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B Chaudhary

Publications and source records attributed to B Chaudhary.

12 recordsLinked to original sources
PubMed2003

Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR).

In Agrobacterium-mediated genetic transformation of cotton (Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S gus-int fragment as a reporter to optimize the transformation protocol. Although the concentration of kanamycin was reduced during embryogenesis and embryo maturation, even at the lower levels somatic embryos were predominantly abnormal, showing hypertrophy and reduced or fused cotyledons or poor radicle ends. A majority of these embryos (more than 75%) were beta-glucuronidase (GUS)-positive. Embryos with an abnormal appearance showed a very poor conversion to plantlets. However, these embryos, when subjected to slow physical desiccation followed by transfer to fresh medium, regenerated single or multiple shoots from the cotyledonary end. These shoots could be grafted on wild-type seedling stocks in vitro, which, following their transfer to soil, developed normally and set seeds. Regenerated plants tested positive for the transgene by Southern analysis. An overall scheme for the high-frequency production of cotton transgenics from both normal and abnormal appearing somatic embryos is presented.

Cells, Cultured↗
PubMed2001

Sleep disorders: a risk factor for pseudotumor cerebri?

OBJECTIVE: To determine whether sleep-related breathing disorders are common in patients with idiopathic intracranial hypertension. MATERIALS AND METHODS: Medical records of 53 patients with idiopathic intracranial hypertension from a tertiary center neuroophthalmology practice were reviewed. Thirty-seven patients were identified who had a history of snoring, difficulty sleeping, or daytime somnolence. The data from polysomnograms were tabulated to determine the frequency of apneas, hypopneas, and arousals. RESULTS: Fourteen of 37 patients with idiopathic intracranial hypertension and symptoms of sleep disturbance underwent polysomnography. There were two men and 12 women varying in age from 24 to 58 years (mean, 39.4 +/- 11.9). These patients were obese with body mass indexes varying from 33.0 to 63.2 (mean, 46.0 +/- 9.5). A diagnosis of sleep apnea was made in six and upper airway resistance syndrome in seven patients. CONCLUSIONS: Sleep-related breathing problems were common in our patients with idiopathic intracranial hypertension. Obesity was common in these patients and may be playing a causative role in sleep apnea and idiopathic intracranial hypertension. It is suggested that idiopathic intracranial hypertension patients who have symptoms of sleep disturbance should be further evaluated for the presence of sleep-related breathing problems.

Adult↗
PubMed2001

Attention deficit hyperactivity disorder in adults and obstructive sleep apnea.

Attention deficit hyperactivity disorder (ADHD) is a common childhood illness with a prevalence between 3% and 16%. It is characterized by hyperactivity, impulsiveness, impairment in academic, social, and occupational functioning, short attention span, and onset of symptoms before age 7 years. In some patients, this illness can persist into adulthood. Obstructive sleep apnea (OSA) is a common disorder affecting 3% of the population and is characterized by hypersomnolence, snoring, disturbed sleep, and cognitive dysfunction. Some studies have suggested an association between OSA and ADHD in children. Because of the overlap of symptoms, patients with OSA may be considered to have ADHD. Significant improvement in symptoms has been reported with the use of continuous positive airway pressure in patients with OSA. We present reports of three adults who were being treated for ADHD and were found to have OSA.

Adult↗
PubMed1998

Hour-to-hour variability of oxygen saturation in sleep apnea.

STUDY OBJECTIVES: Methods used to express the severity of oxygen desaturation during polysomnography include the average oxygen saturation (AO2), lowest oxygen saturation (LO2), and the percent of the total time with oxygen saturation level lower than 90% (T<90%). We wanted to determine which one of these methods is least variable during different hours of monitoring. DESIGN: Prospective, observational study. SETTING: Sleep center at a medical university. PATIENTS: One hundred fifty patients with apnea-hypopnea index from 5 to 130. MEASUREMENTS: AO2, LO2, and T<90% were calculated during each of the 8 h of polysomnography. Data for each hour were compared and the Cronbach alpha coefficients were calculated. RESULTS: There was a high degree of correlation among the three methods as well as between each method and the severity of sleep apnea. The mean+/-SD values for each method were as follows: AO2, 92.7+/-5.6; LO2, 68.5+/-19.3; and T<90%, 15.7+/-24.2. The alpha coefficients for these methods were AO2, 0.98; LO2, 0.88; and T<90%, 0.98. In all methods, the data of the first hour were significantly different from the data of the subsequent hours. CONCLUSION: Both AO2 and T<90% methods show less hour to hour variability compared with LO2, and there is more variability in the first hour. Since the AO2 values >90% may not convey the severity of O2 desaturation, T<90% may be the best method of expressing oxygen saturation changes during polysomnography.

Female↗
PubMed1996

Suthi feeding.

Explore the source record for details and available documents.

Cooking and Eating Utensils↗
PubMed1989

Sleep apnea, sleep disorders, and hypothyroidism.

The symptoms of hypothyroidism are protean and include apathy, somnolence, lethargy, personality change, and intellectual deterioration. Many of these symptoms may be related to hypothyroid-induced sleep disorders. Hypothyroidism is associated with abnormal ventilatory drive, abnormal sleep architecture, and sleep apnea. Central, obstructive, and mixed patterns of sleep apnea are commonly observed in hypothyroidism. A case of severe sleep apnea in a grossly myxedematous patient who improved dramatically following thyroid replacement alone is presented. Myxedema is a reversible cause of sleep apnea, and thyroid function testing should be considered in its diagnostic work-up.

Adult↗
PubMed1989

Sleep apnea: morbidity and mortality of surgical treatment.

We have analyzed the complications in 132 patients who had surgical treatment of sleep apnea or excessive snoring. In this series there were 34 (26%) complications and two patients (1.5%) died. Complications related to uvulopalatopharyngoplasty in 126 patients included rhinolalia (2%) and oropharyngeal hemorrhage (6%). In the 41 patients who had tracheostomy, complications included hemorrhage from tracheostomy (5%), tracheomalacia (5%), peristomal infection (15%), tracheostomy tube intolerance (Montgomery tube 100%, Shiley tube 7%), pneumonia (2%), hypoxia necessitating emergency tracheotomy (2%), and other perioperative complications.

Adult↗
PubMedDate not supplied

Recurrent giant cell reparative granuloma of hard palate: role of Tc-99m-MDP three-phase bone scan.

A 42-year-old female presented with pain in the hard palate following surgical curettage for giant cell reparative granuloma. Radiographs of the face at this time did not reveal any abnormality. The surgeons' query was to differentiate fibrosis from recurrence before considering her for recurettage. The three-phase bone scan helped in establishing the diagnosis of recurrence, which was confirmed after histological examination of the curettaged tissue.

Adult↗