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B E Damberg

Publications and source records attributed to B E Damberg.

7 recordsLinked to original sources
PubMedDate not supplied

[Effect of protein synthesis inhibitors on the activity of mitochondrial enzymes of Saccharomyces cerevisiae during desiccation].

The work deals with the effect of carbon sources, presence of protein synthesis inhibitors (cycloheximide and chloramphenicol) and dehydration regime on the enzyme activity of the dried yeast Saccharomyces cerevisiae. The yeast grown on molasses and dried by aeration demonstrated a notable increase of the NADH-dehydrogenase and succinate dehydrogenase activities as compared with the analogously treated yeast grown on ethanol. The latter showed a notable rise only in the activity of NADH-dehydrogenase during slow drying. Addition of protein synthesis inhibitors into the cultivation medium caused no decrease of activities of the above mentioned enzymes of the dried yeasts in any variant under study.

Desiccation↗
PubMedDate not supplied

[Toxic effect of cysteine on cells of Saccharomyces cerevisiae growing on media of various compositions].

The oxidative processes in Saccharomyces cerevisiae were shown to be more susceptible to the toxic effect of cysteine as compared to the glycolytic processes. When cysteine was added to a suspension of resting cells, the activity of catalase and oxygen uptake decreased, particularly in the yeast was grown in a medium preventing catabolyte repression. As was demonstrated using disc-electrophoresis in acrylamide gel, cysteine decreased the activity of catalases T and A.

Alcohol Oxidoreductases↗
PubMedDate not supplied

[Protein yield in the rehydration of dehydrated Saccharomyces cerevisiae yeast cells].

The release of proteins was studied during rehydration of Saccharomyces cerevisiae cells dehydrated using two techniques which were characterized by different survival rate. A correlation was established between the loss of proteins and the survival rate of cells after dehydration and rehydration. Proteins with a molecular mass from 6000 to 70,000 daltons (low molecular weight proteins predominating) were found in the rehydration medium by the method of disc-electrophoresis in polyacrylamide gel in the presence of sodium dodecylsulfate. Moreover, the rehydration medium exhibited the activity of certain phosphohydrolases and dehydrogenases; this finding suggests that proteins with a higher molecular weight are present in the medium.

Desiccation↗
PubMedDate not supplied

[Effect of cultivation conditions on the electrophoretic spectrum of various dehydrogenases in Saccharomyces cerevisiae].

The activity and electrophoretic spectrum of glucose-6-phosphate dehydrogenase, alcohol dehydrogenase, and malate dehydrogenase in Saccharomyces cerevisiae 14 depended on the conditions of cultivation, i. e. the sources of carbon and their concentration, the content of nitrogen, and aeration of the medium. A heterogeneous tris-buffer protein fraction was isolated after disintegration of the cells; the dehydrogenases were represented by several molecular forms whose number, electrophoretic mobility, and activity depended on the conditions of cultivation.

Aerobiosis↗