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Biomedical subjects

B Christensson

Publications and source records attributed to B Christensson.

At least 163 records · Page 9Linked to original sources
PubMed1983

Correlation of immunophenotype to morphology in unfavourable non-Hodgkin lymphoma.

The relationship between immunological markers and histology according to the Kiel classification was studied in 40 adult non-Hodgkin lymphoma patients of Rappaport unfavourable histology. The membrane-associated and cytoplasmic Ig as well as receptors for sheep erythrocytes, Fc gamma and C3d receptors were analyzed on cryostate sections and in suspension. In some cases, a more precise immunophenotype was achieved by the use of monoclonal antibodies detecting different T and B cell antigens. Eighty-eight per cent of the lymphomas had B-cell, five per cent T cell and 7 per cent non-B/non-T cell phenotypes. All CBCC and CC lymphomas expressed monotypic Ig, but only 66 per cent of the CB lymphomas. Thus, morphology alone did not consistently predict immunophenotype in large-cell lymphomas. A simultaneous expression of multiple heavy chains and cytoplasmic Ig was found in some lymphomas, suggesting an intratumoral differentiation. The nodular or irregular tissue distribution patterns for Ig and C3d receptors found in histologically diffuse follicle derived lymphomas also suggest intratumoral variations in the marker expression, probably related to differentiation. The results suggest that lymphoma immunophenotype is important in obtaining a definite diagnosis in large-cell lymphomas and that it may lead to a better understanding of the differentiation of lymphomas of low-grade malignancy.

Adult↗
PubMed1983

A clinico-pathological and immunological study of unfavourable non-Hodgkin lymphomas. Comparison of the Rappaport and Kiel classifications.

To evaluate the prognostic information of the Kiel classification a homogeneous material of 63 non-Hodgkin lymphomas of unfavourable Rappaport histology were re-evaluated according to the definitions of the Kiel classification. The patients were selected from a prospective lymphoma study including 775 patients. Only ambiguous histological diagnoses analysed independently by two hematopathologists were accepted. Immunological markers of the tumours studied both in suspensions and on cryostate sections were in addition analysed in 40 of the patients. Forty-one per cent (26/63) were of high grade malignancy according to the Kiel classification, 59% (36/63) were of low-grade malignancy. The DLPD group was most heterogeneous while a better concordance was found between DM and CB/CC and between DH and CB cases. However, prognostic subgroups of the two classifications were only partly equivalent. A good correlation was found between the Kiel high-grade malignant group and patients of Rappaport poorest prognosis (DU, DH). Eighty-eight per cent of the lymphomas were of B cell, 5% of T cell and 7% of non-B-non-T phenotypes. Both the Kiel and Rappaport morphologic classifications predicted survival in this selected material. Patients with B phenotypes survived longer than patients with lymphomas of non-B type. Among patients with diffuse lymphomas, those with a nodular and irregular distribution of immunoglobulin and C3d receptors tended to respond better and survived longer. No prognostic information was obtained from immunoglobulin isotypes, C3d or Fc-gamma receptors. It is concluded that the Kiel classification is equally reliable for clinical judgement as the Rappaport system and that immunological marker studies may add prognostic information.

Adult↗
PubMed1983

The clinical significance of serological methods in the diagnosis of staphylococcal septicaemia and endocarditis.

In comparison to healthy controls we have found that patients with endocarditis and septicaemia caused by Staphylococcus aureus show significantly higher antibody levels against either S. aureus peptidoglycan, crude staphylococcal antigen or alpha-toxin. The serological methods used in these studies were radio-immunoassays. These results have now been further evaluated concerning their clinical significance. The occurrence rate of S. aureus endocarditis and septicaemia at the clinic was 0.36% and 2.0%, respectively, during the study period. In spite of relatively high levels of test specificities, 93.8-96.9%, the predictive values for positive test results were low, 2.7-23.2%. Using the PG-assay, satisfactory predictive values of 100% together with a sensitivity of 95.2% could nevertheless be reached in the screening for S. aureus endocarditis, if the upper normal limit was raised.

Antibodies, Bacterial↗
PubMed1983

Staphylococcus aureus septicaemia and endocarditis at the University Hospital in Lund 1976-1980.

During the period 1976-1980 Staphylococcus aureus was found in 265 blood cultures from 13 clinics for adult patients at Lund Hospital. Criteria of septicaemia were fulfilled in 169 patients, 65 had transient bacteremia and 31 cases were not evaluable. Concerning bacteriological data no Staphylococcus phage type dominated and strains resistant to antibiotics other than penicillin were very few. On the average 74% of the strains produced penicillinase with a successive increase during the study period. The incidence of septicaemia was highest in the decade 61-70 years of age. In patients with hospital-acquired septicaemia (n = 99) the main portals of entry for infection were vascular and/or surgical wounds (confirmed by phage typing in 93%). Patients with community-acquired septicaemia (n = 70) often had skin lesions but only a few cultures were taken. Only 4 patients were drug addicts. In 28 patients with no obvious portal of entry 14 nasal cultures were performed. Eight of ten positive cultures showed the same strain in nares as in blood at onset of sepsis. Secondary infectious foci were most frequent (26%) in patients with community-acquired infection. Endocarditis were found in 19 patients, 11 were diagnosed at autopsy. In staphylococcal endocarditis the mortality was 68% compared to 10% in septicaemia without endocarditis. In septic shock, compromised hosts and in connection with chronic diseases the mortality rates were 39%, 26% and 29%, respectively.

Adolescent↗
PubMed1982

Detection of Staphylococcus aureus antibodies in patients with S. aureus infections and in normal persons, using solid phase radioimmunoassay.

One hundred and thirteen patients with S. aureus infections, eight patients with non-S. aureus septicemia and 167 normal controls were investigated by solid-phase radioimmunoassay for staphylococcal antibodies. All serum samples tested had measurable antibodies, including the normal controls. The test could differentiate the patients group with S. aureus endocarditis from patients with other S. aureus septicemia, as well as from normal controls, as the endocarditis group had significantly higher antibody levels. Patients with non-bacteremic S. aureus infections, such as osteomyelitis and recurrent furunculosis, showed a wide range of antibody levels, 1/3 and 1/4 of the patients, respectively, showing high levels comparable to the endocarditis patients. Among normal controls, high antibody levels were found in 13 per cent.

Antibodies, Bacterial↗
PubMed1982

Staphylococcus aureus antibodies in patients with staphylococcal septicemia. Comparisons between solid-phase radioimmunoassay and crossed immunoelectrophoresis.

Antibody responses against teichoic acids and other antigens from Staphylococcus aureus in 23 patients with septicemia and 8 patients with endocarditis caused by S. aureus were investigated. Both methods used--solid-phase radioimmunoassay (SPRIA) and crossed immunoelectrophoresis (CIE)--detected significantly higher titres in the endocarditis group. The SPRIA method utilized a sonicated extract of S. aureus, strain Wood 46 for the quantitation of antibodies. In CIE precipitating anti-teichoic acid antibodies were measured. There was no absolute correlation between the two assays, indicating that the antibodies measured were directed at partly different antigens. The two tests might be complementary to each other when used in combination.

Antibodies, Bacterial↗
PubMed1981

An EBV-associated, Swedish case of Burkitt-type malignant lymphoma.

Morphological, serological, DNA-homology and immunofluorescence studies of a rapidly fatal malignant lymphoma in a 25-year-old Sweden male revealed most of the characteristics of an EBV-associated Burkitt lymphoma. The various findings are discussed in relation to previously reported observations on endemic (African) and non-endemic Burkitt lymphomas.

Adult↗
PubMed1980

Immunological studies on human thymus. Occurrence and distribution of immunoglobulins and immunological receptors in myasthenia gravis and control patients.

Suspended cells and tissue sections from myasthenia gravis (MG) and control thymuses were characterized for surface expression and histological distribution of immunoglobulin (Ig) and receptors for sheep erythrocytes (SE), the Fc part of IgG (Fc gamma) and of IgM (Fc mu) and complement factors C3b and C3d (CR). In sections the cortical areas from both MG and control thymuses expressed SE as well as Fc gamma receptors, whereas medullary areas only showed weak SE binding. In contrast to control patients, MG thymuses also contained Ig+ cells confined to nodular areas of the thymic medulla. These nodules stained for both IgG and IgM, and were polyclonal with respect to light chains. In several cases IgD was found as well. In addition, the nodular areas were positive for C3b and C3d receptors. In serial sections, these Ig+ CR+ nodules corresponded to morphologically characteristic lymphoid follicles. Such B cell follicles were observed in variable numbers in all MG thymuses, whether hyperplastic or with normal histology. Five thymomas gave heterogeneous results with regard to immunological markers. Comparison of methods showed that receptor and Ig indication on sections was more sensitive than corresponding tests on thymus cells in suspension.

Adult↗
PubMed1980

An immunohistological study of human lymphoma.

In this study the problems encountered in staining immunoglobulin (Ig) in sections of paraffin-embedded human lymphoma samples have been investigated. It was found that the "masking' of cytoplasmic Ig, which occurs when tissues are fixed in formol saline (the fixative employed in most previous studies), can be avoided by the use of mercury-based fixatives. When non-Hodgkin's lymphoma samples fixed in this way were studied it was found that cytoplasmic Ig labelling of both lymphoid and histiocytic cells is often attributable to non-specific uptake of serum proteins. This phenomenon probably accounts for a number of published anomalous immunoperoxidase staining results in human lymphoma (e.g. the presence of both kappa and lambda chains in the same neoplastic cell). Double immunoenzymatic labelling (using alkaline phosphatase and peroxidase) proved valuable in the elucidation of this phenomenon. When staining due to absorbed Ig was discounted it was possible to demonstrate monoclonal Ig labelling in seven out of sixteen cases of non-Hodgkin's lymphoma. In each case IgM was found in association with a single light chain type and these results were in agreement with those obtained by direct immunofluorescent labelling of cryostat sections. In a further case u chains without associated light chains were demonstrated by immunoperoxidase staining. Seven cases of Hodgkin's disease were studied by immunoenzymatic techniques. Although IgG was frequently found in Reed-Sternberg and Hodgkin's cells its presence was not attributable to non-specific uptake of serum protein since albumin was absent or only present in small amounts. These findings are in support of the macrophage origin of these cells.

Fixatives↗
PubMed1979

The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells.

The cellular distribution and the differential expression of HLA on cell suspensions and tissue sections has been investigated using the monoclonal antibody W6-32, which reacts with the high molecular weight chain of the major histocompatibility antigen. Lymphocytes and platelets, as assessed by autoradiographic and immunoperoxidase labeling, were the most densely labeled cells. Myeloid precursors showed more labeling than mature neutrophils. Electron microscopic immunoperoxidase labeling showed a continuous distribution of HLA antigen on lymphoid and myeloid cell membranes. Erythroid precursors (including reticulocytes), although very weakly labeled, were clearly positive, in comparison with mature erythrocytes. In the thymus, HLA-negative, thymocyte antigen-positive cells (85%) can be distinguished from HLA-positive, thymocyte antigen-negative cells (15%). By using immunofluorescence techniques on tissue sections, the former cells were shown to be cortical thymocytes and the latter medullary cells.

Antigens, Surface↗
PubMed1979

Treatment of anaerobic infections with metronidazole.

Metronidazole (Flagl), a compound widely used in man with minimal side-effects, has been used in the treatment of anaerobic infections caused by Bacteroides fragilis and other Bacteroides species. Seven patients were treated and all were restored to full health. Four of them did not respond to lincomycin or clindamycin which so far have been the drugs of choice against anaerobic infections. The reasons for the good results during metronidazole therapy such as the good penetration through the blood/brain barrier and into abscess cavities are discussed.

Adult↗