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Biomedical subjects

B Graham

Publications and source records attributed to B Graham.

47 records · Page 3Linked to original sources
PubMed1976

Immunocompetent cells in man. IV. The inhibition of pokeweed mitogen-induced blastogenesis of normal human peripheral leukocytes by trypsin: a B-cell mitogen of mouse, but not of human, lymphocytes.

Normal human peripheral blood lymphocytes were tested for their blastogenic response in vitro to trypsin. Although it has been shown by other investigators that mouse B lymphocytes are stimulated trypsin to undergo blastogenesis, we have shown that human lymphocytes are not stimulated by trypsin. Paradoxically, trypsin inhibits the response of human lymphocytes to pokeweed mitogen, a B-cell stimulat, without significantly affecting the response of these cells to phytohemagglutinin, a T-cell stimulant. These results serve to underline the functional dissimilarity of mouse spleen cells and human circulating lymphocytes, at least with respect to their blastogenic responses to mitogenic agents.

Animals↗
PubMed1976

Purification of herpesvirus nucleocapsids by fluorocarbon extraction.

Purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with Freon 113. Fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of DNA. CsCl banded DNA represented 30--40% of the theoretical yield of the total nucleocapsid population.

Capsid↗
PubMed1975

Chronic toxicity of a synthetic tri-aryl phosphate oil to fish.

Rainbow trout (Salmo gairdneri) were exposed to IMOL S-140, a synthetic high-temperature lubricating oil composed of tri-aryl phosphates and, although no sign of acute toxicity was evident, the fish slowly developed symptoms of chronic posioning. Thus, floating food pellets were refused, the activities of glutamic oxalacetic transaminase and lactate dehydrogenase in serum were greatly elevated, and internal fatty tissues became discoloured. Neither serum nor brain cholinesterase activities were inhibited.

Adipose Tissue↗
PubMedDate not supplied

Supracondylar osteotomy of the humerus for correction of cubitus varus.

Reports of corrective procedures for cubitus varus (gunstock deformity) have emphasized the frequency of loss of position and significant complication. Modifications that have been made to the standard supracondylar osteotomy protocol include correction of the angular deformity only, without correction of rotation; an incomplete wedge osteotomy leaving the medial cortex intact by hinging the osteotomy shut on the intact cortex; and immobilization of the elbow in full extension with the forearm supinated. In a review of 16 patients following this protocol, 11 patients had excellent results, three had good results, and two had unsatisfactory results.

Bone Nails↗