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B Eberlein

Publications and source records attributed to B Eberlein.

6 recordsLinked to original sources
PubMed2002

Evidence for a new low-lying resonance state in 7He.

Low-lying resonance states in 7He(6He+n), formed after fragmentation reactions of a 227 MeV/nucleon 8He beam on a carbon target, have been studied. Coincidences between 6He nuclei and neutrons, corresponding to the one-neutron knockout channel in 8He, were selected. The relative energy spectrum in the 6He+n system shows a structure, which is interpreted as the 7He ( Ipi = 3/2(-)) ground state, unbound with 0.43(2) MeV relative to the 6He+n system and a width of Gamma = 0.15(8) MeV overlapping with an excited ( Ipi = 1/2(-)) state observed at 1.0(1) MeV with a width of Gamma = 0.75(8) MeV.

Journal Article↗
PubMed1992

Viral culture and p24 antigenemia of human immunodeficiency virus (HIV)-infected individuals correlated with antibody profiles determined with recombinant polypeptides of all HIV-1 open-reading frames.

The association between viral activity and antibody profiles was investigated in 202 individuals infected by the human immunodeficiency virus (HIV) grouped according to their Walter Reed clinical stage. Each study group was subdivided into subjects positive or negative for markers of active viral replication: presence of serum p24 antigen and viral culture. In Western blots using recombinant antigens, sera of HIV-positive individuals with positive viral markers had a significantly lower antibody reactivity to several viral proteins than did individuals without viral markers. Noticeably, proteins of the gag (p24, p17) and env (gp120, COOH-terminal part of gp41) open-reading frames revealed a decreased reactivity. The antibody response to the regulatory proteins revealed no or poor association with viral activity in the host. The results suggest that seroreactivity is mainly influenced by factors reflecting the viral activity of an HIV-infected individual, while the clinical stage of the patient is less important. Especially, reductions in antibodies against gp120 and p17 were useful markers associated with increased viral activity.

Adult↗
PubMed1992

Demonstration of olaquindox phototoxicity in vitro.

Olaquindox is an oral anti-bacterial agent used as an additive to animal food. It is known to cause photosensitization in pig breeders, probably due to a photoallergic mechanism. Also, olaquindox was found to be phototoxic in animal experiments. We have evaluated the phototoxic properties of olaquindox in vitro with a photohemolysis test using different light sources. Irradiation with a source rich in ultraviolet A (UVA) and, to a lesser degree, with a lamp emitting mainly visible light induced photohemolysis, which depended on the concentration of olaquindox and the radiation dose. Exposure to radiation rich in UVB caused no olaquindox-dependent hemolysis. The absorption spectrum of olaquindox changed prominently after irradiation, indicating photodegradation of the compound.

Dermatitis, Phototoxic↗
PubMed1991

Expression of human immunodeficiency virus (HIV) in naturally infected peripheral blood mononuclear cells: comparison of a standard co-culture technique with a newly developed microculture method.

Peripheral blood mononuclear cells (PBMCs) from 29 patients infected with human immunodeficiency virus (HIV) were cultured by two different methods. One was the standard co-culture technique, the other a newly developed microculture method. In this assay 10(6) PBMCs were cultivated in 250 microliters medium, no activating agents or allogeneic cells were present. P24 antigen production measured by this method was found in 7 out of 11 PBMC cultures of patients in the Walter Reed (WR) stage 1 or 2, whereas only 4 samples were positive by the co-culture procedure. Cultures from patients in the later stages of the disease (WR 5/6) showed a higher p24 production by the co-culture method than by the microculture assay. It is assumed that rapidly growing HIV strains can be better assessed by the co-culture method which may select for these strains. P24 expression can be more easily obtained by the microculture technique even in cases where slowly replicating strains may be present. In conclusion, results from the microculture procedure described may be a useful supplementation to findings observed by the co-culture method.

Adult↗
PubMed1989

Ultraviolet A inhibits histamine release from human peripheral leukocytes.

Irradiation of human peripheral leukocytes with ultraviolet A (UVA) induced a significant and dose-dependent reduction of anti-IgE or Ca-ionophore-stimulated histamine release without consistent influence upon C5a-induced release reactions. This effect was equally demonstrable in atopics and controls. In the presence of the radical scavenger superoxide dismutase, the UVA-induced inhibition of anti-IgE-induced histamine release was abolished. Under the conditions used, UVB exposure did not result in relevant changes of in vitro histamine release.

Antibodies, Anti-Idiotypic↗