Biomedical subjects
Publications and source records attributed to B Basak.
Three new sulfur reagents for specific identification of amino acids on thin-layer chromatography plates have been introduced. These three sulfur containing reagents are capable of developing various distinguishable colors with many of them. A probable mechanism for such color formation is proposed.
To study the antifertility effect of an extract (alcoholic) of the leaf-stalk of Piper betle Linn., one set of experiments with two different doses in Swiss male albino mice were evaluated. Initially, 500 mg of the leaf-stalk extractive for 30 days and then 1000 mg for next 30 days/animal/day/kg body weight were administered orally. The extract reduced fertility to 0% within 60 days. Suppression of cauda epididymal sperm count and motility (p <0.05) was observed. Biochemical parameters did not show any marked alterations in testosterone content in serum nor 17beta-hydroxysteroid dehydrogenase (17beta-HSD) activity in testes although fructose content in seminal vesicles was reduced as are the weights of reproductive organs. The cholesterol content in testes increased, although not appreciably. After cessation of drug (plant extract) treatment, the altered parameters recovered. Results suggest that the contraceptive effect of the extract of leaf-stalk of Piper betle Linn. is mainly on the maturation process of spermatozoa in epididymides without influencing hystemic hormonal profiles. Withdrawal of the extract restored all altered parameters including organ weights and fertility after 60 days.
The structure and stereochemistry of a new terpenoid ester, nardostachysin (1), isolated from the rhizomes of Nardostachys jatamansi, were established as the 7',8'-dihydroxy-4'-methylene hexahydrocyclopenta[c]pyran-1'-one-8'-methyl ester of 7, 9-guaiadien-14-oic acid, by spectral and chemical studies.
Description of a new species of sandfly Phlebotomus sundarai sp. nov. collected from Kala-azar endemic blocks-Gosaba and Baruipur, of district South 24-Parganas, West Bengal is given.
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Anopheline fauna of cattlesheds (CS) in Ajodhya hills, District Purulia, consisted of 12 species during summer, monsoon and 10 species during winter. Of the six vector species encountered in CS, Anopheles culicifacies, An. annularis, An. subpictus, An. maculatus and An. fluviatilis were found in all the three seasons. An. philippinensis was encountered in monsoon only and constituted 0.61% of the total anopheline catch made during the season. An. culicifacies was the most predominant species during summer (MHD 30.38) and monsoon (MHD 11) but ranked third (MHD 9.64) to An. annularis (MHD 16.28) during winter; An. subpictus being second (MHD 10.28) in order of predominance. An. annularis was the second most prevalent vector species in summer (MHD 21.38) and monsoon (MHD 9.92). The population density of the remaining three vector species was extremely low in the two seasons. In human dwelling (HD), of the five anopheline species encountered (An. subpictus, An. annularis, An. culicifacies, An. vagus and An. splendidus), the former three were found in all the three seasons. MHD of An. annularis in HD in summer, monsoon and winter was 3.7, 1.1 and 1.9 respectively, while MHD of An. culicifacies in the corresponding seasons was 5.01, 2.09 and 1.31 respectively.
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Studies have been carried out on the protein solubility profile of Kulthi (Macrotylona uniflorus, Lam.) seed in aqueous solution over various pHs and at different concentrations of NaCl, Na2SO3, CaCl2, and MgCl2 at pH 8.0. Amino acid analysis of isolated protein identified 17 amino acids, 9 of which are essential. Gel-permeation chromatography on Sephadex G-200 revealed the presence of seven components in the protein fraction. Their molecular weights were determined by two comparable standard methods. Extractable Kulthi seed proteins in salt solutions were separated electrophoretically into eight fractions whose molecular weights were found to be 186,200, 131,800, 108,400, 91,200, 53,700, 44,700, 38,000, and 27,500.
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The effect of protein, isolated from Jute (Corchorus olitorius) seed was studied upon albino rats with respect to some of their serum, liver and intestinal enzymes and liver lipids. An increase in the body weight (including the weight of the liver) was noted in test animals after feeding with a Jute seed protein enriched diet. It was also observed that AST, ALT and total lipid of liver increased significantly whereas AST and ALT of serum were decreased. An increase in the concentration of lipids in the liver was found and this may be due to excess of the seed protein in the diet. An overall observation reveals that there is slight fatty infiltration in the liver of test animals.
The influence of protein, isolated from teak (Tectona grandis) seed upon albino rats with respect to some of their serum, liver and intestinal enzymes and liver lipids has been studied. The protein in question contains aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, lysine, phenylalanine, histidine and arginine as determined by amino acid analyser. After feeding experiment an increase in body weight including the liver weight was noted in the test animals due to excess protein in the diet. A marked increase was observed in G.O.T., G.P.T. and total lipid of liver, whereas G.O.T. and G.P.T. of serum were decreased. The observed increased concentration of lipid in liver may be due to excess addition of protein in diet. The overall observation is an indication of probable fatty infiltration in liver of test animals.
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Extracts from different parts of plants (1) including seeds are reported to act upon pathogenic microorganisms. A few active principles were isolated. The present preliminary report shows significant role of the seeds of Dolichos biflorus (Linn), locally known as Kulthi, against some test organisms using different fractions of defatted seed extracts with methanol.