Biomedical subjects
Publications and source records attributed to B Chaudhuri.
Light and electron microscopic studies of a benign, well circumscribed lipoblastoma in a 10-year-old boy were performed. Ultrastructural examination demonstrated lipocytes, lipoblasts, undifferentiated mesenchymal cells, and occasional hibernoma cell types. Myxoid liposarcoma as a differential diagnosis was considered. However, an absence of mitosis and the hyperchromatic and bizarre nuclei of the lipoblasts ruled out the possiblity of malignancy.
P53 aberrant protein expression and mutation is a component of many human tumors but less information is available regarding involvement in relevant animal models. We have examined invasive mammary epithelial neoplasms for p53 aberrant protein expression from Sprague-Dawley rats induced by two intrajugular injections of N-methyl-N-nitrosourea (NMU, 50 mg/kg), 7 days apart, beginning at 44-49 days of age. Positive nuclear staining was observed in 8/10 mammary tumor frozen sections using PAb 421, a mouse monoclonal antibody, compared to a mixed mouse IgG negative control antibody. Paraffin sections from two additional sets of similarly induced rat mammary tumors also showed positive nuclear staining (22/37 tumors) with CM-5, a rabbit polyclonal antibody. Our results indicate that elevated cellular content of p53 is a common event in invasive palpable mammary tumors induced by NMU in this dual-injection model system.
This case report is of an immunosuppressed patient in whom a diagnosis of disseminated Strongyloides infestation was made by cytologic examination of sputum, bronchial washing and brushing although a negative bronchial biopsy was reported. For those patients receiving steroids or immunosuppressives, the important role of cytology in the diagnosis of Strongyloides stercoralis is emphasized. The morphologic characteristics of this adult intestinal nematode and the differential diagnosis are also described.