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Biomedical subjects

B F Miller

Publications and source records attributed to B F Miller.

At least 37 records · Page 2Linked to original sources
PubMed1975

Destruction of Salmonella on poultry meat with lysozyme, EDTA, x-ray, microwave and chlorine.

Lysozyme, ethylenediaminetetracetic acid, chlorine, x-irradiation and microwaves were used in experimental attempts to eliminate Salmonella senftenberg 775W or Salmonella typhimurium from turkey drumsticks and whole carcasses. Turkey drumsticks or whole carcasses were artificially contaminated with S. senftenberg 775W or S. typhimurium in concentrations ranging between 5 X 10(5) to 8 X 10(5) viable cells per ml. of contaminating fluid. After each treatment, samples were cultured, plated, and tested according to standard methods to determine the susceptibility of Salmonella organisms to the particular treatment. A 0.1 percent solution of lysozyme eliminated the S. senftenberg 775W at 22 degrees C. within three hours. A 0.5 percent solution of ethlenediaminetetracetic acid failed to destroy the test organism under the same conditions. Eighty thousand rads of X-ray eliminated the test organism on turkey drumsticks but failed to remove it from whole turkey carcasses. Microwaves eliminated the S. senftenberg 775W in 150 seconds from turkey drumsticks and ten minutes from broiler chicken carcasses. Aqueous solutions containing 3400 and 2125 p.p.m. chlorine failed to destroy the test organism on turkey drumsticks at 21 degrees C. in 9 and 24 hours. None of the treatments changed the appearance of the skin or meat, except microwaves produced a partially-cooked appearance. Chlorine produced off-color drumsticks.

Animals↗
PubMed1973

Subcutaneous bacteria in turkey carcasses.

Two methods were employed to quantitate the subcutaneous bacteria in fresh, refrigerated, and frozen turkey carcasses. Relatively few bacteria were detected in the skin-flesh interface and in the flesh as compared with the number of bacteria on the skin surface and in the skin layer. No subcutaneous bacteria were detected in 49% of the skin-flesh interface and flesh samples. The number of bacteria detected in skin samples from carcasses chemically disinfected to kill skin surface bacteria was smaller than that in nondisinfected skin samples. These results indicate that the skin blending method used to quantify microorganisms on poultry carcass skin measures the skin layer flora and that the number of subcutaneous membrane or flesh bacteria measured is not normally large enough to have a significant influence on the results.

Animals↗
PubMed1970

Optimum skin blending method for quantifying poultry carcass bacteria.

Optimum blending fluids and blending times for use in quantifying bacteria on poultry carcass skin by the skin "blending" method were determined. Butterfield's buffered-phosphate diluent, physiological saline solution (0.85% NaCl), peptone water (0.1% peptone), and deionized water, each at four different skin blending times of 1, 2, 3, and 4 min, were compared. The comparison was based on relative numbers of bacteria per cm(2) of skin, enumerated by each combination on turkey carcasses. Peptone water and physiological saline solution each yielded significantly (P < 0.01) higher bacteria counts from turkey carcass skin samples than did Butterfield's buffered-phosphate diluent or deionized water. There were no significant differences among the four skin blending times and no significant interaction effect between the two factors tested.

Analysis of Variance↗
PubMed1970

Lipase activity in the human aorta.

The hydrolysis of triglycerides by grossly normal male human aortas has been studied in vitro. The tissue contains an acid lipase (pH optimum, 5.4) and an alkaline lipase (pH optimum, 8.8). Both lipases catalyze the hydrolysis of saturated triglycerides; the rate decreases with increasing fatty acyl chain from C(10) to C(18). Glycerol trioleate, trilinoleate, and trilinolenate are hydrolyzed at similar rates. Alkaline lipase is inhibited about 50% at 7.2 mm glycerol trioleate, while acid lipase is unaffected at this concentration. Both lipases are activated by Ca(++) ions. The acid lipase is easily inactivated by deionized water used either as a homogenizing or dialyzing medium. Acid lipase is strongly inhibited by BSA, sodium deoxycholate, and sodium taurocholate; alkaline lipase is unaffected by BSA and is activated about twofold by bile salts. The products of hydrolysis of glycerol trioleate by aortic lipases are predominantly oleic acid and glycerol 1,2-dioleate with a small accumulation of glycerol monooleate. The aortic preparations appear to contain inhibitors for both the acid and alkaline lipase. The substance which inhibits alkaline lipase also inhibits pancreatic lipase; it is heat-stable and dialyzable. The inhibitor of the acid lipase is also heat-stable but is nondialyzable.

Adolescent↗