by others.

Antigens, Viral↗