Biomedical subjects

B Dudkiewicz

Publications and source records attributed to B Dudkiewicz.

6 recordsLinked to original sources

PubMed2004

Inhibitory effect of furosemide on activation of human peripheral blood polymorphonuclear leukocytes stimulated with n-formyl-methionyl-leucyl-phenylalanine.

The clinical efficacy of inhalatory furosemide (Fu) has been extensively studied in bronchial asthma patients but there are only a few studies addressing its action on cells participating in the underlying inflammatory process. Therefore, we investigated the effect of Fu on human peripheral blood polymorphonuclear leukocytes (PMNL) at concentrations that can be achieved in the bronchial lining fluid by inhalation, i.e. 10(-5), 10(-4) and 10(-3) M. The influence of Fu on the following PMNL parameters was investigated: intracellular calcium changes ([Ca2+]i) as a part of signal transduction and luminol dependent chemiluminescence (LCL) as an indirect measure of NADPH-oxidase activation upon n-formyl-methionyl-leucyl-phenylalanine (fMLP) stimulation; chemotaxis to fMLP, phagocytosis and intracellular killing of Staphylococcus aureus. Incubation with Fu resulted in a concentration dependent reduction of Ca2+ influx and Fu (10(-3) M) decreased the main Ca2+ parameters to one half of the control values and to the level obtained in calcium-free buffer. In contrast, Fu had no effect if preincubated with the cells and then removed by washing. The LCL signal was reduced by Fu (10(-3) M) from 2000 +/- 870 to 550 +/- 440 arbitrary units [aU] (p<0.05). In contrast to the [Ca2+]i measurements, a slightly diminished LCL was also observed following preincubation with Fu and washing. No effect of Fu was found on phagocytosis and intracellular killing of St. aureus. Fu diminished chemotaxis to fMLP but at 10(-3) M it also displayed weak chemoattractant properties. The differential action of Fu on human PMNL may add to the understanding of its topical and restricted efficacy in bronchial asthma.

Calcium↗

PubMed2001

[Alive microorganism in the workplace ambient air in plants disposing communal waste].

This paper presents the results of the identification and determination of alive microorganisms in the air samples collected from the breath zone of workers employed in the waste collection and disposal. Samples were taken during waste collection and in the waste reloading, sorting and composting plants. In addition, samples were taken at stationary sites: in the city and inside flats. Different kinds of species of Gram-positive bacteria (Micrococcus, Staphylococcus, Enterococcus, Bacillus, Listeria, Corynebacterium, Rhodococcus) and Gram-negative rods (Enterobacteriaceae, Pseudomonas and other aerobic bacteria) were found. On every work site, there were fecal bacteria (Escherichia coli, Enterococcus faecalis and Enterococcus faecium). The city areas and flats were free from this kind of bacteria. The number of mesophilic bacteria and thread-like fungi exceeding 10(4) cfu/m3 of air was counted on every work site. The largest number of mesophilic bacteria and Actinomycetes were found in the waste composting plant (10(4)-10(6) and 10(3)-10(5) cfu/m3, respectively), whereas the majority of fungal spores were counted during waste collection and sorting. (10(4)-10(5) cfu/m3). On the city streets there were no Actinomycetes and the number of fungal spores (10(4)/m3 of air) was similar to that observed in the waste reloading and composting plants. The amount of mesophilic bacteria in the city and flat samples was below 10(2)-10(3) cfu/m3. As to the number of fungal spores and Actinomycetes there was a limit of determination (10(1)-10(2) cfu/m3). The majority of mesopholic bacteria identified in the air were classified as the second group of occupational risk in the Council Directive 54/2000/EU. Bearing in mind that Gram-negative rods (especially intestinal) were identified in all samples, it is suggested to use endotoxin concentration as one of possible criteria for assessing the hygiene conditions at workplaces.

Air Microbiology↗

PubMed2001

[Assessment of exposure to bioaerosols in workplace ambient air during municipal waste collection and disposal].

The assessment of the exposure to bioaerosols among workers engaged in the collection and disposal of municipal waste is presented. The workers were divided into the following groups, depending on the job performed: waste collectors (loaders, drivers), composting plant workers (heavy equipment operators, waste site workers), sorters (heavy equipment operators, waste sorters) and landfill site workers (heavy equipment operators, weighers). Air samples were also collected on the city streets and in flats. They were reference points to the results obtained. Air samples were collected at the breathing zone. The concentrations of dust, viable microorganisms (mesophilic and thermophilic bacteria, and fungi), endotoxins and the total number of microbiol cells were determined. The highest, individual exposure to dust was found in composting plant workers and waste collectors. The values exceeded considerably maximum allowable concentrations (by ten times at composting plant). The mean values for the above listed groups also exceeded the concentration of 4 mg/m3. Dust concentrations at other workposts were substantially lower. The concentrations in the city streets and in flats maintained at the level of 0.1 mg/m3. Endotoxin concentration at a protecting upper airway inflammation level (10 ng/m3) was exceeded in the majority (above 50%) of samples. That is why the average concentration in individual groups was also higher than 10 ng/m3. Street and flat samples included endotoxins at the level of 1 ng/m3. Taking as a criterion the obligatory total dust and endotoxin MAC value of 10 ng/m3 for assessing work hygiene conditions, it should be stated that waste collectors and composting plant workers were employed in conditions of poor hygiene.

Adult↗

PubMed1996

[Coagulase-negative staphylococci isolated from cases of infective endocarditis].

In this study we investigated the properties of 47 strains of coagulase-negative staphylococci (CNS) isolated from patients with infective endocarditis (IE) and 61 strains of CNS isolated from the skin flora of healthy people. Significant differences between the properties of CNS from IE and from the skin flora were seen in the production of protease, lipase, fibrinolysin, siderophore and the ability of hydrophobic CNS strains to adhere to buccal epithelial cells. CNS from IE and from the skin flora did not differ in the production of glycocalyx, capsule, hemolysin and fibronectin and collagen binding. Multiple resistant strains were present among CNS isolated from patients with IE.

Cheek↗

PubMed1993

[Etiology of bacterial endocarditis in materials from Cardiology and Cardiac Surgery Clinics of the Lodz Academy].

In the years 1986-1991, 72 cases of bacterial endocarditis (BE) were investigated. The bacteria were isolated from blood in 24 cases, from native and artificial valves obtained during surgery in 24 cases, and from both blood and valves in 6 cases. Following microorganisms were isolated: S. epidermidis, S. xylosus, S. hominis, S. aureus, Micrococcus sp., S. mitis, S. sanguis, S. faecalis, M. lacunata, E. adecarboxylata, P. maltophila, Corynebacterium sp., A. calcoaceticus var. lwoffii, and Aspergillus sp. Gram-positive bacteria were prevailing among isolated strains. Staphylococci were present in 55.7% (29 cases) and among them coagulase-negative staphylococci occurred in 89.7% (26 cases). Streptococci were isolated from 9 patients (17.3%) and Gram-negative rods from 5 patients (9.65%). Coryneform bacteria were detected in 7 cases (13.5%). There was no relation between type of removed valve and colonizing it bacteria. From native and artificial valves coagulase-negative staphylococci were isolated, respectively, in 48.3% and 52.2%. Susceptibility of isolated bacteria to 27 antibiotics was determined. Multiple resistant strains were present most frequently among staphylococci.

Actinomycetales↗

PubMedDate not supplied

Infective endocarditis--changes in the microbiological profile.

The clinical material from 120 patients, with clinical diagnosis of infectious endocarditis (IE) (65 patients with IE on native valves and 55 patients with IE on artificial valves, i.e. mechanical or biological prosthesis) treated during the period of 1986-1994 was investigated. Sixty positive cultures were obtained from the valves and 40 positive cultures from the blood, which accounted for 76% and 60% of the confirmed IE cases, respectively. Bacteriological confirmation of IE was obtained in 91 (75.8%) patients, 80.2% of the isolated microorganisms being gram-positive bacteria, 17.6% gram-negative, and 2.2%-fungi. The most frequently isolated bacteriawere coagulase-negative staphylococci. They accounted for 40.5% of infections in the IE patients on native valves, and for 63.3% in those with artificial valves. Streptococci were isolated from 11.8% of the patients with IE on native valves and from only 2% of cases of IE on artificial valves. The multiresistant strains were most frequently isolated from the patients with IE on artificial valves.

Endocarditis, Bacterial↗