PubMed Health⌕ Search

Biomedical subjects

B E Dodd

Publications and source records attributed to B E Dodd.

At least 19 recordsLinked to original sources
PubMed1984

Experience in the detection of red cell antigens and HLA antigens in bloodstains.

The red cell antigens A, B, H, C, CW, c, D, E, e, S, s, K, Fya, Fyb, Jka and Jkb can all be detected on bloodstained threads using a modified Landsteiner elution technique. Bloodstain material is often very limited in quantity and far from fresh so every effort is made to produce optimum conditions throughout the test including extension both of the absorption period and the elution time, using a low concentration of indicator cells and the use of LISS. Detection of the HLA antigens in stains is less advanced but inhibition of the lymphocytotoxicity of HLA sera by bloodstained threads has produced some promising results in experiments involving the HLA-A1, A2, B8 and CW3 antigens.

Blood Group Antigens↗
PubMed1982

Elution of group-specific substance A from RBC of various subgroups of A and its effect on the agglutination of AX RBC.

Experiments are described which demonstrate that when the Landsteiner heat elution technique is used to prepare eluates from group A cells, some A substance as well as anti-A is released into the eluates. The concentration found is greatest from A1 RBC and least from Ax, suggesting a relationship with the number of antigen sites on RBC of the various subgroups. Further experiments have shown that the presence of this A-specific substance in eluates accounts for the well-know phenomenon that anti-A from selected group O sera, when eluted from A1 RBC, will not agglutinate Ax even though the original serum showed an appreciable titre against Ax. The presence of the eluted antigen also suggests an explanation for the behaviour of antibody recovered from the red cells of various subgroups of A towards Ax.

ABO Blood-Group System↗
PubMed1978

The advantage of a build-up anti-globulin technique for the detection of immunoglobulin on the red cells of rabbits infected with trypanosomes. A preliminary report.

A build-up anti-globulin technique has been used to detect immunoglobulin on the red cells of rabbits infected with Trypanosoma brucei brucei S 42 and 427. The method, which is useful for the detection of immunoglobulin on sparsely coated red cells, involves treating the red cells of infected rabbits with antiglobulin followed by alternate globulin and antiglobulin layers until a lattice is built up which allows agglutination to occur. The red cells of five infected rabbits have been tested with anti-rabbit IgG,-IgM and broad spectrum anti-globulin reagents at intervals after infection and following the administration of Berenil, a drug which destroys trypanosomes. The positive anti-globulin reactions which developed following inoculation with trypanosomes were much reduced after giving Berenil. The tests became more strongly positive again in two rabbits after reinfection.

Animals↗
PubMed1978

The use of low ionic strength solution (LISS) in elution experiments and in combination with papain-treated cells for the titration of various antibodies, including eluted antibody.

The investigation presents evidence for the value of suspending red cells in a low ionic strength medium for elution experiments since this has the effect of increasing the uptake of antibodies by the red cells. In addition it has been shown that for the titration of low affinity antibodies, and also of eluted antibodies, the combining capacity of which may be impaired through the elution process, it is an advantage to use a LISS-enzyme (papain) technique.

Antigen-Antibody Reactions↗
PubMed1978

Serological studies of the H activity of Oh red cells with various anti-H reagents.

Several examples of Oh cells have been investigated with various anti-H reagents. After enzyme treatment of the cells with either papain or neuraminidase, very high titres were obtained, particularly with some preparations of Ulex europeus. Fractionation of anti-H Ulex showed that the anti-H component inhibitable by L-fucose required papain treatment of the Oh cells for optimal activity whereas the component not inhibitable by L-fucose agglutinated preferentially Oh cells treated with neuraminidase. The anti-H lectin Cytisus sessifolius which is not inhibitable by L-fucose gave no reaction with papain-treated Oh cells but reacted well when the Oh cells were treated with neuraminidase. Normal O cells were almost equally well agglutinated by the lectins when treated by either of the two enzymes. That the various lectin components activated by enzymes had H specificity was shown by the fact that their activity was inhibited by purified H substance, partially inhibited to L-fucose and 2-fucosyllactose and not at all by purified Lea substance and non-secretor salivas. In spite of the high titres obtained with Oh cells by the enzyme technique, the amount of H present on the red cells, judged by inhibition tests, in comparison with normal group O cells was very small.

ABO Blood-Group System↗