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Biomedical subjects

B Dunlap

Publications and source records attributed to B Dunlap.

26 records · Page 2Linked to original sources
PubMed1980

Family of large surface proteins on human lymphocytes.

Cell surface molecules of T-rosette-positive human peripheral blood lymphocytes were labelled by lactoperoxidase and examined on 5% polyacrylamide gels. Several bands, ranging in molecular weight from 220,000 to 170,000 daltons, were seen. Analysis of the tryptic and chymotryptic digests of the 187K, 200K, 210K and 220K proteins indicated that they are of similar peptide composition. Study of clones derived from this population indicated that individual cells within a population differed in expression of these proteins.

Animals↗
PubMed1980

Molecular relationships between large membrane proteins (LMP) expressed on T and B lymphocytes.

Clones prepared from day 5 mixed lymphocyte cultures (MLC) were examined for the expression of large (170,000- to 200,000-dalton) membrane proteins (LMP), found on bulk cultures of resting and allogeneically activated T lymphocytes. Sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE) of these proteins indicates both bulk populations and noncytotoxic clones express LMP of similar m.w. Peptide mapping further indicates that LMP of 187,000 (187K) and 200K daltons, found on T cells from bulk cultures or clones and the 220K dalton LMP from B cells, all appear to have a very similar peptide composition. This suggests a single protein (or series of closely related proteins) is differentially processed in functionally disparate populations, and hence may serve as a differentiation antigen for these populations.

Animals↗
PubMed1978

Activation of neutrophilic granulocytes by products of human lymphocytes.

Materials produced by stimulated primary cultures of human lymphocytes and by a continuous human lymphoid cell line, RPMI 1788, were tested for granulocyte phagocytosis promoting activity. Blood lymphocytes of healthy donors were stimulated with tuberculin (PPD) or concanavalin A (con A) and the culture fluids collected after 3 days of in vitro incubation. Fluids of the lymphoid cell line were collected from 2-day-old cultures. All three kinds of preparations were found to induce a significant increase in neutrophilic granulocyte phagocytic activity. Studies on the kinetics of granulocyte activation revealed that it is a relatively rapid process, being maximal within 60-120 min. This is in contrast to the considerably slower activation of monocytes in a comparable system. Intracutaneous inoculation of the active materials in human volunteers resulted in reactions that grossly and histologically resembled accelerated delayed type hypersensitivity reactions.

Granulocytes↗
PubMed1977

A T-cell marker in mouse fibroblast x T-lymphocyte somatic cell hybrids.

Continuous hybrid cell lines have been generated by the fusion of allogeneically primed murine T lymphocytes with mouse L-cell-derived fibroblasts of the line 613. The resulting hybrid clones express one or more T-cell-specific surface components identifiable by radioiodination of intact cells followed by NP-40 solubilization of membrane proteins and sodium dodecyl sulfate polyacrylamide gel electrophoresis. These markers are stably expressed by the hybrid lines after several months of passage.

Animals↗
PubMed1977

Enhancement of granulocyte phagocytosis by products of PPD stimulated human lymphocytes.

The effect of supernatant fluids from cultures of stimulated human lymphocytes on the phagocytic activity of human neutrophilic granulocytes was investigated. It was found that lymphocytes of tuberculin (PPD) positive donors after in vitro stimulation with PPD produced factors that significantly increased granulocyte phagocytosis of latex particles.

Granulocytes↗