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Biomedical subjects

B G Kovrov

Publications and source records attributed to B G Kovrov.

18 recordsLinked to original sources

Interaction of physical-chemical and biological regeneration processes in ecological Life Support Systems.

Catalytic combustion of inedible biomass of plants in ecological Life Support Systems (LSS) gives rise to gaseous oxides (CO2, NO2, SO2, etc.). Some of them are toxic for plants suppressing their photosynthesis and productivity. Experiments with "Bios-3" experimental LSS demonstrate that a decrease of photosynthetic productivity in a system with straw incineration can jeopardize its steady operation. Analysis of the situation by a mathematical model taking into account absorption parameters of the system in terms of toxic elements makes it possible to formulate requirements for the structure and operation of LSS to provide for its stability. Avenues for further investigation of the problem of toxic stability of LSS are proposed.

Air Conditioning↗

Long-term experiments on man's stay in biological life-support system.

We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as wall as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions.

Air Conditioning↗

[Express method of studying microorganism interaction on solid media].

To study interaction of microogranisms on solid media, a method of agar plates with the use of replicators was elaborated and compared with that of perpendicular streaks and agar blocks. Apart from being a rapid one, the new method provides quantitative determination of the interaction of level of the microbes. The results of the method are reliable and easily reproduced. Out of the 3 procedures considered the method of agar plates is most sensitive and precise.

Agar↗

[Dependence of the rate of ferrous oxide oxidation by a Thiobacillus ferrooxidans culture on its concentration].

The effect of the concentration of ferrous iron on the rate of its oxidation was studied with Thiobacillus ferrooxidans. The specific rate of oxidation of ferrous iron was found to increase with its concentration and to decrease with a rise of the total iron content. Cultivation of bacteria with electrochemical reduction of Fe3+ produced high cell concentrations at a low content of total iron (4--6 g/litre), e. g. upto 4.5 g/litre (dry biomass weight).

Culture Media↗

[Dynamics of the accumulation and retention of volatile organic substances released by bacteria].

The object of this work was to study the production of volatile biologically active compounds by microorganisms and their accumulation in the medium in the course of several days. The inhibiting action of the accumulated volatile metabolites was found to depend on the time within which the culture was incubated and on the sensitivity of the test organism. The activity of the accumulated metabolites was shown to decrease with the time of storage. Several volatile compounds were found among the metabolites.

Anti-Bacterial Agents↗

[Composition of a nutrient medium for the continuous cultivation of Thiobacillus ferrooxidans].

Thiobacillus ferrooxidans was found to require the following biogenous elements for constructing its biomass: phosphorus, 14 +/- 2; magnesium, 2 +/- 0.5; potassium, 5 +/- 1; nitrogen, 100 +/- 10 (milligrams per gram of dry biomass). It was shown that the concentrations of biogenous elements in the growth medium, which did not limit the biosynthesis, did not exceed 5 divided by 10 mg per litre for all of the studied elements. The medium 9K that is commonly used for growing Thiobacillus ferrooxidans has been found to differ significantly from the calculated one with the balanced ratio of biogenous elements.

Calcium↗