Biomedical subjects

B Belić

Publications and source records attributed to B Belić.

6 recordsLinked to original sources

PubMed1996

[Transfusion aspects of viral hepatitis C].

The investigation comprised 83 patients with acute non-A, non-B hepatitis who were treated at the Clinic of Infectious and Dermatovenerology Diseases in Novi Sad in the period 1986-1991. They were receiving blood transfusions and/or blood products from blood donors who had not been previously tested for presence of hepatitis C virus (HCV). In order to establish diagnosis anti-HCV ELISA screening tests from various producers were used. Out of 83 blood screening assays 17 patients (20.48%) were found to be anti-HCV seropositive. Out of these 17 seropositive patients 3 patients were intravenous drug-addicts (IVDA), while 14 patients (16.68%) previously received blood transfusions, whereas out of these 7 patients underwent open heart surgery. Only 2 blood donors, out of 48 whose blood was used for therapeutical purposes, were found to have anti-HCV antibodies after blood screening assays. The blood with detected anti-HCV antibodies caused acute post transfusion hepatitis C in 6 blood recipients. The obtained results show that comprehensive and thorough blood screening assays should be performed in all blood donors in order to prevent incidence of post transfusion C hepatitis. The Blood Transfusion Center in Novi Sad introduced compulsory viral screening assays for all blood donors on November 15, 1994, and since then 15019 blood donors have been tested. Only 38 (0.25%) were found to be anti-HCV seropositive, and were excluded from further blood donation and are under constant control. Now it is impossible to transmit HCV to patients who receive blood transfusion and/or blood products, and at the same time anti-HCV seropositive donors are prevented to develop chronic hepatitis and adverse complications due to HCV presence.

Adolescent↗

PubMedDate not supplied

[Complications in the use of blood transfusions--alloimmunization in polytransfused patients].

INTRODUCTION: The objective of this paper was to examine the frequency of red blood cell (RBC) alloantibodies in polytransfused hematologic patients. MATERIAL AND METHODS: Blood samples of 2669 polytransfused hematologic patients were examined on clinical significant alloantibodies using antibody screening and identification according to Standards of AABB Technical Manual (1). Available medical charts were reviewed for sex, age and medical history and total number of given transfusions. RESULTS: During a three year period blood samples of 2669 polytransfused hematologic patients were examined for RBC alloantibodies. Alloantibodies were detected in 48 cases with the incidence of 1.79%. 36 patients (1.35%) developed single antibody while in 12 patients (0.45%) multiple antibodies were detected. Antibodies were registered more frequently in females than in males (37:17). In patients with single antibody next specificity was detected: anti-D (38.89%), anti-K (22.22%), antibodies to antigens MNSs system (22.22%), while anti-Le, anti-Fy and anti-P specificity was detected in 13.89%. Patients with multiple antibodies had specificity to Rhesus, Kell, Duffy, MNSs, Lewis and P blood group systems. All patients received more than 10 RBC transfusions. CONCLUSION: The incidence of alloimmunization was 1.79%. Sensibilization occurred more frequently in females than in males. Usually, the discovered alloantibodies were clinically significant and made problems in pretransfusion testings and required special efforts in blood selection for transfusion. For patients with the risk of frequent transfusions we suggest to include blood transfusion charts with complete phenotyping against antigens in Rhesus, Kell, Kidd and MNSs blood group systems and the data of all received transfusions.

Erythrocytes↗

PubMedDate not supplied

[Implementation of a quality control system in transfusion practice].

Quality assurance and improving systems, standardization of all transfusion procedures as well as quality control and standardization of prepared blood products are necessary elements for work of transfusion centers in the future. Requirements for blood and/or blood products for therapy are: safety, adequate concentration of all active components needed, and treatment efficacy. To satisfy these requirements, we decided to adjust the work process of transfusion service to requirements of ISO 9001 standard series. To achieve this aim, quality control system documentation is created. Creation of quality control system documentation is one of the most difficult and most comprehensive parts the quality control system implementation process. The quality policy is adopted, whereas the basic procedures for blood products quality control, which are the subject of this paper, are created.

Blood Transfusion↗

PubMedDate not supplied

[The HLA antigen system in married couples with recurrent spontaneous abortions].

INTRODUCTION: Investigations of factors responsible for the disturbance of fetomaternal tolerance during pathological pregnancies and causes of recurrent spontaneous abortions are pointing that HLA antigens play a key role in their development. Rejection of semiallogeneic allograft in this pregnancy disorder is a consequence of interaction between genetic and immunologic phenomena. MATERIAL AND METHODS: This article reports results of an investigation of human lymphocyte antigens in 130 couples with recurrent spontaneous abortions and in control group of 57 healthy couples. In both investigated groups the first class of human lymphocyte antigens was detected using microlymphocytotoxicity test by Terasaki. The phenotypic frequencies in the group with recurrent spontaneous abortions were compared with the corresponding results in the control group. We investigated the relative risk for development of this disorder and statistically significant difference of human lympocyte antigen frequencies between both investigated groups. RESULTS: The results of investigation showed that in the group of couples with recurrent spontaneous abortions the relative risk is hihger than 1 for the following human lymphocyte antigens: A2 = (1.53), A9 = (1.149), A10 = (1.07), A29 = (1.70), B18 = (2.24), B40 = (3.31). A significant difference in human lymphocyte antigen frequencies was established for HLA A2, HLA B18 and HLA B40, in comparison with the results of control group. Couples with recurrent spontaneous abortions had a statistically significantly higher rate of identical human lymphocyte antigens among spouses in comparison with the control group, at the level of significance p < 0.05 = 6.32. CONCLUSION: The results of this investigation showed that human lymphocyte antigens play an etiopathogenic role in development of recurrent spontaneous abortions and that some of these antigens are increasing susceptibility to pregnancy disorders.

Abortion, Habitual↗

PubMedDate not supplied

[Association between HLA antigens and leukemia in the population of Vojvodina].

INTRODUCTION: One of the most fascinating areas of research within the field of histocompatibility at present time concerns an observation that a major human histocompatibility system, HLA, is deeply involved in development of a great number of diseases. MATERIALS AND METHODS: HLA class I antigens were investigated in 225 cases with various kinds of leukemia: 112 patients with acute myeloblastic leukemia (AML), 31 with acute lymphoblastic leukemia (ALL), 44 with chronic myelocytic leukemia (CML) and 38 with chronic lymphocytic leukemia (CLL). Applying method of microlymphocytotoxicity, 13, 19 and 8 antisera were used for A, B and C loci respectively. Control group comprised 300 unrelated persons, whose phenotypic frequencies were used to calculate the relative risk (RR), while for values of RR greater than 1, we calculated etiologic fraction (EF), and for negative RR values we calculated preventive fraction (PF). RESULTS: Results of investigation showed that RR in AML was: for A2 = 1.144, for A3 = 1.038, for A29 = 1.814, for A34 = 2.69, for B7 = 1.06, for B14 = 1.74, for B17 = 1.65 and for B21 = 2.49 and B35 = 1.77 with value of chi 2 test of 4.62 and 4.63; that RR in ALL was: for A1 = 1.61, for A2 = 1.1, for A10 = 1.23, for A11 = 1.57, for A30 = 1.4, for A32 = 2.2, for B7 = 2.81 with value of chi 2 test 4.39; that RR in CML was: for A2 = 1.21, for A32 = 1.89, for B7 = 1.52, for B12 = 1.2 and for B15 = 3.28 with value of chi 2 test of 5.89; and that RR in CLL was: for A1 = 1.35 with value of chi 2 test of 3.973, RR for A2 = 1.02, for A28 = 1.97, for A32 = 1.25, for B5 = 1.44, for B8 = 1.27, for B13 = 1.91 without statistically significant differences of frequencies except for A1. Investigation of differences between haplotype frequencies among controls and patients showed statistically significant difference of A10 B40 haplotype in CML with RR value 7.24, while there were no statistically significant differences between controls and other leukemias. DISCUSSION: Our results of investigation showed statistically significant differences between HLA frequencies in the control group and investigated diseases, and that the relative risk is under 1, and values of chi 2 test under borderline values for B21 and B35 in AML, for B7 in ALL, for B15 in CML and for A1 in CLL. CONCLUSION: Results of this investigation point to an association between HLA system and leukemias. This association is of great importance because it provides a new tool for investigation of genetics and etiology of abovementioned diseases.

Adolescent↗

PubMedDate not supplied

[Distribution of HLA class II antigens and allele linkage disequilibrium in the population of Vojvodina].

INTRODUCTION: Polymorphism of human leucocyte antigens (HLA) is important in transplantation medicine, anthropological studies and paternity testing. MATERIAL AND METHODS: We investigated polymorphisms of HLA class II in population of Vojvodina by means of serologic typing using microlymphocitotoxicity test. We calculated the HLA-DR and HLA-DQ gene frequencies in 174 subjects. Haplotype frequencies, coefficient of linkage disequilibrium (delta values) and their statistical significant levels, were analyzed on the basis of these data. RESULTS: The most frequent HLA-DR and HLA-DQ alleles were: DR5(11) (gene frequency 0.138), DR4 (gene frequency 0.083), DR1 (gene frequency 0.077), DQ1 (gene frequency 0.388), DQ3 (gene frequency 0.197), and DQ2 (gene frequency 0.09). The highest positive values of coefficient of linkage disequilibrium (delta) were calculated for the following haplotypes: DR1DQ1 (delta value 0.07308708), DR2DQ1 (delta value 0.059846528), DR3DQ2 (delta value 0.06193263), DR2(15)DQ1 (delta value 0.039186022), DR5(11)DQ3(7) (delta value 0.04969439), DR7DQ2 (delta value 0.057985517). Significant differences between observed vs. expected haplotype frequencies were also considered for these haplotypes. CONCLUSION: This study indicates distinctiveness and specificity of the population of Vojvodina and highlights the importance of determining HLA frequencies in endogamic groups of Serbia.

Gene Frequency↗