Biomedical subjects

B F Watkins

Publications and source records attributed to B F Watkins.

3 recordsLinked to original sources

PubMed1978

Effect of in vitro hemolysis on chemical values for serum.

Hemolysis in serum specimens is commonplace. This study examines the effect of hemolysis on results of selected chemical assays. Hemolysis was simulated by adding hemolysate to serum to give hemoglobin concentrations of 90 to 2800 mg/liter and a rating by technologists of 0 to 4 + hemolyzed. The effect of hemolysis on values for some serum constituents, particularly acid phosphatase and creatine kinase, was shown to be method dependent. Not unexpectedly, hemolysis most affects results for lactate dehydrogenase.

Acid Phosphatase↗

PubMedDate not supplied

Enzymes in amniotic fluid.

The determination of enzyme levels in cell-free amniotic fluid has proven useful in assessing fetal maturity and fetal well being, and is being utilized for the prenatal diagnosis of genetic disorders. The activities of amylase, alpha-galactosidase, phosphatidic acid phosphohydrolase, lysozyme and heat-stable alkaline phosphatase in amniotic fluid increase with gestational age and have an established relationship to fetal maturity. The ratio of amniotic fluid diamine oxidase activity to maternal serum activity (amniotic DAO/serum DAO) may be used as an indicator of the degree of rhesus isoimmunization after 28 weeks gestation. Creatine phosphokinase in amniotic fluid is elevated in cases of in utero fetal death and is of diagnostic significance. The prenatal diagnosis of Tay-Sachs disease, Sandhoff's disease, fucosidosis, GM1-gangliosidosis and I-cell disease have been made from the analysis of appropriate enzymes in cell-free amniotic fluid.

Alkaline Phosphatase↗

PubMedDate not supplied

An evaluation of a spectrophotometric scanning technique for measurement of plasma hemoglobin.

The measurement of plasma hemoglobin is utilized in the evaluation of various hemolytic disorders. The purpose of this report is to illustrate the advantages of a direct spectrophotometric scanning technique for determination of of plasma hemoglobin. This method does not require the use of carcinogenic reagents. The scanning method demonstrates linearity, precision, and sensitivity suitable for clinical use and is not affected by the presence of bilirubin.

Benzidines↗