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Biomedical subjects

B Brozović

Publications and source records attributed to B Brozović.

At least 19 recordsLinked to original sources
PubMed1991

Effect of EDTA on platelet count and other platelet parameters in blood and blood components collected with CPDA-1.

We have shown in this study that addition of dried K2EDTA (1.5 mg/ml) to blood samples anticoagulated with CPDA-1 increases significantly the platelet count and mean platelet volume (MPV) of whole blood, red cell concentrate (RCC) and platelet concentrate (PC), but not of platelet-rich plasma (PRP) or of platelet-poor plasma (PPP). Transmission and scanning electron microscopy illustrated that platelet aggregates which are present in some components are dispersed on mixing of the sample with EDTA and that this is accompanied by a change in platelet morphology. Determination of the platelet distribution width (PDW) indicated that the platelet populations in whole blood and RCC seem to be more uniform in size than the populations in PRP, PPP and PC. The determination of MPV and PDW changes after addition of EDTA may provide a new approach to quality control of PC.

Adenine↗
PubMed1985

Lymphocytopenia in donors undergoing regular platelet apheresis with cell separators.

The effect of regular platelet apheresis on lymphocyte count was investigated in 142 platelet donors who donated up to 113 times on Haemonetics Model 30 and Fenwal Model CS-3000 cell separators. Lymphocytopenia (lymphocyte count less than 0.9 X 10(9)/l) was found in 21 out of 113 (18.6%) donors who donated solely on the Haemonetics Model 30 compared with 2 out of 100 (2%) whole blood donors and none of 100 plasmapheresis donors (P less than 0.002). Lymphocyte count was inversely related to the total number of platelet donations (r = 0.51, P less than 0.001) and total estimated lymphocyte loss (r = 0.49, P less than 0.001) suggesting a cumulative effect. T4 and T8 lymphocyte subsets were found to be reduced in each of 13 lymphocytopenic platelet donors tested but T4/T8 ratios were similar to those of controls (2.51 +/- 0.79 and 2.33 +/- 0.58 respectively). No adverse effects on platelet donor health were observed but it is suggested that platelet apheresis protocols should be designed to minimize lymphocyte loss.

Adult↗
PubMed1984

Preservation of platelet function in cryopreserved platelet concentrates with prostacyclin.

Prostacyclin (Epoprostenol) or a stable prostacyclin analogue (ZK 36,374) were added to platelet concentrates prior to cryopreservation. This resulted in significantly better preserved function of the thawed platelet concentrate, assessed by platelet aggregation to various concentrations of ADP, collagen and ristocetin, compared to control cryopreserved platelet concentrates. The use of prostacyclin or one of its stable analogues should be considered to reduce platelet activation and subsequent loss of function during the various manipulative procedures when preparing standard and cryopreserved platelet concentrates.

Blood Platelets↗
PubMed1982

Leucocyte-depleted blood: a comparison of cell-washing techniques.

Non-haemolytic febrile transfusion reactions are common in multi-transfused patients. It is generally accepted that the majority of these reactions occur when more than 0.5 x 10(9) leucocytes are transfused. Values equal to or lower than this threshold, which we have called for convenience the critical antigenic load for leucocytes (CALL), can be achieved by decreasing the leucocyte content in one unit of whole stored blood by about 80%. We have compared the efficiency of leucocyte depletion of whole stored blood, using fixed-speed centrifugation (Haemonetics Model 15 and Model 115 cell washer), variable-speed centrifugation (Dideco Progress 90 Cell Separator), and serial centrifugation (IBM 2991 Blood Cell Processor. Fixed-speed centrifugation was least effective in depletion leucocytes; a reduction of 25 and 60% was achieved using the Haemonetics Model 15 and Model 115, respectively. Variable speed and serial centrifugation produced more satisfactory results with leucocyte depletions of 82 and 89% using the Dideco Progress 90 Cell Separator and the IBM 2991 Blood Cell Processor, respectively. Platelet depletion of over 90% was achieved with all the cell washers, except the Haemonetics Model 15. Red cell losses varied from 3 to 30%. It seems unlikely that consistently high (over 90%) leucocyte depletion can be obtained using cell-washing techniques without associated high red cell losses.

Blood Platelets↗
PubMed1981

Buffy coat and cell separator granulocyte concentrates. Comparison of cell content and in vitro granulocyte function.

Buffy coat granulocyte concentrates were prepared manually from single fresh whole blood donations after centrifugation. The erythrocyte, granulocyte and platelet numbers in these concentrates were compared to cell numbers in concentrates obtained by mechanical leukapheresis using intermittent flow centrifugation. Buffy coats contained approximately half the number of granulocytes and three times the number of erythrocytes per unit volume than cell separator concentrates. There was no difference in platelet numbers. Thus to give a therapeutic dose of granulocytes equivalent to that obtained by cell separation, 2-3 times greater volume of buffy coat concentrates would be required. In vitro granulocyte function, as measured by migration, phagocytosis, and Candida killing was also compared in buffy coat and cell separator concentrates. The only difference found was reduced Candida killing in the buffy coat granulocytes. The possible clinical use of buffy coat concentrates, as a source of granulocytes, is discussed.

Blood Cell Count↗
PubMed1976

An improved automated method for the measurement of red cell 2,3-diphosphoglycerate.

A modified automated colorimetric micromethod for the determination of red cell 2,3-diphosphoglycerate (2,3-DPG) adapted from that of Grisolia et al (1969) is described. In the modified method, ethylenediaminetetra-acetic acid (EDTA) is not used and consequently concentrations of several reagents are changed. During the development of the method it was found that the presence of EDTA, either in the blood or in reagents, consistently reduced the measured value of 2,3-DPG by 15%. This effect of EDTA, not previously recognized, is independent of the EDTA concentration within the range of 5 to 50 mmol/1 and is at present unexplianed. In normal subjects (41 men and 30 women) the mean red cell 2,3-DPG was 14-5 mol/g haemoglobin (range 12-1-18-1 mol/g haemoglobin). There was no significant difference in 2,3-DPG concentrations between male and female subjects.

Adolescent↗
PubMed1975

A simple automated micromethod for measuring serum copper.

An automated micromethod for measuring copper concentration in 0.2 ml of serum employing formaldehyde-oxalydihydrazide as chromogen and using an Auto-Analyzer (Technicon) is presented. The use of formaldehyde-oxalyldihydrazide resulted in a stable and noise-free baseline. The coefficient of variation of the micromethod was 2.4%. The recovery of copper added to samples was 102%. A good correlation between the serum values estimated by the described method and the manual method of Summers (1) (r = 0.929; P less than 0.001) was found when copper standards were prepared in 0.277 M glucose. The mean serum copper concentration found in 26 healthy adult males is 94.1 mug/dl (range: 80 to 120 mug/dl) and in 20 females is 109.8 mug/dl (range: 71 to 170 mug/dl), the difference being statistically significnt (P less than 0.02).

Adult↗
PubMed1975

Haematological status of middle- and long-distance runners.

1. Haematological investigation and blood volume measurements were carried out on forty male middle and long distance runners and twelve non-athletes. 2. The distribution of haemoglobin concentration, packed cell volume, erythrocyte count, total ironbinding capacity, serum and erythrocyte folate and serum vitamin B12 concentrations were essentially the same in atheletes and non-athletes. The mean serum iron concentration was higher in non-athletes than in athletes. There was no difference in the above measurements between athletes taking iron and/or folate and athletes not taking these supplements. 3. Blood volume and total body haemoglogin were on average 20% higher in the atheletes than in the non-athletes. 4. There was no correlation between haemoglobin concentration and blood volume in athletes. The evidence of this study suggests that haemoglobin concentration and blood volume are independently controlled. 5. 2,3-Diphosphoglycerate concentration in the erythrocytes was higher in the athletes than in the non-athletes; the mean values were 15-9 and 14-2 mumol/g of haemoglobin respectively.

Adult↗
PubMed1974

An automated micromethod for measuring iron concentration in serum using thioglycollic acid and bathophenantroline sulphonate.

An automated micromethod for measuring iron concentration in serum employing thioglycollic acid and bathophenantroline sulphonate as reducing agent and chromogen respectively, is described. Measurements are carried out using an AutoAnalyzer (Technicon) and require 0.1 ml of sample. The reproducibility, assessed by the mean coefficient of variation (1.9%), and the mean recovery of iron added to samples (99.5%), as well as the correlation between the serum iron values estimated by the described method and the method recommended by the Expert Panel on Iron of the International Committee for Standardization in Haematology (r = 0.9779; p < 0.001; y = 0.9713 x -0.0546), is highly satisfactory. The method can also be used for measuring total ironbinding capacity of serum. The method appears equally suitable for routine work and research studies when a large number of samples and a small volume of serum are available.

Autoanalysis↗
PubMed1973

The diagnosis of iron deficiency in patients with Crohn's disease.

Red cell indices were determined and bone marrow was examined in a selected group of 21 patients with Crohn's disease who had a routine peripheral blood picture suggestive of iron deficiency. Only nine (43%) of these patients had no stainable iron stores in the marrow fragments and could be considered as being definitely iron deficient. All indirect measurements in the diagnosis of iron deficiency, except the total iron-binding capacity (TIBC), appeared likely to result in over-diagnosis. Iron-deficient erythropoiesis, without true deficiency, may be due to the inflammatory disease process and this study indicates that the examination of bone marrow aspirate is necessary for the certain diagnosis of iron deficiency in Crohn's disease.

Adolescent↗