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Biomedical subjects

B Banerjee

Publications and source records attributed to B Banerjee.

At least 19 recordsLinked to original sources
PubMed1997

Identification of 45 kD antigen in immune complexes of patients of allergic bronchopulmonary aspergillosis.

A glycoprotein antigen with an apparent mw of 45 kD was observed to be predominant in the circulating immune complexes isolated from patients of allergic bronchopulmonary aspergillosis as well as in the immune complexes prepared in vitro. Further characterisation of 45 kD antigen with trypsin showed four immunologically active peptides with mw 43, 36, 33 and 16 kD. Carbohydrate characterization using various lectins (Maackia amurensis agglutinin, Sambucus nigra agglutinin, Peanut agglutinin, Galanthus nivalis agglutinin and Datura stramonium agglutinin) showed presence of both N-linked and O-linked sugar moieties such as mannose, glucose, galactose and N-acetyl glucosamine. Predominant presence of 45 kD antigen in immune complexes, recognition of 45 kD by monoclonal antibodies raised against glycoprotein rich fraction of A. fumigatus and presence of elastinolytic protease activity indicate that 45 kD antigen is probably a potent virulence factor and may be contributing to the pathogenesis of ABPA by its biological as well as immunological activities.

Adult↗
PubMed1996

Thermodynamics of the interaction of d-tubocurarine with nicotinic receptors of mammalian skeletal muscle in vitro.

Thermodynamic study is important for defining drug receptor interactions, and denervated rat hemidiaphragm is a unique preparation for such a study on nicotinic receptors. As a continuation of our earlier study with acetylthiocholine on the same preparation, we now report on the characteristics of temperature-dependent binding of d-tubocurarine, a reversible antagonist. The O. Arunlakshana and H.O. Schild (1959, Br. J. Pharmacol. 14, 48) equation, as improved by D.R. Woud and R.B. Parker (1971, J. Pharmacol. Exp. Ther. 177, 13), was used to calculate the dissociation constant of d-tubocurarine at various temperatures (10-37 degrees C) from the parallel shift of the acetylcholine dose-response curve to the right by effective doses of d-tubocurarine. It was observed that the values of the dissociation constant increased with a decrease in temperature. Both the enthalpy (delta H degree) and entropy (delta S degree) changes as evaluated from the van't Hoff plot (In Kd vs. 1/T) were found to be positive and their relative value (delta H degree - T delta S degree) produced a negative free energy change which characterises the binding of d-tubocurarine as an entropy-controlled process. This finding is in agreement with the neurotoxin binding reported earlier. The present finding and earlier observations with acetylthiocholine reveal that agonist and antagonist binding to the nicotinic receptor may differ depending on the experimental conditions.

Animals↗
PubMed1995

Thermodynamic studies with acetylthiocholine on nicotinic receptors of mammalian skeletal muscle in vitro.

The temperature dependency of binding of acetylthiocholine, a specific nicotinic agonist, to the nicotinic receptor of mammalian skeletal muscle was studied using isotonic contractions of the rat denervated diaphragm preparation in vitro. The dissociation constants at different temperatures (22-39 degrees) were determined by the Furchgott method using alpha-bungarotoxin as an irreversible antagonist. Both free energy of association (delta G zero = -22.93 kJ/mol at 37 degrees) and enthalpy of binding (delta H zero = -58.35 kJ/mol) calculated from Kd (dissociation constant) and slope of lnKd versus 1/T (van't Hoff plot) respectively were found to be negative. The negative entropy value (delta S zero = -0.113 kJ/mol/deg) obtained from the intercept of this van't Hoff plot differs from the large positive value obtained earlier employing radioligand binding studies of the nicotinic receptor of Electrophorus electricus.

Acetylthiocholine↗
PubMed1995

Nonsurgical management of primary cholangiocarcinoma. Retrospective analysis of 40 cases.

Forty patients with cholangiocarcinoma (23 men, 17 women) underwent nonsurgical palliative biliary drainage over a period of 12 years. All were surgically unfit or had unresectable disease. All were jaundiced at presentation with a mean serum bilirubin of 11.5 +/- 1.9 mg/dl. Thirty patients (75%) had hilar obstruction. Twenty-eight were drained percutaneously, three endoscopically and nine by a combined endoscopic and percutaneous procedure. Technical success was 97.5%. Final mean bilirubin was 1.5 +/- 0.4 mg/dl. Minor complications occurred in 10 (25%) patients, and major complications in four (10%). Procedure-related mortality was 2.5% with a 30-day mortality of 7.5%. Mean survival was 8.2 +/- 0.5 months. Stent changes were required in eight patients. In patients with inoperable or unresectable cholangiocarcinoma, percutaneous or endoscopic biliary drainage offers effective palliation.

Aged↗
PubMed1995

A crowded ureter.

Explore the source record for details and available documents.

Foreign-Body Migration↗
PubMed1994

The epidemiology of fractured femurs and the effect of these factors on outcome.

We have analysed 250 cases of fractured shaft of the femur with the objective of highlighting any preventative measures and anticipating morbidity associated with cause and type of injury. The younger patients typically injure themselves as pedestrians, motorcyclists and bicyclists in Summer. The older patients tend to have falls in late Summer and Winter. The outcome varies depending on the cause, type and age of the injured person. The greatest morbidity was found in high-velocity accidents involving motorcyclists, pedestrians and car accidents. Knee flexion is a particular problem with these injuries and we are currently using continuous passive motion in conjunction with a continuous opiate infusion where this is likely to be a problem. The results also suggest that the mechanism of injury and its possible prevention, particularly in motorcycle design, could be as important as the type of treatment. Elderly females following falls also form a distinct group, suggesting that osteoporosis is a major factor with the best outcome associated with treatment by internal fixation. We feel that age alone should not be a reason for exclusion from intramedullary fixation and this merits further study.

Accidental Falls↗
PubMed1994

Injuries associated with a fractured shaft of the femur.

A knowledge of injury patterns, both from the mechanism of injury and from their associations, allows specific injuries to be suspected and actively excluded. We have reviewed fractures of the femoral shaft to highlight the patterns of their associated high morbidity and mortality. Our survey shows that accidents involving motorcyclists, pedestrians and motor cars have the highest incidence of associated injury. Common associations include chest, head, pelvis and ipsilateral leg injuries in pedestrians, and pelvic and ipsilateral leg injuries in motorcyclists. Rarer associations with femoral shaft fractures include ipsilateral tibia and talus/navicular fractures in motorcyclists, knee injuries and contralateral acetabular fractures in pedestrians and contralateral necks of femur in motor car passengers.

Accidental Falls↗
PubMed1993

Serological findings in patients with allergic bronchopulmonary aspergillosis during remission.

The concentrations of total IgG, complement C3 and circulating immune complexes (CIC) were studied in 15 patients with allergic bronchopulmonary aspergillosis (ABPA) during remission and compared with those of 10 matched controls. The concentrations of complement and circulating immune complexes were similar to those of the controls but 33% of patients with ABPA in remission still had circulating immune complexes, mainly of IgG. Even so, the concentrations of IgG were significantly higher in patients during remission compared to controls but were lower than those observed during the acute stage of ABPA.

Acute Disease↗
PubMed1993

Extrahepatic biliary tract obstruction. Modern methods of management.

Extrahepatic biliary tract obstruction can often be diagnosed by means of thorough history taking, physical examination, and basic laboratory data. The cause of obstruction can be best defined and is often best treated by biliary endoscopy and/or percutaneous radiologic techniques, which appear to be safer than surgical decompression.

Cholangiography↗
PubMed1992

An unusual complication of paracentesis.

Paracentesis is an important and commonly performed procedure in patients with ascites. It is a safe procedure when carried out in the midline below the umbilicus, with a complication rate of less than 1%. We report an instance in which a large midline varix was entered during paracentesis. The utility of different imaging techniques in detecting such anomalies in the portal hypertensive patient with portal hypertension and ascites is discussed. The approach and management of this complication are outlined.

Abdominal Muscles↗
PubMed1991

Sonographic detection of foreign bodies of the extremities.

A prospective study to assess the accuracy of ultrasound in the detection and localization of foreign bodies of the extremities was undertaken. Forty-five patients with clinical suspicion of foreign bodies were included in the study. Among 20 patients with radio-opaque foreign bodies, ultrasonic detection of foreign bodies was achieved in 19 patients. In 25 patients, with no radiographic evidence of foreign body, ultrasonic detection of foreign bodies was made in seven patients and among these foreign bodies were confirmed and removed at surgery in five patients. Accurate pre-operative ultrasonic localization of foreign bodies was carried out in three patients.

Evaluation Studies as Topic↗
PubMed1991

Ultrasound diagnosis of horseshoe kidney.

Ultrasound diagnosis of horseshoe kidneys depends on the demonstration of an isthmus or band of renal tissue across the midline of abdomen connecting the lower poles of the kidneys. In a number of cases this band of renal tissue may evade ultrasonic detection. In a series of 11 patients with horseshoe kidneys, sonographic detection of renal isthmus was achieved in seven patients, but in all 11 cases there was demonstrable alteration of renal outlines. Instead of normal reniform shape, the kidneys appeared "inverted pyriform" or "inverted triangular". Detection of such an altered renal outline should raise suspicion of horseshoe kidney.

Child↗