Isolation and identification of trehalase from Pullularia pullulans.
Trehalase has been isolated from Pullularia pullulans. The enzyme, which is specific for trehalose, was purified approximately 800-fold. The optimal pH was found to be 4.0 and the Michaelis dissociation constant, K(m), was determined to be 3.2 x 10(-3)m.
Carbohydrate Metabolism↗