Biomedical subjects

B Bartolomé Zavala

Publications and source records attributed to B Bartolomé Zavala.

3 recordsLinked to original sources

PubMed2006

Mantis shrimp allergy.

We report the case of a 25-year-old female who experienced two immediate episodes of labial, lingual and larynx angiodema after eating fried mantis shrimp (Squilla empusa), a crustacean belonging to the Squillidae family, and a third episode after eating shellfish pudding. Prick by prick tests to mantis shrimp and to other crustacean were positive. Serum specific IgE showed higher values than 0.35 kU/L to extracts from mantis shrimp shell, prawn, large prawn and crab. Serum specific IgE against large prawn tropomyosin were below 0.35 kU/L. Sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) immunoblotting results with raw mantis shrimp shell revealed IgE-binding bands of 46 kDa and 25 kDa, in non reducing and reducing conditions, respectively. Cross-reactivity studies using the enzyme allergo sorbent test (EAST)-inhibition and Immunoblotting-inhibition methods showed the existence of significant cross-reactivity between the IgE-binding proteins present in mantis shrimp and those from prawn, large prawn and small crab.

Adult↗

PubMedDate not supplied

Barnacle hypersensitivity.

BACKGROUND: the aim of the present study is to investigate the responsible mechanism of different adverse reactions suffered by five patients, aged between six and thirty years-old, after consumption of barnacle. The symptoms were angioedema, dyspnea, generalized urticaria, conjunctivitis and one of them suffered from anaphylactic reaction. Four patients had personal atopic history. METHODS: the allergic study included prick by prick test with raw and boiled barnacle and prick-test with a standardized battery of shellfish and neumoallergens, specific-IgE determination to barnacle, crustacean and house-dust-mite and SDS-PAGE immunoblotting to barnacle. Even though an oral challenge was proposed to three of the patients, they were reluctant to do the test and eventually the challenges were not carried out. RESULTS: prick to prick tests were positive to barnacle for all of them. Specific-IgE was found in four patients. The western blotting results showed an IgE-binding band whose apparent molecular mass ranged between 58 and 68 kDa. CONCLUSIONS: barnacle could induce IgE-mediated adverse reaction. Our study has demonstrated the presence of an IgE-binding protein in barnacle extracts ranged between 58 and 68 kDa of molecular mass. It has not been previously described a crustacean allergen with the same molecular mass, so it could be a specific allergen from barnacle. We believe that further study will confirm this is the case.

Adult↗

PubMedDate not supplied

Non-occupational allergy caused by the pine processionary caterpillar (Thaumetopoea pityocampa).

UNLABELLED: Contact with the pine processionary caterpillar induces dermatitis, usually located in exposed areas, and, less frequently, ocular lesions through a toxic-irritative mechanism. Recently, the existence of an immediate hypersensitivity mechanism has been demonstrated, mainly in occupationally exposed patients. OBJECTIVE: To present four patients who experienced allergic reactions (urticaria-angioedema and rhinitis-asthma) after non-occupational exposure to pine processionary caterpillar. PATIENTS AND METHODS: The four patients underwent allergy testing through skin prick tests (SPT), specific IgE detection and SDS-PAGE immunoblotting. One patient also underwent a specific bronchial challenge test with the pine processionary antigen. RESULTS: In all patients, both SPT with the caterpillar extract and specific IgE were positive. Western blotting showed several IgE-binding bands with molecular mass values ranging from 18 to 107 kDa. A shift in the electrophoretic mobility of some of the relevant allergens occurred under the presence of a reductive agent (beta -mercaptoethanol). The specific bronchial challenge test with pine processionary antigen performed in one of the patients also produced positive results. CONCLUSIONS: The results of this study show an immunologic IgE-mediated immediate hypersensitivity mechanism in these reactions. The processionary caterpillar's airborne urticating hairs or spicules should be considered, at least in some locations, not only as contact and occupational allergens, but also as seasonal aeroallergens.

Adult↗