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Biomedical subjects

B C Deer

Publications and source records attributed to B C Deer.

5 recordsLinked to original sources
PubMed1982

Salicylate ototoxicity in the chinchilla: a behavioral and electron microscope study.

Normal audiograms were obtained from a group of behaviorally conditioned chinchillas. Following this the animals were injected with sodium salicylate. Temporary hearing losses were measured 1 1/2 hours after treatment. Animals were anesthetized and blood samples taken to determine salicylate levels. The animals were then decapitated and the temporal bones were examined by SEM and TEM. No morphological correlate was seen for temporary hearing losses which were generally in the neighborhood of 30-40 dB.

Animals↗
PubMed1980

Method for staining and washing thin sections on support films.

A procedure is described for staining large numbers of thin sections on support films for use with one-hole grids. The film is picked up, carried and protected using easily made plastic blocks. Loop-tipped forceps are then used to transfer tissue ribbons from the knife boat to the support film. A large number of tissue sections can then be stained and washed simultaneously in a modified Pyrex dish without damaging the film. After staining, the slot in the one-hole grid is centered over the tissue ribbon, and the grid is attached to the film. The method is suitable for serial reconstruction and the unobstructed viewing of large thin sections in the TEM.

Histological Techniques↗