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B Forghani

Publications and source records attributed to B Forghani.

60 records · Page 4Linked to original sources
PubMed1975

Type specificity of complement-requiring and immunoglobulin M neutralizing antibody in initial herpes simplex virus infections of humans.

Studies comparing the enhancing effect of guinea pig complement on homotypic and heterotypic neutralizing antibodies produced in initial herpes simplex virus (HSV) infections of humans indicated that antibodies to HSV type 1 and HSV type 2 were enhanced to about the same extent, and there was no signigicant difference in the degree to which complement enhanced homotypic and heterotypic HSV-neutralizing antibody. Homotypic and heterotypic immunoglobulin G neutralizing antibodies were enhanced by complement to as great, or greater, an extent as immunoglobulin M (IgM) HSV antibodies in the same sera. In patients with initial HSV type 1 infections, the IgM neutralizing antibody response was type specific. On the other hand, patients with initial HSV type 2 infections produced both homotypic and heterotypic IgM neutralizing antibody. An initial HSV type 2 infection in an individual previously infected with HSV type 1 elicited the production of IgM neutralizing antibody to both HSV type 1 and HSV type 2. However, patients with recurrent HSV type 1 infections failed to produce IgM antibody to either HSV type during reactivation of the virus.

Antibodies, Viral↗
PubMed1975

Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera.

An indirect solid phase radioimmunoassay (RIA) was developed for typing antibody to herpes simplex virus (HSV) types 1 and 2 (HSV-1 and HSV-2) in human sera. The test is based upon absorption of sera with uninfected, HSV-1-infected cells and testing for residual antibody. The high sensitivity of the RIA method for detecting HSV antibody permits examination of sera at high dilutions, and thus relatively small volumes of virus-infected cells are required for cross-absorption of antibodies. Results obtained in RIA typing of HSV antibodies showed good agreement with those obtained by microneutralization and inhibition of passive hemagglutination. The HSV antibody type(s) determined by RIA also showed good correlation with the virus type isolated from the individual, either from clinical specimens or sensory nerve ganglia. The technique was very sensitive for detection and typing of HSV antibdodies in cerebrospinal fluids. The RIA method was highly suitable for detecting two types of HSV antibody in the same serum specimen, and it was possible to show that a marked, type-specific antibody response to HSV-2 does occur in individuals with a primary HSV-2 infection who have experienced a prior infection with HSV-1.

Antibodies, Viral↗
PubMed1974

Solid phase radioimmunoassay for identification of Herpesvirus hominis types 1 and 2 from clinical materials.

A solid phase radioimmunoassay (RIA) was developed for typing Herpesvirus hominis (HVH) strains isolated from clinical materials, and it also proved to be applicable to the direct detection and typing of HVH antigen in human and animal brain tissue. The procedure utilized virus-infected human fetal diploid cells or brain tissue smears in the bottom of 1-dram glass vials, antigen was detected through the use of intermediate HVH antisera produced in rabbits or hamsters and cross-absorbed with the HVH heterotype, and (125)I-labeled anti-species (rabbit or hamster) globulins produced in goats were used for detection of immune complexes. The cross-absorbed HVH antisera could be used at high dilutions in the RIA test, and they reacted with marked type-specificity in the RIA system. Specificity of the test was also improved by determining and using optimal concentrations of intermediate sera and of (125)I-labeled anti-species globulins. Results of typing HVH isolates by the RIA procedure agreed in all instances with those obtained by direct fluorescent antibody staining with cross-absorbed conjugates. The RIA procedure was effective and more sensitive than direct fluorescent antibody for demonstrating and typing HVH antigen directly in smears of infected human brain tissue.

Animals↗