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Biomedical subjects

B E Gordon

Publications and source records attributed to B E Gordon.

27 records · Page 2Linked to original sources
PubMed1991

Differential white blood cell counts as a preliminary screen for severe combined immunodeficient congenic mice.

Severe combined immunodeficient (SCID) mice are becoming increasingly popular as research animals; as a consequence, more efforts to produce congenic strains carrying the scid gene are underway. In an attempt to conserve time and resources in this endeavor, we used peripheral blood differential white blood cell counts as a preliminary screen to eliminate the homozygous (+/+) wild type and heterozygous (scid/+) animals from intercross generations. The results of our investigation confirm that blood smears can be used as a screen at four weeks of age to identify animals having an inversion of the granulocyte:mononuclear cell ratio. Mice not having an inversion of this ratio, i.e., mononuclear cells exceeding 50%, can be eliminated from the colony. This screen permits elimination of a large portion of the intercross generation one month earlier than other methods that rely on detection of serum immunoglobulin. The screen is highly sensitive and specific. We do not propose that this screen be used as a definitive test but as a tool to eliminate the majority of animals that are not homozygous at the scid locus.

Animals↗
PubMed1989

Impotence: its evaluation and treatment.

More men are seeking treatment for impotence today. Recent advances have resulted in a better understanding of the problem, and a wider array of treatment options. The authors review the pathophysiology of the condition and provide an algorithm for evaluating patients with impotence.

Attitude to Health↗