Biomedical subjects

B Donnelly

Publications and source records attributed to B Donnelly.

22 records · Page 2Linked to original sources

PubMedDate not supplied

Determination of basic drugs in post mortem tissues: a microextraction technique utilizing GLC/NPD of effluents.

A method is described for the estimation of basic drugs and metabolites in post mortem tissues and fluids. The method is based upon the selective extraction of these substances, differential weak acid cleanup, and the final preparation of a microextract that is compatible with the rubidium source found in most nitrogen-phosphorous detectors (NPD). Application of this extract to gas chromatography/NPD allows for the measurement of common basic drugs at subtherapeutic, therapeutic, and toxic concentrations in a variety of tissue matrices (liver, brain, lung, heart, and kidney) using a minimum of sample (less than one gram). Internal standards are used to allow for the quantitative as well as qualitative aspects of the assay. Examples of tissue extract chromatograms containing commonly encountered basic drugs are presented along with precision data.

Cadaver↗

PubMedDate not supplied

Detection and quantitation of multiple volatile compounds in tissues by GC and GC/MS.

Qualitative and quantitative analysis of tissues and body fluids for multiple volatile organic compounds were performed by a combination of packed and open tubular capillary GC and GC/MS. This paper describes methods for such analyses in a case involving the exposure of two persons to methyl ethyl ketone, methyl isobutyl ketone, toluene and the three isomeric xylenes. Tissue and body fluid concentrations of these substances in the two decedents are presented and discussed briefly.

Butanones↗

PubMedDate not supplied

Determination of succinylcholine in tissues by TLC, GC/NPD, and GC/MS.

Succinylcholine, a bis-quaternary ammonium compound, is considered an elusive analyte due to rapid hydrolysis by pseudocholinesterase in plasma. However, tissue acetylcholinesterase is relatively inactive toward this substrate. Hence, analysis of tissues of succinylcholine-treated animals may reveal the presence of unchanged drug. This report describes three chromatographic techniques (TLC, GC/NPD, and GC/MS) which may be applied to the analysis of this substance in human tissues. Validation of the techniques was accomplished using tissues from rats treated with succinylcholine.

Animals↗

PubMedDate not supplied

Evaluation of the methadone-alcohol interaction. I. Alterations of plasma concentration kinetics.

Clinical anecdotal reports have indicated a probably additive and/or synergistic response to the co-ingestion of ethyl alcohol and methadone. This study investigated the possible explanations for this observations, which may be associated with alterations of the plasma concentration dynamics of methadone and alcohol in the rat. Plasma concentrations of ethanol, methadone or both were followed over an eight hour period following substance administration. GC/FID and GC/MS were employed to quantify ethanol and methadone, respectively. The results of this study indicated that ethyl alcohol significantly increased peak methadone concentrations. Further, methadone significantly depressed late alcohol elimination.

Animals↗