Biomedical subjects
Publications and source records attributed to B Cylwik.
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The aim of the study including 89 brain gliomas was to determine their proliferative activity assayed with immunohistochemical methods (PCNA and Ki-67) and with the method of AgNORs, as well as to evaluate the correlation between the proliferative activity and features of histological malignancy. The study reveals that the estimation of PCNA, Ki-67 and AgNORs are effective methods for the determination of the proliferative activity of brain gliomas. Statistically significant differences were noted in the proliferative PCNA, Ki-67 and AgNORs between groups of gliomas with lower and higher malignancy, which indicated a distinct correlation between histological malignancy of the tumours and their proliferative activity. High values of PCNA and Ki-67 (> 40%) and AgNORs (> 15) were found to considerably deteriorate prognosis in brain gliomas.
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The effect of asparagine and arginine on the repair of isoprenaline-damaged myocardium was studied. In group I (treated with isoprenaline) the rats developed large foci of myocardial damage filled by connective tissue after 14 days. Similar changes were observed in group II (isoprenaline + asparagine). In group III of rats treated with isoprenaline and arginine the number of foci of injury and their size were smaller, and only a few small foci fibrosis were found after 14 days. Arginine seems to protect the rat myocardium from isoprenaline-induced damage. The repair by connective tissue is supplemented by compensatory hypertrophy of muscle fibers.
The effect of cytotoxic drugs was tested on the hematopoietic system by assay of the ability of granulocyte-macrophage colony forming cell (GM-CFC) in mice to create GM colonies. The in vivo ability of bone marrow progenitor cells to granulocyte-macrophage colony formation, was tested after long-term peritoneal cytotoxic drug administration. The direct effect of these agents on cells in vitro culture was evaluated also. It was found that cytotoxic drugs inhibit the GM colony formation. The degree of damage to the bone marrow progenitor cells by assaying in vivo colony formation inhibition, depends on the drug dosage and length of therapy (after 25-30 days of treatment the colony growth was below 50%). The in vitro inhibition of granulocyte-macrophage colony formation depends on the concentration of drug (10(-7)-10(-5) M is critical for GM colony growth). The results suggest the possibility of the GM-CFC growth testing as an indicator of the progress or side effects of cytotoxic therapy.