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Biomedical subjects

B E Lane

Publications and source records attributed to B E Lane.

At least 19 recordsLinked to original sources
PubMed1999

Investigations of the pathway of incorporation and function of lamin A in the nuclear lamina.

Cell-free extracts of Xenopus eggs were used to investigate how differing lamin sub-types are incorporated into a lamina and how lamina composition influences DNA replication. Purified recombinant human lamin A (HlaminA) was inoculated into egg extracts, which support in vitro nuclear assembly. The route of incorporation of Hlamin A into the lamina was compared to Xenopus lamin B3 (XlaminB3), the major endogenous lamin sub-type in egg extracts. While Xlamin B3 was incorporated into the lamina directly, HlaminA first accumulated at nucleoplasmic foci, before entering the lamina. When HlaminA was inoculated into extracts depleted of XlaminB3 it entered nuclei efficiently and was incorporated into nucleoplasmic foci. However, in the absence of XlaminB3, HlaminA remained in the foci and did not enter the nuclear envelope. When HlaminA entered the nuclear envelope, it did not influence DNA replication. Nuclei containing HlaminA initiated DNA replication on queue and in addition the spatial distribution of replication centres in these nuclei was identical to controls. Taken together, these results suggest that the incorporation of A-type lamins into the nuclear envelope is dependent upon the presence of B-type lamins. However, the presence of A-type lamins alone is not sufficient to influence the spatial and temporal order of DNA replication.

Animals↗
PubMed1995

Pancreatic vipomas: spectrum of presentation and evolution of diagnostic and therapeutic modalities.

Two patients are reported who presented with symptoms characteristic of a pancreatic vipoma. The necessity to measure more than one plasma VIP level for diagnosis, and the delay between the onset of illness and diagnosis is illustrated by both cases. Evidence suggests that vipomas are still under reported. The evolution of sophisticated diagnostic and therapeutic modalities over the twenty-five years separating both presentations is discussed.

Combined Modality Therapy↗
PubMed1988

The diagnosis and management of splenic artery aneurysms.

The presentation and course of 7 patients with splenic artery aneurysms is reviewed. Three presented with abdominal pain, 2 with collapse and rupture and in 2 it was an incidental finding. Four patients had elective ligation-excision of the aneurysm with splenectomy as had one patient operated on as an emergency, with no operative mortality. The aneurysm size ranged from 20 to 45 mm (mean 30 mm) and histology confirmed atheroma. One patient was managed as a myocardial infarct for 8 hours after admission and a ruptured splenic aneurysm was diagnosed at autopsy, an overall mortality of 14%. A 66-year-old woman in poor general health was managed expectantly and was asymptomatic when lost to follow-up after 2 years.

Adult↗
PubMed1986

Surgery for Crohn's disease in Ireland.

Patients overall had a one in seven chance of having curative surgery in any of the first ten years following diagnosis. Cumulative surgical recurrence rates are similar to those in America. The higher cumulative surgical recurrence rates are similar to those in America. The higher cumulative risk of surgical recurrence in patients with macroscopic disease of the large bowel probably relates to a conservative surgical approach in such patients. There was no operative mortality.

Adolescent↗
PubMed1980

Single dose intra-rectal metronidazole as prophylaxis against wound infection following emergency appendicectomy.

A prospective placebo controlled randomized trial was carried out in 104 patients with a presumptive diagnosis of acute appendicitis. A single dose of 1 g of metronidazole was given intra-rectally 1 h preoperatively. There were 16 wound infections, 15 (out of 50) in the placebo group, 1 (out of 54) in the metronidazole group. This study supports the already accepted value of metronidazole but suggests that a single intra-rectal dose regimen is adequate for prophylaxis.

Adolescent↗