PubMed Health⌕ Search

Biomedical subjects

B F Smith

Publications and source records attributed to B F Smith.

10 recordsLinked to original sources
PubMed1992

Inherited phosphofructokinase deficiency in an American cocker spaniel.

A 3-year-old female American Cocker Spaniel with a chronic hemolytic disorder and hemolytic crises was found to have M-type phosphofructokinase deficiency. This inherited erythroenzymopathy and myopathy is commonly diagnosed in English Springer Spaniels, but the family study of this Cocker Spaniel, although supporting an autosomal recessive mode of inheritance, did not reveal any English Springer Spaniel ancestors. Molecular genetic studies did, however, identify the same mutation in this dog as we previously reported in the English Springer Spaniel breed, suggesting that this mutation originated prior to the separation of these 2 breeds.

Anemia, Hemolytic↗
PubMed1992

Nutritional outcome and pneumonia in critical care patients randomized to gastric versus jejunal tube feedings. The Critical Care Research Team.

OBJECTIVE: To compare nutritional status, gastric colonization, and rates of nosocomial pneumonia in ICU patients randomized to gastric tube feeding vs. patients fed by an endoscopically placed jejunal tube. DESIGN: Randomized, prospective study. SETTING: Medical and surgical ICUs at Boston City Hospital; surgical ICU at University Hospital. PATIENTS: Of the 38 study patients, 19 were randomized to gastric tube feeding and 19 were randomized to an endoscopically placed jejunal tube. The two groups were similar in age, sex, race, underlying disease, and type of surgery. RESULTS: The two patient groups were similar in number of days fed, duration of ICU stay, duration of mechanical ventilation, days of antibiotic therapy, and days with fever. Compared with the gastric group, the jejunal group had more patients with circulatory shock on admission (79% vs. 68.4%), higher admission Acute Physiology Score (24.0 vs. 21.7), and fewer patients with pneumonia at randomization (26.3% vs. 31.6%). The jejunal group received a significantly higher percentage of their daily goal caloric intake (p = .05), and had greater increases in serum prealbumin concentrations (p < .05) than the patients with gastric tube feeding. Although the jejunal tube group had more days of diarrhea (3.3 +/- 6.6 vs. 1.8 +/- 2.9), this difference was not statistically significant. Nosocomial pneumonia was diagnosed clinically in two (10.5%) patients in the gastric tube group and in no patients in the jejunal tube group. CONCLUSIONS: Patients fed by jejunal tube received a significantly higher proportion of their daily goal caloric intake, had a significantly greater increase in serum prealbumin concentrations, and had a lower rate of pneumonia than patients fed by continuous gastric tube feeding.

Adult↗
PubMed1991

Non-mucin proteins in the matrix of human cholesterol gallstones.

Human cholesterol gallstones contain a pigmented organic matrix that may originate from biliary sludge. The cholesterol gallstone matrix contains mucin, bile pigments, and calcium salts. The goal of this study was to examine whether non-mucin proteins are present in the matrix of cholesterol gallstones. Matrix was prepared from cholesterol gallstones from 18 patients. Proteins were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and by molecular sieve high-performance liquid chromatography (HPLC). Two proteins were present in each gallstone and migrated with or just adjacent to standards of bovine serum albumin on SDS-PAGE. Several additional lower molecular weight proteins were identified, but not in every gallstone. Protein fractions contained visible pigment after chloroform extraction, and pigment co-eluted with proteins on HPLC, suggesting binding of pigments to proteins in the matrix. We conclude that low molecular weight proteins are present in the cholesterol gallstone matrix. The major protein appears to be serum albumin, although definitive identification has not been established. The origin of these matrix proteins and their possible significance in the pathogenesis of cholesterol cholelithiasis is unknown.

Bilirubin↗
PubMed1979

Scanning electron microscopy of the infective eggs of Hydatigera taeniaeformis.

Scanning electron microscopy of the outer surface coat of the infective eggs of Hydatigera taeniaeformis examined at high magnifications revealed the presence of scale-like features. At low magnifications the surface of eggs appeared smooth. Eggs that were fractured showed a thick inner surface layer of ridges and striations. A second layer characterized by a smooth membrane surface presumably the basement membrane was observed beneath the innermost surface layer. When the eggs were treated with 0.02 M of EDTA the outer surface coat became distorted and the emerging hooks of the embryo could be seen. Small, spherical bosses were observed on the surface of some eggs. Other eggs possibly at an earlier stage of development contained pit-like depressions.

Animals↗
PubMed1979

The ultrastructural localization of alkaline phosphatase activity in the tegument of the cysticercus of Hydatigera taeniaeformis.

Alkaline phosphatase activity of the cysticercus of Hydatigera taeniaeformis was studied by means of electron microscopy. Beta-glycerophosphate was used as the substrate. The reaction product was localized within the microtriches of the distal cytoplasm, and in the cytoplasm and processes of perinuclear cells. Activity was also demonstrated within circular and longitudinal muscle bundles and in calcareous corpuscles.

Alkaline Phosphatase↗
PubMed1979

The ultrastructural localization of acetylcholinesterase activity in the scolex of the cysticercus of Hydatigera taeniaeformis.

Acetylcholinesterase activity in the scolex of the cysticercus of Hydatigera taeniaeformis was studied by means of electron microscopy. Acetylthiocholine iodide was used as the substrate and 10(-4) M of eserine used to inhibit acetylcholinesterase activity. The sites of activity were visualized within the microtriches of the distal cytoplasm, muscle bundles, cytoplasm, vesiculated bodies, flame cells and hooks of the rostellar region. Enzyme activity was also localized within the nuclear membrane, neuroplasm, and membranes of nerve cells.

Acetylcholinesterase↗
PubMed1975

A study of thyroid stimulating activity in human serum with the highly sensitive cytochemical bioassay.

The cytochemical bioassay is able to detect minute amounts of thyroteopin and thyroid stimulating immunoglobulins. Estimations of serum TSH by the cytochemical bioassay are in good agreement with values obtain by radioimmunoassay. In untreated hyperthyroid patients with Graves' disease the TSH levels are significantly lower than in control subjects. Thyroid stimulating immunoglobulins showed a delayed time corse of action in the bioassy and this provided a voncenient method of distinguishing between the two thyroid stimulators.

Animals↗