PubMed Health⌕ Search

Biomedical subjects

B Danic

Publications and source records attributed to B Danic.

At least 19 recordsLinked to original sources
PubMed2005

[Incidents of blood donation].

In France, more than two million blood donations are needed for transfusion each year. The rules and requirements for blood donors selection are strict and aim to protect the donors' health as well as the patients'security. However, the review of the literature shows that adverse effects of blood withdrawal occur quite frequently. They can be classified in immediate or delayed events, or local (hematoma, nerve injury, arterial puncture injury, allergy, veinitis, thrombosis) and general reactions (vasovagal reaction, citrate toxicity, cardiovascular failure, allergy to ethylene oxide, adverse effects of drugs used for some types of donation). This article reviews the principal adverse events described in the literature and concludes with the necessity to set up hemovigilance for donors, as defined in directive 2002/98/EC of the European parliament and of the council.

Adult↗
PubMed2005

[Stating technical requirements and deferral criteria for whole blood donation].

Blood donation is framed by regulations whose finality is to guarantee the safety of blood transfusions and to preserve the health of the donors. Each blood donation is preceded by a medical interview which evaluates the aptitude of the donor to support a 400 to 600 mL collection of blood or its components, and checks the absence of exposure to blood-borne diseases. The criteria of exclusion of blood donation are defined on clinical and epidemiologic arguments. The aim of blood donors selection is consisting in managing the residual infectious risk caused by known pathogens, risk related to the window period due to recent contaminations, the absence of technique of pathogens inactivation available for the cellular blood products, and the risk inherent in any production process. Blood donors selection proved to be the only means of reducing the risk of transmission of an emergent agent by blood transfusion.

Blood Donors↗
PubMed2004

Alkaline single-cell gel electrophoresis (comet assay): a simple technique to show genomic instability in sporadic breast cancer.

The alkaline comet assay was used to study the genomic instability of lymphocytes derived from untreated sporadic breast cancer patients (50 cases), and also following their in vitro irradiation up to 5 Gy. We compared the results (mean tail moment (MTM)) with a control population of 25 patients and with breast cancer patients who had been 'cured' of their disease, with a follow-up of 10 years or more (25 cases). At the basal level, 77.5% (P<0.01) of the untreated patients and 73.7% (P<0.05) of the 'cured' women had values higher than the basal cut-off level of 5.3, compared with only 44% of the controls. After in vitro irradiation, 83% of the untreated patients were above the cut-off value of 10.8 at the 5-Gy dose compared with only 48% of the controls (P<0.01). These results support the hypothesis that women affected by sporadic breast cancer have a constitutional genomic instability. The assessment of the prognostic value of this test could be of interest, particularly in women without axillary nodal involvement.

Aged↗
PubMed2003

[Introduction].

Explore the source record for details and available documents.

Blood Donors↗
PubMed2003

[How human sciences can help to understand blood donation?].

In France, the necessity of managing sanitary risks associated with blood transfusion has induced a strong medicalization of blood collection. Practicing this activity of transfusion medicine confronts the professional with problems which the medical science is not sufficient to answer: curbs and motivations for donating, seasonal and geographic fluctuations in blood collection, sense of pre-donation interview, individual or collective contestings of guidelines for blood collection. From recent sociological works on donation in France, especially on blood donation, this paper suggests reporting the complexity of blood donation thought process and the necessity to refer to human and social sciences to approach it in a best way. Understanding the gift should help professionals to communicate not only on the promotion of blood donation, but also on the risk and its management.

Blood Component Removal↗
PubMed2003

[Clinical selection of blood donors].

For 20 years, the organization set up to insure the blood transfusion safety has never stopped strengthening. It is based on clinical and epidemiological selection of the blood donation candidates, biologic selection of blood donations and different physico-chemical techniques for pathogens reduction or inactivation in blood products. In France, this organization is optimized by the assertion of the voluntary and non-remunerated character of blood donation registered in the law of January 4th, 1993. The blood donors selection is structured in three successive stages. The first stage is the pre-donation information. The second stage begins with reading and filling out an info-questionnaire which prepare for an interview with a physician. This interview is specially directed to prevention of transfusion-transmitted infections and the prevention of adverse reactions after a 400 to 600 mL collection of whole blood or components. Finally, the third stage is the delivery of a post-donation information which invites the donor to contact the "établissement français du sang" (EFS) in case of a new event arisen after the donation or in case of reviewing of its own answers during the medical interview.

Blood Donors↗
PubMed2002

[Creation and organization of a mobile collection unit].

In France, nearly eighty per cent homologous blood donations are given in mobile settings. The collection site requirements, and particularly premises, are conditioning the security of persons, blood products quality, and blood collection efficiency. They also take a decisive part in the public image of the transfusion network. This paper describes an easy method for evaluating and validating premises used for mobile setting of blood collection.

Blood Donors↗
PubMed2002

[Is it possible to organize into a hierarchy blood donation contraindications?].

Because it symbolizes henceforth the sanitary risk and fears which are linked to it, the blood transfusion must master the potential or real risks associated to its practice. The analysis of these risks leads to confirm the initial selection phase of candidates for blood donation as an always original stage of the blood transfusion safety towards the identified infectious risks, but also mainly towards the emergent or modelled risks. The evaluation of the current system of prevention leads to consider two potential dangers: the ineffectiveness of the selection because of the lack of meaning given to this stage, and the donors' disaffection caused by badly accepted or badly justified deferrals. The deficit of meaning can be due to an insufficient information of the population as for the policy of collective prevention of transfusion-transmitted infectious diseases. It can be worsened by the construction of the pre-donation interview which can appear as a succession of questions without visible links. This paper suggests risk analysis to blood transfusion, and a reflection to improve this important stage of blood product safety approach represent by the selection of candidates to a blood donation.

Blood Donors↗
PubMed2001

[Clinical and biological qualifications for blood donations].

Three major steps are very important to increase transfusion safety: epidemiological step: donor selection; biological step: qualification of each blood donation; technological step: obtention of final product. The final therapeutical blood products must meet the requirements concerning their quality and safety, two factors with which the population is very demanding. These requirements can be ensured only if ethical, clinical epidemiological and biological parameters are clearly defined. The strict application of these criteria to each step of the transfusion process, from the blood donation to the final product, allows us to ensure the security of recipients with regard to known risks but also to anticipate other emergent risks.

Blood Donors↗
PubMed2000

[Discovery of a chronic HVC infection without seroconversion in a blood donor in France during 28 months].

The HCV-RNA screening technique developed by the French Fractionation and Biotechnology Laboratory singled out in March 1998 a case of positive HCV-RNA viremia in a blood donor without any anti-HCV antibody. That donor was a 46-year-old woman who had made 54 donations of blood products from 1988 to 1997. She had no history of blood transfusion, no history of hepatitis and no life-style risk factor. Clinical examination was normal. Liver tests (serum alanine amino transferases, gamma glutamyl transpeptidase , alkaline phosphatase, bilirubin , prothrombin and albumin) were normal. Total blood count was normal. Lymphocyte count was normal as well as in vitro functional analysis of lymphocytes (stimulation with different antigens). All screening HCV Elisa tests and immunoblot System available on the French market were unable to detect anti-HCV antibodies. Quantification of serum HCV-RNA (Amplicor Monitor Roche) showed 294,000 copies/mL and HCV genotype 1b determination was performed using Innolipa assay. Further examination of the HCV genotype by direct sequencing of the PCR product showed a classical 1b genotype sequence. The hemovigilance inquiry identified 25 labile products distributed since 1988. Analyzing the records of the recipients that have so far been traced and identified revealed three periods: 1997 to 1995: three recipients were found to be positive for anti-HCV antibodies; two are now cured of hepatitis C. In one recipient, direct sequencing after specific PCR of the hypervariable region coding for the envelope domain showed 100% homology with the donor; 1993 to 1990: four recipients were identified and traced without contamination; in 1988: three of four blood product recipients were anti-HCV negative without HCV-RNA viremia. The forth carried anti-HCV antibodies and genotype 1b HCV-RNA but had a history of multiple surgery. Alter et al. [4] and Bush et al. [5] have previously suggested the possibility of a chronic, immunologically silent state of infection. The case described herein, is the first evidence for this hypothesis. Indeed, the donor has not yet seroconverted 28 months after viremia was discovered. This blood donor was identified by HCV-RNA screening of plasma products. The identification of the same sequence in a recipient of blood from this donor clearly establishes the transmission of the virus by transfusion. The prevalence of such cases of infectious silent chronic HCV carriers has to be determined and the mechanisms responsible for the absence of antibody production need to be clarified.

Amino Acid Sequence↗
PubMed2000

[The physician's role in blood donation].

In France, proper transfusion practices impose that the medical pre-donation interview be done by a physician. In Anglo-Saxon countries, it is more often performed by a nurse. The physician's job is not limited to the clinical selection of blood donation volunteers. In the production process, the physician is implicated in the safety and supply of labile blood products. The practice of the profession requires educational and communication skills. As the manager of a medical team working in mobile sites, the physician must ensure the respect of ethics and professional regulations by the staff he or she trains and supervises.

Blood Banks↗
PubMed2000

[Quality indicators and dashboards: the experience of a collection department].

Production of labile blood products and pharmaceutical products derived from blood must meet quality and safety requirements set and controlled by regulatory provisions (Guidelines for Transfusion Practices, Characteristics of Labile Blood Products). With its activities exerted at the start of the transfusional chain, collection departments have to meet internal requirements (self-sufficiency, procurement regulation, compliance of blood products as raw materials) as well as external ones (donor and recipient safety). To fit in a dynamics of on-going quality improvement, the quality approach of a department must implement a system to monitor and control its activities. The subject of this work is to report an experience of the implementation of quality indicators and specific dash boards for whole blood collection and related activities in a blood transfusion center.

Blood Banks↗