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Biomedical subjects

B Fransson

Publications and source records attributed to B Fransson.

At least 19 recordsLinked to original sources
PubMed2006

Primary uterine inertia in 27 bitches: aetiology and treatment.

OBJECTIVES: To evaluate two treatment methods in bitches with primary uterine inertia in relation to blood concentrations of oxytocin, calcium and glucose. METHODS: A prospective study where 27 bitches with primary inertia were randomly allocated to treatment groups I and II. Blood samples were collected before and after treatment. Group I was treated with a combination of intravenous calcium solutions and oxytocin and group II with oxytocin only. If unsuccessful, caesarean sections or additional medical treatment was pursued. RESULTS: Plasma oxytocin concentrations were 35+/-15 pmol/l in group I and 30+/-15 pmol/l in group II. Before treatment, the total serum calcium concentrations were 2.1+/-0.2 mmol/l in both the groups, and blood glucose values were 7.0+/-0.5 mmol/l (group I) and 7.3+/-1.4 mmol/l (group II). The number of bitches subjected to caesarean sections and the number of puppies born did not differ between the two groups. CLINICAL SIGNIFICANCE: This study indicates that low plasma oxytocin levels is a cause of primary inertia in bitches with normal serum calcium concentrations and aggravates the condition in bitches with low calcium levels. Hypoglycaemia was not observed. The outcome of labour did not differ between groups I and II.

Animals↗
PubMed1999

A physiotherapy test package for assessing back and neck dysfunction--discriminative ability for patients versus healthy control subjects.

BACKGROUND AND PURPOSE: A physiotherapy test package of 10 tests designed for assessing dysfunction due to back or neck pain was tested for its ability to discriminate between back- or neck-pain sufferers and healthy control subjects. Twenty-seven patients and 26 healthy control subjects participated in the study. METHOD: Fifteen patients suffering from back or neck pain and 15 matched healthy control subjects were assessed using the package to compare the results between the groups. The results from a further 12 back- or neck-pain sufferers and 11 unmatched healthy control subjects were analysed with respect to sensitivity and specificity, cluster formation and correlation with ratings of pain and perceived exertion. RESULTS: The differences in the test results for the patients and the matched healthy control subjects were highly significant in all variables but one. High sensitivity for cut-off points of clinical relevance was found in all variables but one, whereas specificity was moderate in most variables. CONCLUSIONS: Despite the small sample size, the significant differences between patients and healthy control subjects, with the sensitivity and specificity figures, suggest that the content validity of the test package is satisfactory.

Adult↗
PubMed1999

Determination of the optical purity of threonine and hydroxyproline by capillary gas chromatography on a chiral stationary phase.

Experimental conditions for the derivatization and resolution by GLC of all stereoisomers of threonine and 4-hydroxyproline are reported. Threonine was in two steps converted to N,O-bisisobutoxycarbonyl 2,2,2-trifluoroethyl ester derivatives, the second of which was performed under anhydrous conditions. As such the enantiomers could pairwise be separated by capillary gas chromatography on a Chirasil-Val column. Since L- and D-threonine eluted much earlier than the corresponding allo forms, quantitative determination of the allothreonine content in D- or L-threonine down to the one percent level could be simply accomplished but also enantiomeric impurities could be determined. Unlike for threonine, the corresponding 4-hydroxyproline isomers could not all be resolved as N,O-bisisobutoxycarbonyl 2,2,2-trifluoroethyl esters on this column. Although diastereomers could still be separated, the allo pair cochromatographed and the resolution for the L- and D-isomers was low. Complete separation of the 4-hydroxyproline isomers could be accomplished as N,O-bisprotected isobutyl amides, the formation of which required three derivatization steps. These were used for the determination of allohydroxyproline.

Chromatography, Gas↗
PubMed1997

Bacteriological findings, blood chemistry profile and plasma endotoxin levels in bitches with pyometra or other uterine diseases.

Uteri from 60 bitches with a clinical diagnosis of pyometra, or with an enlarged uterus as revealed radiographically or ultrasonographically, underwent histopathological examination, at which a diagnosis of pyometra was established in 48 of the 60 (80%) cases. Escherichia coli was isolated from 43 (90%) of the 48 uteri with pyometra. In 8 of the 60 cases, other pathological uterine conditions, such as endometrial hyperplasia, adenomyosis, mucometra or hydrometra, were diagnosed histopathologically. No bacterial growth was observed in the uteri of these 8 cases. Four of the 60 bitches (6%) showed no pathological changes in the uterus, and in 3 of these no bacteriological growth was seen in the uterus, while in one case a sparse growth of mixed culture was found. Blood samples from bitches with uterine infection caused by gram-negative bacteria showed marked hematological changes. These included higher total WBC counts and a more marked left shift in the differential WBC count than among the other bitches. Toxic degeneration of neutrophils was present among the bitches with gram-negative uterine infection and the serum ALP level was slightly higher than in the other groups of bitches. The plasma endotoxin concentration was determined in 53 bitches before surgery, in 28 bitches after surgery and in 11 control dogs. Only in 7 of the samples was endotoxin detected. The general condition of the bitches included in the present study was only mildly to moderately affected, and in no case indicated severe endotoxemia.

Animals↗
PubMed1994

Use of a dehydroalanine-containing peptide as an efficient inhibitor of tripeptidyl peptidase II.

Tripeptidyl peptidase II is an intracellular exopeptidase, which has been purified from rat liver and human erythrocytes. An efficient specific inhibitor was obtained through beta-elimination of phosphate from the phosphopeptide Arg-Ala-Ser(P)-Val-Ala. The dehydroalanine-containing peptide formed was a competitive inhibitor with a Ki of 0.02 +/- 0.01 microM. This study demonstrated that replacing a serine residue in a good inhibitor with a dehydroalanine residue reduced the Ki 45 times. It is proposed that dehydroalanine-containing peptides could be of interest in the development of inhibitors for other peptidases as well.

Alanine↗
PubMed1994

Synthesis and antiviral activity of three pyrazole analogues of distamycin A.

The synthesis of three new monopyrazole analogues of the antiviral compound distamycin A is reported. Suitably protected 4-amino-1-methylpyrrole-2-carboxylic acid and 3-amino-1-methylpyrazole-5-carboxylic acid derivatives were chosen as starting materials. The construction of the trimeric polyamide framework was accomplished by assembly of the monomeric precursors under condensing conditions by analogy with our previous methodology, although with significant improvements in some pivotal steps. After chromatographic purification and spectroscopic characterisation, the analogues were assayed for antiviral activity. Compounds 7a-c inhibited vaccinia virus at a concentration similar to or lower than distamycin A and the related antibiotic netropsin. Analogues 7b and 7c exhibited an antiviral effect comparable to those of distamycin A and netropsin against HSV-1 and HSV-2, whereas their antiviral activity against several other viruses including HIV-1 and HIV-2 was somewhat lower. The cellular toxicity of 7a-c toward different host cell types proved to be of similar magnitude or lower than those of distamycin A and netropsin.

Animals↗
PubMed1993

Wear resistance of some prosthodontic materials in vivo.

The purpose of this study was to compare a gravimetric method and an impression technique in the evaluation of occlusal substance loss. The wear of gold, porcelain, and microfilled resin was studied in vivo. The gravimetric method showed lower substance loss for porcelain than for gold, whereas the microfilled resin had the highest substance loss. To obtain a higher accuracy for the measurement of occlusal substance loss of restorative materials with an impression technique, the test area has to be restricted, the antagonizing occlusal contacts carefully recorded before the test period, and the number of cuts increased. The observed structure of wear facets (SEM) corroborated with previous findings of the wear mechanism of these materials; that is, gold has mainly abrasive wear in contact with porcelain, whereas porcelain has a fatigue type and microfilled resin a tribochemical type of wear.

Chemical Phenomena↗
PubMed1990

Separation of a complete set of basic, diastereomeric pentapeptides by reversed-phase ion-pair chromatography.

The resolving power of liquid chromatography systems containing 1-pentane sulfonate, previously used to analyze basic hydrophilic peptides related to substrates of cAMP-dependent protein kinase, has been studied with respect to diastereomeric peptides. A set of peptides comprising RRASV and its five diastereomers containing one D-amino acid were used as model compounds. Complete resolution of all the peptides could be accomplished both with ethanol and acetonitrile as organic modifiers. The separation of the various peptides within the set turned out to be only modestly changed under the conditions investigated. These new results clearly demonstrate the potential of the chromatography systems studied.

Amino Acid Sequence↗
PubMed1979

Oral recognition of forms and oral muscular coordination ability. A longitudinal study in young adults.

A study has been carried out to investigate the constancy of results in oral recognition of forms (RF-test) and in tests which demand a fine coordination of the oral muscular apparatus (MA-test). Twenty examinees with a mean age of 22.5 years were tested with both test forms in 1972 and retested 4 years later in 1976. The performances in the RF- as well as in the MA-test improved considerably. As to the learning (training) effect in both test forms, the test results acquired by training during the previous period have largely been preserved until the next test period 4 years later. Individuals with a given capacity to perform either the RF- or MA-test, showed about the same grade of ability 4 years later. The RF- as well as the MA-test seem to reflect a given ability, which, at least in this age-group, remained constant during a period of 4 years. These results may be explained by reference to the notion of schema in which some motor-sensory feed-back will be stored. The specificity of the perceptual and motor patterns used, may have produced schemata with good resistance against forgetting.

Adult↗
PubMed1976

Ion-pair chromatography of acidic drug metabolites and endogenic compounds.

Liquid-liquid chromatographic systems based on ion-pair partition with silica microparticles as the support for the stationary phase have been used for the separation of anionic compounds of biochemical and pharmacological interest. A high separating efficiency can be obtained with both aqueous and organic mobile phases and the retention is easily regulated by the nature and the concentration of the quaternary ammonium counter ion, present in the aqueous phase. The influence of the composition of the liquid phases on the selectivity and separating efficiency has been studied, as well as equilibration methods and the stability of the systems. Examples are given of separations of sulphonamides, barbiturates, glucuronic and sulphuric acid conjugates of steroidal compounds and phenols glycine conjugates of carboxylic acids (hippuric, nicotinuric and salicyluric acid) and anionic metabolites of biogenic amines (indoleacetic, benzoic, mandelic and phenylacetic acid derivatives).

Barbiturates↗
PubMed1975

Oral ability to recognize forms and oral muscular coordination ability in dentulous young and elderly adults.

A study has been carried out to investigate possible between a group of dentulous young adults (average 22-5 years of age) and a group of dentulous elderly individuals (65-9 years), when attempting to recognize forms orally and to carry out tasks which demand a fine-coordination of the oral muscular apparatus. The results showed, that the older group had reduced ability for both forms of tests used in this study (RF-test for estimating the oral ability to recognize forms, and MA-test to estimate oral muscular ability). The reduced capacity of the older individuals to learn the requirements of the tests as compared with the younger ones, was especially obvious. Furthermore, individual differences in this respect were more obvious for the older than for the younger examinees. The suggestion is made that in further studies the number of trial-rounds may be reduced from three to two, certainly for older individuals and for both the RF- and the MA-test. Because of the obvious correlation between the results of the RF- and the MA-test among older individuals, it is concluded that both forms of tests may measure common or closely related factors, which are reflected by these tests.

Adult↗