Enzyme histochemistry of induced heterotropic bone formation in guinea-pigs.
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Acid Phosphatase↗
Biomedical subjects
B C Magnusson
Publications and source records attributed to B C Magnusson.
Ameloblastic fibroma.
Four cases of ameloblastic fibroma appearing in the posterior maxilla and the cupsid area of the mandibula are presented. One of the lesions appeared in the wall of a cyst and another was found in connection with a cyst. The treatment is conservative but the tumor needs close follow up for possible recurrence.
Adolescent↗
Odontogenic keratocysts: a clinical and histological study with special reference to enzyme histochemistry.
Of a total of 1,420 odontogenic cysts, 52 (3.3%) were diagnosed as odontogenic keratocysts. Clinical and histological findings in these 52 cysts are reported. Frozen sections of 26 of the keratocysts were incubated to show the following enzyme activities: NADH2- and NADPH2-diaphorase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, acid phosphatase, leucine aminopeptidase and ATPase. Furthermore, keratinization was studied with the rhodamine B method and lipids with the oil red O, the OTAN and the acid hematein methods. Sections from epidermis, oral mucosa, radicular cysts, residual cysts and follicular cysts served as reference material. The oxidative enzymes showed strong activity in the keratocyst epithelium which contrasted with weak activity in the reference cysts. Acid phosphatase activity was weak in all epithelia except that in keratocysts, which displayed a marked activity. In the fibrous capsule of the keratocyst a high activity of leucine aminopeptidase was recorded. This high activity contrasted with a weak activity in the reference material. The significance of the histochemical results in relation to the aggressive behavior of the keratocyst is discussed.
Acid Phosphatase↗
Sutural closure in rabbit and man: a morphological and histochemical study.
Some aspects of normal obliteration of sutures in rabbit and man were studied histologically and it was found that suture closure took place by intramembranous ossification in specific areas of trans-suturally arranged, tendon-like tissue. Histochemically these areas demonstrated high activity of oxidative enzymes.
Adolescent↗
Odontoblast metabolism in rats deficient in vitamin D and calcium I: A histochemical survey.
Different rat diets, deficient in calcium and/or vitamin D, were tested for varying experimental periods to obtain changes in serum calcium values, body weight gain and odontoblast-predentine morphology. One diet, R 25, used during a 14-day period, was found to induce lowered serum calcium values and an increased predentin width in incisor teeth. Rats fed this diet demonstrated an increase in alkaline phosphatase and ATP-splitting enzyme activity in dentinogenically active incisor odontoblasts. No other metabolic changes in these cells were demonstrated by the histochemical methods employed.
Animals↗
Ca 2+-ATPase in hard tissue forming cells.
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Adenosine Triphosphatases↗
Inhibition studies of alkaline phosphatase in hard tissue-forming cells.
The effects of the alkaline phosphatase inhibitors levamisole and R 8231 on p-nitro-phenylphosphatase, inorganic pyrophosphatase and adenosine triphosphatase (ATPase) activities in dentingenically active odontoblasts were studied. The p-nitrophenylphosphatase and inorganic pyrophosphatase activities were inhibited, while 40% of the ATP-splitting enzyme activity remained under the assay condition used. This finding, togeather with earlier studies, indicates that at least two different phosphatase are active at alkaline pH in hard tissue-forming cells; on nonspecific alkaline phosphatase and one specific ATPase. The ATPase activity is uninfluenced by ouabain and ruthenium red and is activated by Ca-2+ ions.
4-Nitrophenylphosphatase↗
Inorganic pyrophosphatase in isolated enamel organ and odontoblasts from the rat incisor.
The inorganic pyrophosphatase (PPiase) activity was determined by a colorimetric method in the odontoblasts and the parts of the enamel organ related to enamel matrix formation and enamel maturation. The effects on PPi hydrolysis by EDTA, R 8231, urea and heat treatment were found to be almost identical to those reported for nonspecific alkaline phosphatase (APase) in the same tissues. The Mg2+ activation curve for PPiase was also similar. Like those of APase, these characteristics of PPiase activity were identical in the three locations studied. It is suggested that the close similarity in the properties of PPiase and APase is due to activity of the same enzyme, a concept which is in agreement with recent biochemical and histochemical studies of calcification.
Animals↗