2-Iminobiotin-containing reagent and affinity columns.
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Avidin↗
Biomedical subjects
B Fudem-Goldin
Publications and source records attributed to B Fudem-Goldin.
Synthesis and rapid purification of UDP-[6-3H]galactose.
UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.
Chromatography, Ion Exchange↗