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B D Cookson

Publications and source records attributed to B D Cookson.

66 records · Page 4Linked to original sources
PubMed1989

Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus.

A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial patterns were used as the basis of a computer-assisted numerical analysis. The MRSA strains clustered into four phenons at the 83% similarity level; and further division of phenon 1, at the 86% similarity level, resulted in a total of six clusters. All of the MRSA isolates from an MRSA epidemic in the United Kingdom were found to cluster in phenon 1 together with 9 of the 12 MRSA isolates from eastern Australia and 3 other MRSA isolates from the United Kingdom. The remaining three eastern Australian isolates clustered separately in phenon 2. Phenon 3 appeared to be exclusive to strains that were both susceptible and resistant to methicillin and that reacted with group V phages, and phenon 4 comprised 11 isolates, all of which were other MRSA isolates from the United Kingdom. We conclude that computer-assisted numerical analysis by high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins provides additional criteria for the study of the epidemiology and the evolution of MRSA.

Bacterial Proteins↗
PubMed1988

'Cialit' as a tissue preservative: a microbiological assessment.

We describe bacterial contamination of a 'Cialit'-preserved cartilage bank which continued after a variety of changes to the harvesting and preservation protocols during a 3-year prospective study. Our results emphasize the importance of adequate tissue bank microbiological screening. Alternative methods of tissue preservation should be considered.

Bacteria↗
PubMed1988

Epidemic methicillin-resistant Staphylococcus aureus.

We contrast the experiences, in our Health Authority in South-East London, with the particular epidemic methicillin-resistant Staphylococcus aureus (the EMRSA) strain that has recently spread widely around London and South-East England, and with the other MRSA (OMRSA) strains encountered there. Our isolates of the EMRSA were identical by chromosomal restriction enzyme analysis, and the chromosomal and plasmid phenotypes were similar to those described in North London and Eastern Australia. Experimental phage-typing distinguished them from OMRSA encountered in 1984 to 1986. Between 1984 and 1985, the EMRSA caused increased infection and patient colonization compared to the years 1969 to 1983. A change in infection control procedures was usually required to control the EMRSA and in 1986 isolates had returned to their pre-1984 levels. Between 1984 and 1986 OMRSA were still encountered, but did not spread or require changes in infection control procedures. The distribution of other resistant isolates was examined; c 94% of neomycin-resistant isolates were in-patients or clinic patients. Forty-five different phage-type/antibiogram patterns were found in 88 isolates from 66 patients between 1982 and 1985, and patient clusters were uncommon. The ability of the EMRSA to spread is discussed and is probably not purely organism related. Our experience supports the contention that some MRSA are truly epidemic, whilst others do not behave in this manner.

Humans↗
PubMed1985

Mycobacterium szulgai--a case of cutaneous infection?

An infant presented with a carbuncle over the angle of her jaw which grew a scotochromogenic mycobacterium, subsequently identified as Mycobacterium szulgai. The problems of identification of this organism and its possible pathogenic role are outlined.

Chromatography, Thin Layer↗
PubMed1984

The use of a biotyping system to investigate an unusual clustering bacteraemias caused by Aeromonas species.

Aeromonas spp. were isolated from blood cultures taken from four clinically bacteraemic patients over an 18 day period on four separate wards. A common source was suspected and extensive environmental sampling revealed two more ward isolates of Aeromonas spp. A biotyping system was employed which distinguished the strains from each other and indicated that a common source was unlikely. This coincidental clustering occurred in the autumn, a period when isolates from water and faeces are normally at a peak. All patients were debilitated and it is postulated that their own gastrointestinal tracts acted as the most likely route for their bacteraemias.

Aeromonas↗