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Biomedical subjects

B Brun

Publications and source records attributed to B Brun.

137 records · Page 8Linked to original sources
PubMedDate not supplied

Studies on the origin and nature of seminal proteins by cellulose acetate electrophoresis.

Electrophoresis of semen on cellulose acetate strips allowed the localization of 19 protein bands. Twelve of these were analyzed for their composition and ten for their tissue of origin. Four bands were composed of prostatic products, three contained secretions form the seminal vesicles, and various serum products composed six others. Assays were also performed for acid phosphatase activity, glycoproteins and proteins bound to zinc. Proteins in semen were found to be broken down with time during incubation at 37C and therefore only fresh specimens or material which is frozen immediately after liquefaction may be used with these techniques. Our experiments clearly show that electrophoresis of semen may be a useful technique in clinical andrology: in particular in the case of excretory azoospermia and infections of the genital tract.

Acid Phosphatase↗
PubMedDate not supplied

Comparative electrophoretic study between rabbit and human seminal fluid.

Cellulose acetate electrophoresis of rabbit seminal fluid give, after amidoschwartz staining, 19 protein bands, all migration towards the anode. Nine of them are present in at least 80% of the samples. With the acid of specific detection techniques, it was possible to demonstrate th presence of zinc in four bands, acid phosphatase activity in two and glycoprotein in three of the bands. Serum compounds seem to be present in small quantities. Study of fluids squeezed out from prostate, paraprostate, seminal vesicle and epididymal tail show few specific bands in these secretions: four in the case of prostate, three for paraprostate and one for seminal vesicle. Comparisons between human and rabbit seminal fluid electrophoresis show no relationship between these two types of secretion.

Acid Phosphatase↗
PubMedDate not supplied

Evaluation of the cerebral vasodilatory capacity by the acetazolamide test before EC-IC bypass surgery in patients with occlusion of the internal carotid artery.

Cerebral blood flow (CBF) was measured by xenon-133 inhalation tomography in 18 patients with cerebrovascular disease before and 4 months after extracranial-intracranial bypass surgery. Only patients who showed a reduced CBF in areas that were intact on the CT scan and relevant to the clinical and angiographical findings were operated. The majority of the patients had suffered a minor stroke with or without subsequent transient ischemic attacks. They were studied at least 6 weeks following the stroke. All patients had an occlusion of the relevant internal carotid artery. To identify preoperatively the patients with a compromised collateral circulation and hence reduced CBF due to reduced perfusion pressure, a cerebral vasodilatory stress test was performed using acetazolamide (Diamox). In normal subjects, Diamox has been shown to increase tomographic CBF without change of the flow distribution. In the present series 9 patients showed a significant redistribution of flow in favor of the non-occluded side ("positive" Diamox test). Two of these 9 patients showed even a paradoxical decrease in focal CBF preoperatively, i.e., a "steal" effect. These 2 patients were the only patients who improved in focal CBF after shunting. The remaining 9 patients all showed uniform flow responses ("negative" Diamox test), and none of these increased in focal CBF postoperatively. The finding of an unchanged flow map postoperatively confirmed that the low flow areas were not due to restricted flow via collateral pathways. However, an increase in the regional vasodilatory capacity was observed postoperatively in the majority of patients.

Acetazolamide↗
PubMedDate not supplied

Silent cerebral infarction in chronic atrial fibrillation.

Atrial fibrillation (AF) is associated with an increased risk of stroke. In AF patients with acute stroke, cerebral computed tomography (CT) often reveals old asymptomatic infarcts. To investigate the frequency of such lesions, 29 AF patients and 29 controls in sinus rhythm without history of cerebrovascular disease were CT scanned. Fourteen patients with AF (48%) had abnormal CT scans with areas of low density with sharp demarcation from surrounding tissue compared with 8 patients in sinus rhythm (28%) (p greater than 0.10). However, the number of abnormal areas with apparent tissue loss was significantly higher in the AF group (39 lesions) compared with the control group (16 lesions) (p = 0.033). The lesions were mainly located in the cortex with no significant difference in lesion size between AF patients and controls. The abnormal areas probably reflected small, clinically silent infarcts. We conclude that these lesions are present in AF patients without history of cerebrovascular events and occur more frequently than in controls without atrial fibrillation.

Aged↗
PubMedDate not supplied

Cerebral computed tomography in men with acquired immunodeficiency syndrome.

Cerebral CT scannings were performed in 19 homosexual men with the acquired immunodeficiency syndrome (AIDS). Nearly half of them (9 patients) had cortical atrophy. Three patients with toxoplasmosis had cerebral pathology, in two of them with ring enhancement while the third had an ill-defined nonspecific lesion with slight heterogeneous enhancement without ring formation. Two patients with multifocal leucoencephalopathy and non-Hodgkin's lymphoma, respectively, presented non-enhancing, low attenuating lesions at CT.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Effectiveness of hemodialysis in a case of acute methotrexate poisoning].

In a case of acute intoxication with high-dose methotrexate, a kinetic study of plasma methotrexate concentrations enabled the authors to begin treatment with high-permeability membrane haemodialysis combined with intensive folic acid loading before the clinical signs of iatrogenic toxicities developed, and to continue with haemodialysis rather than using other depurative methods. In this case, the post-depuration course was favourable: dermatological signs and febrile pancytopenia regressed within 4 days, and these was no sign of hepatic toxicity.

Acute Kidney Injury↗
PubMedDate not supplied

[Morphologic acrosomal changes in a man exposed to heat].

In view of a case of primary sterility with heat professional exposure and abuse of warm baths, we have studied the spermatozoa ultrastructural morphology. Same type of acrosomal malformations were observed in spermatozoa following experimental scrotal heating. These abnormalities appears to produce sterility in this case.

Acrosome↗
PubMedDate not supplied

[Acute renal failure induced by retroperitoneal fibrosis revealing Hodgkin's disease. A case report. Five years complete remission with surgical and medical treatment. Subsequent fatal bronchogenic carcinoma (author's transl)].

A 57 year-old-man with acute renal failure was diagnosed as having retroperitoneal fibrosis. Malignant lymphoma is suspected because of peripheral lymphadenopathy, but biopsies are unrevealant. Nephrostomy restores kidney function. Many other histologic samplings performed during three laparotomies are necessary to diagnose Hodgkin's disease. Nephrostomy is complicated with iterative acute pyelonephritis and septicaemias which make chemotherapy unadvisable. Ureterolysis is unsuccessful. Urinary tract infection disappears after uretero-ileoplasty. Subsequently chemotherapy induces a complete remission during 5 years with normal renal function. We review two previously reported cases of this unusual cause of malignant retroperitoneal fibrosis. Diagnostic procedures and therapeutic management are discussed.

Acute Kidney Injury↗