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B Binder

Publications and source records attributed to B Binder.

At least 37 records · Page 2Linked to original sources
PubMed1991

[Oligosymptomatic variant of tuberous sclerosis with subependymal giant cell astrocytoma].

We report on a 9-year-old boy who developed subependymal giant-cell astrocytoma associated with tuberous sclerosis. Remarkably, the boy was not mentally retarded and never had suffered from seizures. Apart from white skin patches symptoms of increased intracranial pressure were the presenting symptoms. The boy's mother, who was present at the boy's admission, presented with skin lesions typical of tuberous sclerosis. The importance of thorough examination of apparently asymptomatic relatives of patients with tuberous sclerosis is emphasized.

Astrocytoma↗
PubMed1989

Sulfide induction of synthesis of a periplasmic protein in the cyanobacterium Oscillatoria limnetica.

Two proteins which may play a role in the induction of anoxygenic photosynthesis in Oscillatoria limnetica have been demonstrated by comparing the pattern of labeling during pulses of [35S]methionine of cells incubated under inducing conditions [anaerobic conditions plus 3-(3,4-dichlorophenyl)-1,1-dimethylurea, light, and sulfide) with that of cells incubated under noninducing conditions (without sulfide). The major inducible protein has an apparent molecular mass of 11.5 kilodaltons and is associated with a less strongly labeled 12.5-kilodalton protein. The synthesis of both proteins commences within the first 30 min of induction and continues throughout the 2-h induction period. Since these proteins are not synthesized in the presence of dithionite without sulfide, low redox potential alone is insufficient as an inducer of these proteins. Lysozyme treatment and/or osmotic shock of intact cells results in the release of the sulfide-induced proteins. Our data thus indicate that these proteins are located in the periplasmic space of the cells.

Amino Acids↗
PubMed1987

Ontogeny of adenosine 3',5'-monophosphate metabolism in rabbit cerebral cortex. Development of responses to histamine, norepinephrine, adenosine, and glutamate.

The effects of norepinephrine (NE), histamine (HIST), glutamate, and adenosine, singly and in combinations, on the accumulation of adenosine 3',5'-monophosphate (cyclic AMP) in slices of rabbit cerebral cortex were examined using tissue from animals 4 days before to 38 days after birth. A response to NE became visible 2 days before birth and increased to a maximum at 7 days after birth before declining toward the small adult value during the second post-natal week. During this period NE was at least twice as efficacious as isoproterenol, and both alpha- and beta-adrenergic antagonists had prominent inhibitory effects. Responses to HIST were already apparent 4 days before birth and increased in an irregular fashion thereafter, sometimes exceeding the adult value during the second post-natal week. The response to adenosine was not visible until birth and gradually increased toward the adult value during the entire period examined. Synergistic responses to various combinations of the three agents were first detected at 2 to 4 days before birth. The degree of synergism was larger during the neonatal period than that found in adult tissue; no synergism between HIST and adenosine persisted in the adult. During the first post-natal week, L-glutamate produced very large increases of cyclic AMP accumulation in the presence of either adenosine or histamine plus theophylline; smaller but substantial responses occurred in combination with NE. Responses to glutamate declined progressively after about the tenth post-natal day.

Adenosine↗
PubMed1987

Sulfide-dependent photosynthetic electron flow coupled to proton translocation in thylakoids of the cyanobacterium Oscillatoria limnetica.

Light-induced proton translocation coupled to sulfide-dependent electron transport has been studied in isolated thylakoids of the cyanobacterium Oscillatoria limnetica. The thylakoids are obtained by osmotic shock of washed spheroplasts, prepared with glycine-betaine as the osmotic stabilizer. 13C NMR studies suggests that betaine is the major osmoregulator in O. limnetica. Thylakoid preparations obtained from both sulfide-induced anoxygenic cells and noninduced oxygenic cells are capable of proton pumping coupled to phenazinemethosulfate-mediated cyclic electron flow. However, only in the induced thylakoids can sulfide-dependent proton gradient (delta pH) formation be measured, using either NADP or methyl viologen as the terminal acceptor. Sulfide-dependent delta pH formation correlates with a high-affinity electron donation site (apparent Km 44 microM at pH 7.9). This site is not lost upon washing of the thylakoids. In addition, both sulfide-dependent electron transport and delta pH formation are sensitive to inhibitors of the cytochrome b6f complex such as 2-n-nonyl-4-hydroxyquinoline-N-oxide, 2,4-dinitrophenyl ether of 2-iodo-4-nitrothymol, or stigmatellin. Sulfide-dependent NADP photoreduction of low affinity (which does not saturate by as much as 7 mM sulfide) is detected in both induced and noninduced thylakoids, but this activity is insensitive to the inhibitors and is not coupled to proton transport. It is suggested that the adaptation of O. limnetica to anoxygenic photosynthesis involves the induction of a thylakoid factor(s) which creates a high-affinity site for sulfide, and the transfer of its electrons via the cytochrome b6f complex, coupled to proton translocation.

Cyanobacteria↗
PubMed1977

[Zinc uptake of normal and leukaemic lymphocytes in an in vitro model (author's transl)].

Zinc uptake and/or possible exchange by isolated lymphocytes from normal persons and from patients with chronic lymphatic leukemia and chronic myeloid leukemia were studied in an in vitro model. The uptake of zinc per 10(9) lymphocytes of normal persons was 60 +/- 10 nanomoles and 120 +/- 15 nanomoles during incubation for 30 and 60 minutes, respectively. Two out of the 3 patients with chronic myeloid leukemia showed normal zinc uptake in this test system, whereas zinc uptake by lymphocytes from patients with chronic lymphatic leukemia was significantly decreased (22+/-8 nanomoles in 30 minutes and 35+/-10 nanomoles in 60 minutes).

Adult↗
PubMed1977

[Comparison of the efficacy of parenteral and enteral pertussis immunization in the rabbit (author's transl)].

The effects of immunization with inactivated B. pertussis were studied in the rabbit using enteral (oral and via suppositories) and parenteral immunization routes. The rise in agglutinating and complement-binding immunoglobulins in the rabbit serum was about equal following immunization by suppository and parenteral administration, but the demonstration of local immunity (immunoglobulins reacting with B. pertussis in salive) by immunofluorescence methods was observed only in the group immunized with enterally-administered B. pertussis.

Administration, Oral↗
PubMed1977

[Maximum exercise testing during hypobaria and normoxia (author's transl)].

The effects of high altitude (3000 m, low-pressure chamber) on maximum exercise and maximum oxygen uptake were studied in four normal subjects. In order to separate the effects of hypobaria and of hypoxia, exercise testing was performed under normoxic, hypobaric conditions and compared with the results under normoxic, normobaric conditions. There was no significant difference in performance, nor in the effects of exercise on the measured cardiovascular parameters.

Adult↗
PubMed1977

[Preparation of antilymphocytic globulin by means of repeatedly-used immunoadsorbents (author's tranl)].

A method is described for the purification of horse anti-human lymphocyte serum, whereby absorption of haemagglutinating antibodies was performed with human RBC ghosts. These ghosts were repeatedly used after desorption. Desorption was carried out in glycine-HCl buffer solution pH = 3,0. The advantages of this method consist of a reduction in the amount of RBC needed for absorption and the relatively low loss of lymphocytotoxic antibodies. The final product is effective in an in vitro test system and complies with the requirements for human use.

Animals↗
PubMed1977

[Effects of experimental hypovolaemic shock on platelets in the pig (author's transl)].

The effects of standardized blood loss on the platelets, as well as on coagulation, fibrinolysis and complement parameters were studied in the pig. A simultaneous fall in arterial and venous platelet counts was demonstrated during the initial phase of blood loss, with a concomitat rise in pulmonary vascular resistance. After exsanguination to the extent of about 40% of the blood volume, the arterial platelet counts increased significantly, whilst the pulmonary vascular resistance, which was negatively correlated with the arterial platelet counts throughout the experiment, fell. Venous platelet counts, on the other hand, remained low during this experimental period and were correlated with cardiac output, but not with pulmonary vascular resistance.

Animals↗