Biomedical subjects

B G Casslén

Publications and source records attributed to B G Casslén.

4 recordsLinked to original sources

PubMed1991

Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology.

The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2 alpha (PGF2 alpha) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2 alpha by epithelial cells. We conclude that the secretion of PGF2 alpha from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.

Animals↗

PubMed1990

Progesterone regulation of prolactin release from human endometrial stromal cells in culture: potential bioassay for progestational activity.

Prolactin was produced by endometrial stromal cells, but not by epithelial cells, in primary culture. Production was stimulated by progesterone in a time and dose (1-1000 nmol/l) dependent way. Stimulation was started one day after plating. The release of prolactin started one day earlier in cultures from luteal phase than proliferative phase endometria. The responsiveness to progesterone declined with time in culture, sooner in luteal than in proliferative phase cultures, but could, at least partly, be maintained by including estradiol (10 nmol/l) in the medium. Lost response to progesterone could be restored by stimulation with estradiol for two days. The results indicate that prolactin synthesis in the endometrium is regulated directly by progesterone, and indirectly by estradiol. We suggest that prolactin production in primary stromal cell cultures could serve as a bioassay for progestational activity of steroid hormones in the human endometrium.

Adult↗

PubMed1989

Platelet-activating factor in human luteal phase endometrium.

Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is one of the most potent mediators of vascular permeability. PAF levels change in the rabbit endometrium just prior to implantation, which suggests that PAF may be a key substance transducing preimplantation embryonic signals. To study whether PAF was present in the human endometrium, and if so, to determine the cellular origin and hormonal regulation of endometrial PAF, specimens were obtained from 14 women (aged 23-42 yr) undergoing elective hysterectomy during the luteal phase of the cycle (plasma progesterone levels greater than 2 ng/ml). No specimens were taken from women with malignant uterine pathology. Stromal cells and epithelial glandular cells were separated by collagenase and DNAse digestion, and then cultured to confluence in vitro in medium 199. Radioimmunoassays of prostaglandin F (PGF) and prolactin in the culture media were used to confirm cell type and viability. PGF release into the culture medium from stromal cells was low (control 1.52 +/- 0.20 ng/ml), and unchanged by hormone treatment. In contrast, release of PGF from unstimulated glandular cells was 6.05 +/- 0.52 ng/ml, and was significantly increased (p less than 0.05) by estradiol or progesterone plus estradiol, to 12.17 +/- 1.67, and 8.60 +/- 0.81, respectively. Progesterone alone was without effect. Prolactin was secreted by stromal cell cultures, increasing steadily from 24 to 120 h. The levels in the medium were increased by progesterone. PAF activity was assessed by rabbit platelet aggregation and serotonin-release bioassays after lipid extraction and separation by thin-layer chromatography.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1987

Free amino acids in human uterine fluid. Possible role of high taurine concentration.

Human uterine fluid (UF) was found to contain several amino acids at high concentrations. Taurine was predominant; its concentration in UF was up to 400-fold that of plasma during the luteal phase. High UF concentrations of amino acids probably result from active endometrial secretion, which is selective with respect to type of amino acid and is regulated by ovarian steroids. Amino acids, particularly taurine, have an apparent function in osmotic regulation since UF has a lower salt content than serum but the same osmolarity. The high concentration of taurine may also act to sustain the motility of spermatozoa in UF, by protecting them from the adverse influence of high K+ concentration. Knowledge of the amino acid composition of UF in the luteal phase may prove useful in such clinical contexts as in vitro fertilization of ova and embryo replacement.

Amino Acids↗